bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Янв. 7, 2023
Locomotor
movements
cause
visual
images
to
be
displaced
across
the
eye,
a
retinal
slip
that
is
counteracted
by
stabilizing
reflexes
in
many
animals.
In
insects,
optomotor
turning
causes
animal
turn
direction
of
rotating
stimuli,
thereby
reducing
and
trajectories
through
world.
This
behavior
has
formed
basis
for
extensive
dissections
motion
vision.
Here,
we
report
under
certain
stimulus
conditions,
two
Drosophila
species,
including
widely
studied
D.
melanogaster,
can
suppress
even
reverse
response
over
several
seconds.
Such
"anti-directional
turning"
most
strongly
evoked
long-lasting,
high-contrast,
slow-moving
stimuli
are
distinct
from
those
promote
syn-directional
turning.
Anti-directional
turning,
like
response,
requires
local
detecting
neurons
T4
T5.
A
subset
lobula
plate
tangential
cells,
CH
show
involvement
these
responses.
Imaging
variety
direction-selective
cells
shows
no
evidence
dynamics
match
behavior,
suggesting
observed
inversion
emerges
downstream
plate.
Further,
anti-directional
declines
with
age
exposure
light.
These
results
behaviors
contain
rich,
stimulus-dependent
inconsistent
simple
reflexive
stabilization
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 31, 2023
Summary
Visual
systems
are
homogeneous
structures,
where
repeating
columnar
units
stereotypically
arranged
to
retinotopically
cover
the
visual
field.
Each
of
these
columns
contain
many
same
neuron
types
that
distinguished
by
anatomic,
genetic
and
–
generally
functional
properties.
However,
there
exceptions
this
rule.
In
800
Drosophila
eye,
is
an
anatomically
genetically
identifiable
cell
type
with
variable
properties,
Tm9.
Since
anatomical
connectivity
shapes
neuronal
we
identified
presynaptic
inputs
several
hundred
Tm9s
across
both
optic
lobes
using
FAFB
connectome
dataset
FlyWire
analysis.
Our
work
shows
Tm9
has
three
major,
stereotypic,
weaker,
sparsely
distributed
inputs.
This
differs
from
neurons
uniform
Tm1
Tm2,
which
have
only
one
more
stereotypic
than
Within
heterogeneous
circuit
architecture,
specific
motifs,
such
as
a
set
wide-field
neurons,
can
be
source
physiology.
Genetic
synapse
labeling
combined
expansion
microscopy
showed
wiring
exists
individuals.
Together,
our
data
argue
system
uses
heterogeneous,
properties
achieve
robust
processing.
iScience,
Год журнала:
2020,
Номер
23(6), С. 101170 - 101170
Опубликована: Май 17, 2020
Visual
systems
of
many
animals,
including
the
fruit
fly
Drosophila,
represent
surrounding
space
as
2D
maps,
formed
by
populations
neurons.
Advanced
genetic
tools
make
visual
system
especially
well
accessible.
However,
in
typical
vivo
preparations
for
two-photon
calcium
imaging,
relatively
few
neurons
can
be
recorded
at
same
time.
Here,
we
present
an
extension
to
a
conventional
microscope,
based
on
remote
focusing,
which
enables
real-time
rotation
imaging
plane,
and
thus
flexible
alignment
cellular
structures,
without
resolution
or
speed
trade-off.
We
simultaneously
record
from
over
100
neighboring
cells
spanning
retinotopic
map.
characterize
its
representation
moving
natural
images,
find
is
comparable
noise
predictions.
Our
method
increases
throughput
10-fold
allows
us
visualize
significant
fraction
fly's
field.
Furthermore,
our
applied
general
more
investigation
neural
circuits.
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2021,
Номер
unknown
Опубликована: Ноя. 4, 2021
Abstract
Color
and
motion
are
used
by
many
species
to
identify
salient
moving
objects.
They
processed
largely
independently,
but
color
contributes
processing
in
humans,
for
example,
enabling
colored
objects
be
detected
when
their
luminance
matches
the
background.
Here,
we
demonstrate
an
unexpected,
additional
contribution
of
vision
Drosophila
.
We
show
that
behavioral
ON-motion
responses
more
sensitive
UV
than
OFF-motion,
cellular
pathways
connecting
UV-sensitive
R7
photoreceptors
ON
OFF-motion-sensitive
T4
T5
cells,
using
neurogenetics
calcium
imaging.
Remarkably,
synergy
enhances
detection
approaching
discs,
not
green
discs
with
same
chromatic
contrast,
how
this
generalizes
visual
systems
OFF
pathways.
Our
results
provide
a
computational
circuit
basis
favor
saliently
bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Янв. 7, 2023
Locomotor
movements
cause
visual
images
to
be
displaced
across
the
eye,
a
retinal
slip
that
is
counteracted
by
stabilizing
reflexes
in
many
animals.
In
insects,
optomotor
turning
causes
animal
turn
direction
of
rotating
stimuli,
thereby
reducing
and
trajectories
through
world.
This
behavior
has
formed
basis
for
extensive
dissections
motion
vision.
Here,
we
report
under
certain
stimulus
conditions,
two
Drosophila
species,
including
widely
studied
D.
melanogaster,
can
suppress
even
reverse
response
over
several
seconds.
Such
"anti-directional
turning"
most
strongly
evoked
long-lasting,
high-contrast,
slow-moving
stimuli
are
distinct
from
those
promote
syn-directional
turning.
Anti-directional
turning,
like
response,
requires
local
detecting
neurons
T4
T5.
A
subset
lobula
plate
tangential
cells,
CH
show
involvement
these
responses.
Imaging
variety
direction-selective
cells
shows
no
evidence
dynamics
match
behavior,
suggesting
observed
inversion
emerges
downstream
plate.
Further,
anti-directional
declines
with
age
exposure
light.
These
results
behaviors
contain
rich,
stimulus-dependent
inconsistent
simple
reflexive
stabilization
