Non-nutritive sugar feeding alters the genetic profile of diuretic hormones and their receptors in Drosophila suzukii (Diptera: Drosophilidae)

Annals of the Entomological Society of America, Год журнала: 2025, Номер unknown

Опубликована: Май 8, 2025

Abstract Non-nutritive sugars such as erythritol and sucralose are active ingredients in artificial sweeteners. In the past decade, research has demonstrated insecticidal properties of non-nutritive across several arthropod species. It is now a prospective chemical control option for spotted-wing drosophila, Drosophila suzukii, one most economically important pests small fruits. D. suzukii die quicker, excrete waste large amounts, become desiccated when fed mixture sucralose. The toxicity to fly not clearly elucidated but likely due nutritional physiological failures associated with an excess fluid excretion water imbalance, caused hyperosmotic body. diuretic processes insects primarily regulated by 3 neuropeptides: CAPA hormone, hormone 31 (DH31), 44 (DH44). To explore genetic effects on we investigated regulation key genes—capa, dh31, dh44—and their 7 corresponding receptors under different dietary conditions: + sucrose, Molecular analyses revealed that expression levels capa, dh44, were significantly altered sugar-fed flies compared group only sucrose. Through trials testing synergistic peptide injection sugar consumption, DH44 consistently increased mortality all diet groups. These findings offer molecular insights development novel pest management strategies targeting suzukii.

Язык: Английский

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Diuresis and α-glucosidase inhibition by erythritol in Aedes aegypti (Diptera: Culicidae) and viability for efficacy against mosquitoes

Parasites & Vectors, Год журнала: 2024, Номер 17(1)

Опубликована: Фев. 20, 2024

Sugar alcohols, such as erythritol, are low-impact candidates for attractive toxic sugar baits (ATSB) to kill mosquitoes. To determine whether erythritol has a viable future in ATSB formulations, suite of assays was conducted diagnose toxicity mechanisms and starvation effects on mortality Aedes aegypti (L.) model system. We measured general carbohydrate load, glucosidase levels, free glucose intoxicated adult mosquitoes observe digestion impaired. assayed the combinations with larvae adults. measure when were not resource-deprived, additional manipulated prior status. Up 50,000 ppm water had no effect within 72 h, but an ammonia spike indicated diuresis early 4 h (F8,44 = 22.50, P < 0.0001) after sucrose/erythritol added. Adult consumption diuretic regardless pairing, while sucrose together generated above 80% (F2,273 33.30, alongside triple normal excretion (F5,78 26.80, 0.0004). Glucose fructose paired individually less mortality, still double fecal excretion. When ingesting erythritol-laced meals, detected compared meals (χ2 12.54, df 1, Data showed that is linear competitive inhibitor α-glucosidase, marking it novel class insecticide current research climate. However, efficacy null persistent across various levels. Despite significant diuresis, combined from acute enough vector control programs considering against

Язык: Английский

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Effect of Erythritol and Sucralose Formulation on the Survivorship of the Mosquito Aedes aegypti

Florida Entomologist, Год журнала: 2023, Номер 106(2)

Опубликована: Июнь 22, 2023

Se demostró que el eritritol, un poliol de 4 carbonos no nutritivo, tiene propiedades insecticidas para múltiples plagas artrópodos. Las formulaciones eritritol combinadas con sacarosa, como fagoestimulante, mejoraron consumo y la eficacia insecticida sobre las plagas. Sin embargo, agregar sacarosa aporta recursos nutricionales carbohidratos en mismas plagas, puede conducir al desarrollo microbios campo crear residuo pegajoso plantas rociadas solución. En este estudio, probamos comparamos o sucralosa, e identificamos actividades adultos del mosquito Aedes aegypti. La formulación sucralosa es una solución menos pegajosa redujo significativamente sobrevivencia los mosquitos adultos, tener mérito método alternativo control justifica evaluación adicional.

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Insecticidal Properties of Erythritol on Four Tropical Tephritid Fruit Flies, Zeugodacus cucurbitae, Ceratitis capitata, Bactrocera dorsalis, and B. latifrons (Diptera: Tephritidae)

Insects, Год журнала: 2023, Номер 14(5), С. 472 - 472

Опубликована: Май 16, 2023

Tephritid fruit flies are among the most destructive agricultural pests of fruits and vegetables worldwide can impose trade barriers against movement fresh tropical commodities. Primary pre-harvest control methods for these rely on spraying conventional chemical insecticides or bait sprays. However, resistance to has been reported in flies. Erythritol is a non-nutritive sugar alternative human consumption, which tested confirmed its insecticidal properties various insect pest species. In this study, using laboratory bioassays, we evaluated effect erythritol alone formulations containing sucrose and/or protein four fly species established Hawaii (e.g., melon fly, Mediterranean oriental Malaysian fly). addition, effects other hexose pentose alcohols, such as sorbitol, mannitol, xylitol, were tested. Among different standalone combinatory treatments tested, 1M formulation 2M + 0.5M appeared be detrimental survival all flies, suggesting potential non-toxic management tool tephritid

Язык: Английский

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Effects of nonnutritive sugar inclusion in laboratory diets and attracticidal spheres on survivorship and mobility of 2 Dipteran species, Rhagoletis pomonella (Diptera: Tephritidae) and Drosophila suzukii (Diptera: Drosophilidae)

Journal of Economic Entomology, Год журнала: 2024, Номер 117(2), С. 595 - 600

Опубликована: Янв. 24, 2024

Abstract Native apple maggot fly, Rhagoletis pomonella, and invasive spotted-wing drosophila, Drosophila suzukii, are key pests of small fruit, respectively, in the United States. Both species typically managed with standard insecticide applications. However, interest alternative strategies that result reductions has led to evaluations nonnutritive sugars as toxicants for development attracticidal spheres both species. Here, we evaluated survivorship R. pomonella D. suzukii when provided diets substituted saccharin, sucralose, aspartame, erythritol, dextrose, or mannitol sucrose component compared them water-only controls up 15 days. Presence erythritol significantly decreased suzukii. mobility trials following a 2 h exposure aqueous solutions each sugar treatment resulted no strong impact on either Survivorship after 30 min alone, combination varying concentrations (serving phagostimulant) at 24 were Only was affected erythritol:sucrose 20:0% 15:5% h. Based all results, appeared most promising, integrated into toxicant but even highest concentration, remained unaffected species, thus making this impractical ineffective substitute spheres.

Язык: Английский

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Effects of nonnutritional sugars on lipid and carbohydrate content, physiological uptake, and excretion in Drosophila suzukii

Archives of Insect Biochemistry and Physiology, Год журнала: 2021, Номер 109(2)

Опубликована: Дек. 5, 2021

The nonnutritive sugar, erythritol, has the potential to be a human-safe management tool for small fruits and cherry pest, Drosophila suzukii, or spotted-wing drosophila. Feeding on erythritol decreases fly survival oviposition by starving creating an osmotic imbalance in body. Recently, we demonstrated that combined with another sucralose, was fed upon more than alone hastens D. suzukii mortality. This suggests sucralose is suitable phagostimulant alternative sucrose. Although promising, nutritional physiological impacts of are unknown. In this study, investigated whether metabolized excreted when various sucrose, formulations. We found cannot converted into any substitutes storage carbohydrates suzukii. Instead, molecules were largely accumulated hemolymph slowly from body, significant To excrete unused sugars, flies will use their own body fluids restore homeostasis, resulting losing substantial amount weight becoming desiccated process. summary, ingesting leads starvation hyperosmotic pressure causing decrease fitness. With confirmation being non-metabolizable phagostimulative erythritol+sucralose formulation promising insecticide growers use.

Язык: Английский

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Nonnutritive Sugars for Spotted-Wing Drosophila (Diptera: Drosophilidae) Control Have Minimal Nontarget Effects on Honey Bee Larvae, a Pupal Parasitoid, and Yellow Jackets

Environmental Entomology, Год журнала: 2022, Номер 52(1), С. 47 - 55

Опубликована: Ноя. 16, 2022

Abstract Drosophila suzukii Matsumura, spotted-wing drosophila, is a major pest of small fruits and cherries often managed with conventional insecticides. Our previous work found that erythritol, nonnutritive polyol, has insecticidal properties to D. suzukii. Two formulations erythritol (1.5M), 0.5M sucrose or 0.1M sucralose, are most effective at killing In this study, we investigated the nontarget effects these on honey bee Apis mellifera Linnaeus larvae, pupal parasitoid suzukii, Pachycrepoideus vindemiae Rondani, western yellow jacket, Vespula pensylvanica Saussure. We directly exposed larvae by adding high dose (2 µl) larval cells no significant mortality from either formulation compared water control. may encounter in field settings when host plants sprayed. The erythritol+sucralose was more detrimental than erythritol+sucrose P. vindemiae, however, effect greatly reduced within 21-d period floral source present. Since jackets nuisance were attracted recent trials, tested adult V. survival continuous consumption laboratory. detectable detriment formulation, Overall, both caused minimal parasitoids, jackets.

Язык: Английский

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Erythritol sprays reduce Drosophila suzukii infestation without impacting honey bee visitation nor fruit quality

Pest Management Science, Год журнала: 2023, Номер 79(12), С. 4990 - 5002

Опубликована: Авг. 4, 2023

Abstract BACKGROUND Spotted‐wing drosophila, Drosophila suzukii , is an economic pest of small fruits and cherries. Insecticides primarily control this while alternative controls are in development. Laboratory studies show that erythritol insecticidal to D. other pests approved for human consumption. Moreover, combined with sucrose or non‐caloric sucralose can stimulate feeding quicken mortality. Before growers use erythritol, the impact on crop protection, non‐target insects, fruit quality need evaluation. RESULTS In three blueberry cherry field cage trials, oviposition sprayed erythritol:sucrose erythritol:sucralose formulations was lowered by 59%–81% compared unsprayed controls. Fly infestation (larval adult counts from fruit) 90% lower a greenhouse trial, 49% open trial 2 m : 0.5 sucrose. Infestation also 57% 1.5 erythritol:0.5 Other trials very low pressure frequent rains revealed no differences Field consistently honey bees did not preferentially visit plants either formulation, although yellow jackets visited more frequently. Erythritol led leaf spotting, but there reduction treated blueberries cherries terms mold development, firmness, diameter, epidermal penetration force, Brix° (total soluble solids) at harvest. CONCLUSION Eleven conducted over four years reduce without attracting foraging nor negatively impacting quality. © 2023 Society Chemical Industry. This article has been contributed U.S. Government employees their work public domain USA.

Язык: Английский

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Evaluating potential phytotoxicity of erythritol solution, a novel control method for Drosophila suzukii, in blueberry, cherry, and wild Himalayan blackberry

Crop Protection, Год журнала: 2024, Номер unknown, С. 106961 - 106961

Опубликована: Сен. 1, 2024

Язык: Английский

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