The Science of The Total Environment,
Год журнала:
2024,
Номер
915, С. 170174 - 170174
Опубликована: Янв. 19, 2024
Bees
carry
out
vital
ecosystem
services
by
pollinating
both
wild
and
economically
important
crop
plants.
However,
while
performing
this
function,
bee
pollinators
may
encounter
potentially
harmful
xenobiotics
in
the
environment
such
as
pesticides
(fungicides,
herbicides
insecticides).
Understanding
key
factors
that
influence
toxicological
outcomes
of
exposure
to
these
chemicals,
isolation
or
combination,
is
essential
safeguard
their
health
they
provide.
In
regard,
recent
work
using
toxicogenomic
phylogenetic
approaches
has
begun
identify,
at
molecular
level,
determinants
pesticide
sensitivity
pollinators.
These
include
detoxification
systems
convert
less
toxic
forms
residues
insecticide
target-sites
underlie
species-specific
selectivity.
Here
we
review
emerging
body
research
summarise
state
knowledge
We
identify
gaps
our
for
future
examine
how
an
understanding
genetic
basis
can
be
leveraged
to,
a)
predict
avoid
negative
bee-pesticide
interactions
facilitate
development
pest-selective
bee-safe
insecticides,
b)
inform
traditional
effect
assessment
risk
address
issues
ecotoxicological
concern.
Environment International,
Год журнала:
2021,
Номер
147, С. 106372 - 106372
Опубликована: Янв. 7, 2021
Honey
bee
(Apis
mellifera)
first-tier
pesticide
risk
assessment
is
largely
based
on
standardized
laboratory
toxicity
bioassays
after
both
acute
and
chronic
exposure.
Recent
research
honey
cytochrome
P450
monooxygenases
(P450s)
uncovered
CYP9Q3
as
the
molecular
determinant
mediating
neonicotinoid
insecticide
selectivity
explaining
why
certain
neonicotinoids
such
thiacloprid
show
>
1000-fold
lower
than
others
(e.g.
imidacloprid).
Here
this
knowledge
leveraged
for
mechanistic
at
level
using
a
fluorescence-based
high-throughput
in
vitro
assay,
predicting
interaction
of
diverse
pesticidal
chemotypes,
including
azole
fungicides,
with
recombinantly
expressed
CYP9Q
enzymes,
known
to
metabolize
thiacloprid,
acetamiprid
tau-fluvalinate.
Some
fungicides
were
shown
be
synergistic
combination
insecticides,
pyrethroids,
whereas
prothioconazole
not.
We
demonstrate
that
biochemical
CYP9Q2/CYP9Q3
inhibition
data
azoles
revealed
striking
correlation
their
potential
organismal
level,
even
allow
explain
combined
effects
observed
tank
mixtures
under
field
conditions.
Our
novel
toxicogenomics-based
approach
designed
complement
existing
methods
unprecedented
screening
capacity,
by
utilizing
enzymes
confer
selectivity,
order
biochemically
address
issues
ecotoxicological
concern.
Proceedings of the National Academy of Sciences,
Год журнала:
2023,
Номер
120(37)
Опубликована: Сен. 5, 2023
Here,
we
provide
mechanistic
support
for
the
involvement
of
CYP9A
subfamily
cytochrome
P450
monooxygenases
in
detoxification
host
plant
defense
compounds
and
chemical
insecticides
Spodoptera
exigua
frugiperda
.
Our
comparative
genomics
shows
that
a
large
cluster
genes
occurs
two
species
but
with
significant
differences
its
contents,
including
several
species-specific
duplicates
substantial
sequence
divergence,
both
between
orthologs
duplicates.
Bioassays
CRISPR-Cas9
knockouts
clusters
show
that,
collectively,
CYP9As
can
detoxify
furanocoumarin
(imperatorin
xanthotoxin)
representing
three
different
chemotypes
(pyrethroids,
avermectins,
oxadiazines).
However,
vitro
metabolic
assays
heterologously
expressed
products
individual
particular
activities
against
these
compounds.
We
also
find
tight
genetic
linkage
high
levels
pyrethroid
resistance
field
strains
species.
propose
their
divergent
amplifications
have
not
only
contributed
to
development
broad
ranges
over
long
evolutionary
timeframes
supplied
them
diverse
options
evolving
very
recent
past.
The Science of The Total Environment,
Год журнала:
2024,
Номер
915, С. 170174 - 170174
Опубликована: Янв. 19, 2024
Bees
carry
out
vital
ecosystem
services
by
pollinating
both
wild
and
economically
important
crop
plants.
However,
while
performing
this
function,
bee
pollinators
may
encounter
potentially
harmful
xenobiotics
in
the
environment
such
as
pesticides
(fungicides,
herbicides
insecticides).
Understanding
key
factors
that
influence
toxicological
outcomes
of
exposure
to
these
chemicals,
isolation
or
combination,
is
essential
safeguard
their
health
they
provide.
In
regard,
recent
work
using
toxicogenomic
phylogenetic
approaches
has
begun
identify,
at
molecular
level,
determinants
pesticide
sensitivity
pollinators.
These
include
detoxification
systems
convert
less
toxic
forms
residues
insecticide
target-sites
underlie
species-specific
selectivity.
Here
we
review
emerging
body
research
summarise
state
knowledge
We
identify
gaps
our
for
future
examine
how
an
understanding
genetic
basis
can
be
leveraged
to,
a)
predict
avoid
negative
bee-pesticide
interactions
facilitate
development
pest-selective
bee-safe
insecticides,
b)
inform
traditional
effect
assessment
risk
address
issues
ecotoxicological
concern.
