Horizontal-cell like Dm9 neurons in Drosophila modulate photoreceptor output to supply multiple functions in early visual processing DOI Creative Commons
Christopher Schnaitmann,

Manuel Pagni,

Patrik B. Meyer

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: May 15, 2024

Dm9 neurons in Drosophila have been proposed as functional homologs of horizontal cells the outer retina vertebrates. Here we combine genetic dissection neuronal circuit function, two-photon calcium imaging and inner photoreceptors, immunohistochemical analysis to reveal novel insights into role early visual processing. Our experiments show that receive input from all four types photoreceptor R7p, R7y, R8p, R8y. Histamine released R7/R8 directly inhibits via histamine receptor Ort, outweighs simultaneous histamine-independent excitation by UV-sensitive R7. turn provides inhibitory feedback R7/R8, which is sufficient for color-opponent processing R7 but not R8. Color opponent R8 requires additional synaptic inhibition same ommatidium axo-axonal synapses second HisCl1. Notably, optogenetic prohibits color decreases intracellular terminals. The latter likely results reduced release excitatory glutamate shifts overall sensitivity toward higher light intensities. In summary, our underscore a key suggest regulating adaptation photoreceptors. These findings on are indeed reminiscent versatile functions vertebrate retina.

Language: Английский

Seeing Natural Images through the Eye of a Fly with Remote Focusing Two-Photon Microscopy DOI Creative Commons
Anna Schuetzenberger, Alexander Borst

iScience, Journal Year: 2020, Volume and Issue: 23(6), P. 101170 - 101170

Published: May 17, 2020

Visual systems of many animals, including the fruit fly Drosophila, represent surrounding space as 2D maps, formed by populations neurons. Advanced genetic tools make visual system especially well accessible. However, in typical vivo preparations for two-photon calcium imaging, relatively few neurons can be recorded at same time. Here, we present an extension to a conventional microscope, based on remote focusing, which enables real-time rotation imaging plane, and thus flexible alignment cellular structures, without resolution or speed trade-off. We simultaneously record from over 100 neighboring cells spanning retinotopic map. characterize its representation moving natural images, find is comparable noise predictions. Our method increases throughput 10-fold allows us visualize significant fraction fly's field. Furthermore, our applied general more investigation neural circuits.

Language: Английский

Citations

6
Synergy of color and motion vision for detecting approaching objects inDrosophila DOI Creative Commons
Kit D. Longden, Edward M. Rogers, Aljoscha Nern

et al.

bioRxiv (Cold Spring Harbor Laboratory), Journal Year: 2021, Volume and Issue: unknown

Published: Nov. 4, 2021

Abstract Color and motion are used by many species to identify salient moving objects. They processed largely independently, but color contributes processing in humans, for example, enabling colored objects be detected when their luminance matches the background. Here, we demonstrate an unexpected, additional contribution of vision Drosophila . We show that behavioral ON-motion responses more sensitive UV than OFF-motion, cellular pathways connecting UV-sensitive R7 photoreceptors ON OFF-motion-sensitive T4 T5 cells, using neurogenetics calcium imaging. Remarkably, synergy enhances detection approaching discs, not green discs with same chromatic contrast, how this generalizes visual systems OFF pathways. Our results provide a computational circuit basis favor saliently

Language: Английский

Citations

6
Connectomics: From form to function in butterfly colour vision DOI Creative Commons
Anna Stöckl

Current Biology, Journal Year: 2022, Volume and Issue: 32(10), P. R459 - R461

Published: May 1, 2022

Language: Английский

Citations

3
Super-Performance: Sampling, Planning, and Ecological Information DOI
Bradly Alicea

Studies in computational intelligence, Journal Year: 2024, Volume and Issue: unknown, P. 33 - 40

Published: Jan. 1, 2024

Language: Английский

Citations

0
Horizontal-cell like Dm9 neurons in Drosophila modulate photoreceptor output to supply multiple functions in early visual processing DOI Creative Commons
Christopher Schnaitmann,

Manuel Pagni,

Patrik B. Meyer

et al.

Frontiers in Molecular Neuroscience, Journal Year: 2024, Volume and Issue: 17

Published: May 15, 2024

Dm9 neurons in Drosophila have been proposed as functional homologs of horizontal cells the outer retina vertebrates. Here we combine genetic dissection neuronal circuit function, two-photon calcium imaging and inner photoreceptors, immunohistochemical analysis to reveal novel insights into role early visual processing. Our experiments show that receive input from all four types photoreceptor R7p, R7y, R8p, R8y. Histamine released R7/R8 directly inhibits via histamine receptor Ort, outweighs simultaneous histamine-independent excitation by UV-sensitive R7. turn provides inhibitory feedback R7/R8, which is sufficient for color-opponent processing R7 but not R8. Color opponent R8 requires additional synaptic inhibition same ommatidium axo-axonal synapses second HisCl1. Notably, optogenetic prohibits color decreases intracellular terminals. The latter likely results reduced release excitatory glutamate shifts overall sensitivity toward higher light intensities. In summary, our underscore a key suggest regulating adaptation photoreceptors. These findings on are indeed reminiscent versatile functions vertebrate retina.

Language: Английский

Citations

0