Water,
Год журнала:
2022,
Номер
14(8), С. 1224 - 1224
Опубликована: Апрель 11, 2022
Onsite
molecular
diagnostics
can
revolutionize
fecal
pollution
source
tracking.
We
aimed
to
validate
a
method
for
onsite
qPCR
assays
with
miniature
speaker-sized
Q
instrument
and
other
portable
equipment
items.
showed
that
marker
genes
total
bacteria
(16S)
E.
coli
(rodA)
in
100
mL
of
river
water
measured
this
agreed
within
±0.3
log10
units
results
obtained
when
using
conventional
laboratory
then
deployed
the
mobile
(‘lab
van’)
quantified
HF183
human
host
associated
Bacteroides
3
h
sampling.
also
used
investigate
urban
effluents
from
two
storm
drains
retention
pond
collected
comprehensive
microbial
physicochemical
quality
data.
found
significantly
higher
gene
levels
older
drain
compared
(6.03
±
0.04
vs.
4.23
0.03
copies
per
mL),
principal
component
analysis
revealed
effluent
beneficially
altered
characteristics,
making
them
more
like
those
receiving
river.
In
conclusion,
be
performed
items
quickly
test
water.
Water,
Год журнала:
2021,
Номер
13(24), С. 3551 - 3551
Опубликована: Дек. 12, 2021
With
increasing
concerns
about
public
health
and
the
development
of
molecular
techniques,
new
detection
tools
combination
existing
approaches
have
increased
abilities
pathogenic
bacteria
monitoring
by
exploring
biomarkers,
sensitivity
accuracy
detection,
quantification,
analyzing
various
genes
such
as
functional
antimicrobial
resistance
(ARG).
Molecular
methods
are
gradually
emerging
most
popular
approach
for
pathogens,
in
addition
to
conventional
culture-based
plate
enumeration
methods.
The
analysis
pathogens
wastewater
back-estimation
infections
community,
also
known
wastewater-based
epidemiology
(WBE),
is
an
methodology
has
a
great
potential
supplement
current
surveillance
systems
infectious
diseases
early
warning
outbreaks.
However,
complex
matrix,
largely
challenges
analytical
performance
This
review
synthesized
literature
typical
wastewater,
types
bacterial
analysis,
their
recent
advances
analysis.
advantages
limitation
these
were
evaluated,
prospects
WBE
discussed
provide
insight
future
development.
Current Opinion in Environmental Science & Health,
Год журнала:
2022,
Номер
27, С. 100348 - 100348
Опубликована: Март 4, 2022
Amid
the
2019
coronavirus
disease
pandemic
(COVID-19),
scientific
community
has
a
responsibility
to
provide
accessible
public
health
resources
within
their
communities.
Wastewater
based
epidemiology
(WBE)
been
used
monitor
spread
of
pandemic.
The
goal
this
review
was
evaluate
need
for
an
environmental
justice
approach
COVID-19
WBE
starting
with
state
California
in
United
States.
Methods
included
peer-reviewed
literature,
government-provided
data,
and
news
stories.
As
June
2021,
there
were
twelve
universities,
nine
dashboards,
48
384
wastewater
treatment
plants
monitoring
SARS-CoV-2
California.
majority
conducted
urban
areas
Coastal
Southern
(34/48),
lack
more
rural
Central
(10/48)
Northern
(4/48).
Similar
access
clinical
testing
vaccinations,
is
disparity
which
can
often
early
warning
system
outbreaks.
This
research
demonstrates
equity
considerations
when
determining
locations
monitoring.
Abstract
Real‐time
polymerase
chain
reaction
(RT‐PCR)
remains
the
most
prevalent
molecular
detection
technology
for
sewage
analysis
but
is
plagued
with
numerous
disadvantages,
such
as
time
consumption,
high
manpower
requirements,
and
susceptibility
to
false
negatives.
In
this
study,
an
automated
robot‐driven
photoelectrochemical
(PEC)
biosensing
platform
constructed,
that
utilizes
CRISPR/Cas12a
system
achieve
fast,
ultrasensitive,
specificity
of
biological
loads
in
sewage.
The
Shennong‐1
robot
integrates
several
functional
modules,
involving
sampling
pretreatment
streamline
monitoring.
A
screen‐printed
electrode
employed
a
vertical
graphene‐based
working
enhanced
surface‐deposited
Au
nanoparticles
(NPs).
CdTe/ZnS
quantum
dots
(QDs)
are
further
fabricated
through
double‐stranded
DNA
(dsDNA)
anchored
on
NPs.
Using
cDNA
template
Omicron
BA.5
spike
gene
model,
PEC
biosensor
demonstrates
excellent
analytical
performance,
lower
limit
2.93
×
10
2
z
m
outstanding
selectivity
at
level
single‐base
mutation
recognition.
Furthermore,
rapid,
accurate
feasibility
conclusion,
allows
early
tracking
infectious
disease
outbreaks,
providing
timely
data
support
public
health
institutions
take
appropriate
prevention
control
measures.
Abstract
Microfluidic
Paper‐based
Analytical
Devices
(µPADs)
have
emerged
as
a
new
class
of
microfluidic
systems,
offering
numerous
advantages
over
traditional
chips.
These
include
simplicity,
cost‐effectiveness,
stability,
storability,
disposability,
and
portability.
As
result,
various
designs
for
different
types
assays
are
developed
investigated.
In
recent
years,
µPADs
combined
with
conventional
detection
methods
to
enable
rapid
on‐site
detection,
providing
results
comparable
expensive
sophisticated
large‐scale
testing
that
require
more
time
skilled
personnel.
The
application
µPAD
techniques
is
extensive
in
environmental
quality
control/analysis,
clinical
diagnosis,
food
safety
testing,
paving
the
way
real‐time
diagnosis
promising
future
development.
This
review
focuses
on
research
advancements
design,
fabrication,
material
selection,
µPADs.
It
provides
comprehensive
understanding
their
principles
operation,
applications,
development
prospects.
Applied and Environmental Microbiology,
Год журнала:
2022,
Номер
88(5)
Опубликована: Янв. 5, 2022
Wastewater-based
surveillance
is
a
powerful
tool
that
provides
an
unbiased
measure
of
COVID-19
prevalence
in
community.
This
work
describes
sensitive
wastewater
rapid
test
for
SARS-CoV-2
based
on
widely
distributed
technology,
the
GeneXpert.
