Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila DOI Open Access
Katharina Eichler, Stefanie Hampel,

Adrián Alejandro-García

и другие.

Опубликована: Май 31, 2023

Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that Drosophila perform a location-prioritized grooming sequence when at different locations on head are simultaneously stimulated by dust (Hampel et al., 2017; Seeds 2014). Here, we identify nearly all individually aimed of specific locations, while collectively eliciting whole sequence. Different tracing methods were used reconstruct projections these distinct arborizations in brain. This provides first synaptic resolution somatotopic map head, defines parallel-projecting pathways grooming.

Язык: Английский

A Drosophila computational brain model reveals sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

и другие.

Nature, Год журнала: 2024, Номер 634(8032), С. 210 - 219

Опубликована: Окт. 2, 2024

Язык: Английский

Процитировано

24
Descending neurons coordinate anterior grooming behavior in Drosophila DOI Creative Commons
Li Guo, Neil Zhang, J. Simpson

и другие.

Current Biology, Год журнала: 2022, Номер 32(4), С. 823 - 833.e4

Опубликована: Фев. 1, 2022

The brain coordinates the movements that constitute behavior, but how descending neurons convey myriad of commands required to activate motor limbs in right order and combinations produce those is not well understood. For anterior grooming behavior fly, we show its component head sweeps leg rubs can be initiated separately, or as a set, by different neurons. Head are mutually exclusive front legs normally alternate, circuits ventral nerve cord resolve competing commands. Finally, left must work together remove debris. coordination for achieved unilateral activation single neuron, while similar manipulation neuron decouples single-sided sweeps. Taken together, these results demonstrate distinct orchestrate complex alternation between make up grooming.

Язык: Английский

Процитировано

43
Cross-talk between immunity and behavior: insights from entomopathogenic fungi and their insect hosts DOI Creative Commons
Wei Zhang, Xuanyu Chen, Ioannis Eleftherianos

и другие.

FEMS Microbiology Reviews, Год журнала: 2024, Номер 48(1)

Опубликована: Янв. 1, 2024

Abstract Insects are one of the most successful animals in nature, and entomopathogenic fungi play a significant role natural epizootic control insect populations many ecosystems. The interaction between insects has continuously coevolved over hundreds millions years. Many components innate immune responses against fungal infection conserved across phyla. Additionally, behavioral responses, which include avoidance, grooming, and/or modulation body temperature, have been recognized as important mechanisms for opposing pathogens. In an effort to investigate possible cross-talk mediating these fundamental biological processes, recent studies integrated explored responses. Current information indicates that during discrete stages infection, several altered simultaneously, suggesting connections two systems. This review synthesizes advances our understanding physiological molecular aspects influencing antifungal reactions, including chemical perception olfactory pathways.

Язык: Английский

Процитировано

16
Sensing of a spore surface protein by a Drosophila chemosensory protein induces behavioral defense against fungal parasitic infections DOI Creative Commons
Junmei Shang, Guirong Tang, Jun Yang

и другие.

Current Biology, Год журнала: 2022, Номер 33(2), С. 276 - 286.e5

Опубликована: Ноя. 23, 2022

In addition to innate immunity in a physiological context, insects have evolved behavioral defenses against parasite attacks. Here, we report that Drosophila can sense the CFEM (common fungal extracellular membrane) protein Mcdc9, which acts as negative virulence factor of entomopathogenic fungus Metarhizium robertsii. The individual deletions 18 genes followed by fly infection identified three null mutants could kill flies more quickly than wild-type strain, among Mcdc9 coat spores and interact with chemosensory CheA75a. deletion or knockdown CheA75a had similar effect, greater number were left on respective controls after topical infection. Thus, accelerated death treated ΔMcdc9, CheA75aRNAi succumbed control topically challenged strain. gene is highly transcribed legs wings, positive electrophysiological responses evidenced tarsal sensilla stimulation protein. results imply this be sensed contact elicitor inducing hygienic behavior parasitic infection, reveals previously unsuspected mechanism fungus-insect interactions.

Язык: Английский

Процитировано

31
Synaptic connectome of a neurosecretory network in the Drosophila brain DOI Creative Commons
Theresa H. McKim, Jayati Gera,

Ariana Julia B. Gayban

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Авг. 29, 2024

Abstract Hormones mediate inter-organ signaling which is crucial in orchestrating diverse behaviors and physiological processes including sleep activity, feeding, growth, metabolism reproduction. The pars intercerebralis lateralis insects represent major hubs contain neurosecretory cells (NSC) that produce various hormones. To obtain insight into how hormonal regulated, we have characterized the synaptic connectome of NSC adult Drosophila brain. Identification neurons providing inputs to multiple subtypes implicates diuretic hormone 44-expressing as a coordinator physiology behavior. Surprisingly, despite most having dendrites subesophageal zone (primary taste processing center), gustatory are largely indirect. We also deciphered pathways via olfactory relayed NSC. Further, our analyses revealed substantial from descending NSC, suggesting regulate both endocrine motor output synchronize changes with appropriate behaviors. In contrast inputs, sparse mostly mediated by corazonin Therefore, additionally determine putative paracrine interconnectivity between peripheral tissues analyzing single-cell transcriptomic datasets. Our comprehensive characterization network provides platform understand complex networks they orchestrate animal physiology.

Язык: Английский

Процитировано

6
Distinct subpopulations of mechanosensory chordotonal organ neurons elicit grooming of the fruit fly antennae DOI Creative Commons
Stefanie Hampel, Katharina Eichler, Daichi Yamada

и другие.

eLife, Год журнала: 2020, Номер 9

Опубликована: Окт. 26, 2020

Diverse mechanosensory neurons detect different mechanical forces that can impact animal behavior. Yet our understanding of the anatomical and physiological diversity these behaviors they influence is limited. We previously discovered grooming Drosophila melanogaster antennae elicited by an antennal chordotonal organ, Johnston’s organ (JO) (Hampel et al., 2015). Here, we describe anatomically physiologically distinct JO neuron subpopulations each elicit grooming. show project to different, discrete zones in brain differ their responses stimulation antennae. Although activation subpopulation elicits grooming, also additional wing flapping or backward locomotion. Our results provide a comprehensive description JO, reveal both common behavioral responses.

Язык: Английский

Процитировано

33
A leaky integrate-and-fire computational model based on the connectome of the entire adultDrosophilabrain reveals insights into sensorimotor processing DOI Creative Commons
Philip K. Shiu, Gabriella R Sterne, Nico Spiller

и другие.

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2023, Номер unknown

Опубликована: Май 2, 2023

Abstract The forthcoming assembly of the adult Drosophila melanogaster central brain connectome, containing over 125,000 neurons and 50 million synaptic connections, provides a template for examining sensory processing throughout brain. Here, we create leaky integrate-and-fire computational model entire brain, based on neural connectivity neurotransmitter identity, to study circuit properties feeding grooming behaviors. We show that activation sugar-sensing or water-sensing gustatory in accurately predicts respond tastes are required initiation. Computational region those elicit motor neuron firing, testable hypothesis validate by optogenetic behavioral studies. Moreover, different classes makes accurate predictions how multiple taste modalities interact, providing circuit-level insight into aversive appetitive processing. Our sugar water pathways form partially shared initiation pathway, which our calcium imaging experiments confirm. Additionally, applied this mechanosensory circuits found small set comprising antennal do not overlap with circuits, describes response upon subtypes. results demonstrate modeling purely from predicted identity generates experimentally hypotheses can describe complete sensorimotor transformations.

Язык: Английский

Процитировано

12
A pair of commissural command neurons induces Drosophila wing grooming DOI
Neil Zhang, J. Simpson

iScience, Год журнала: 2022, Номер 25(2), С. 103792 - 103792

Опубликована: Фев. 1, 2022

Язык: Английский

Процитировано

18
Somatotopic organization among parallel sensory pathways that promote a grooming sequence in Drosophila DOI Creative Commons
Katharina Eichler, Stefanie Hampel,

Adrián Alejandro-García

и другие.

eLife, Год журнала: 2023, Номер 12

Опубликована: Май 31, 2023

Mechanosensory neurons located across the body surface respond to tactile stimuli and elicit diverse behavioral responses, from relatively simple stimulus location-aimed movements complex movement sequences. How mechanosensory their postsynaptic circuits influence such behaviors remains unclear. We previously discovered that Drosophila perform a location-prioritized grooming sequence when at different locations on head are simultaneously stimulated by dust (Hampel et al., 2017; Seeds 2014). Here, we identify nearly all individually aimed of specific locations, while collectively eliciting whole sequence. Different tracing methods were used reconstruct projections these distinct arborizations in brain. This provides first synaptic resolution somatotopic map head, defines parallel-projecting pathways grooming.

Язык: Английский

Процитировано

9
DANCE: An open-source analysis pipeline and low-cost hardware to quantify aggression and courtship in Drosophila DOI Open Access

R Sai Prathap Yadav,

Pranab Dey,

Faizah Ansari

и другие.

Опубликована: Март 25, 2025

Quantifying animal behaviors is pivotal for identifying the underlying neuronal and genetic mechanisms. Computational approaches have enabled automated analysis of complex such as aggression courtship in Drosophila . However, existing rely on rigid, rule-based algorithms expensive hardware, limiting sensitivity to behavioral variations accessibility. Here, we describe DANCE ( Aggression Courtship Evaluator), a low-cost, open-source platform combining machine learning-based classifiers inexpensive hardware quantify courtship. consists six novel trained using supervised learning algorithm. outperform by capturing dynamic variations. constructed repurposed medicine blister packs acrylic sheets, with recordings performed smartphones, making it affordable accessible. Benchmarking demonstrated that performs comparably sophisticated, high-cost setups. We validated diverse contexts, including social isolation versus enrichment modulate courtship, RNAi-mediated down-regulation neuropeptide Dsk, optogenetic silencing dopaminergic neurons which promoted aggression. provides cost-effective portable solution studying resource-limited settings or closer natural habitats. Its accessibility robust performance democratizes neuroscience, enabling rapid screening genes circuits behaviors.

Язык: Английский

Процитировано

0