A brainstem integrator for self-location memory and positional homeostasis in zebrafish

Cell, Год журнала: 2022, Номер 185(26), С. 5011 - 5027.e20

Опубликована: Дек. 1, 2022

To track and control self-location, animals integrate their movements through space. Representations of self-location are observed in the mammalian hippocampal formation, but it is unknown if positional representations exist more ancient brain regions, how they arise from integrated self-motion, by what pathways locomotion. Here, a head-fixed, fictive-swimming, virtual-reality preparation, we exposed larval zebrafish to variety involuntary displacements. They tracked these displacements and, many seconds later, moved toward earlier location corrective swimming ("positional homeostasis"). Whole-brain functional imaging revealed network medulla that stores memory induces an error signal inferior olive drive future swimming. Optogenetically manipulating medullary integrator cells evoked displacement-memory behavior. Ablating them, or downstream olivary neurons, abolished displacement corrections. These results reveal multiregional hindbrain circuit vertebrates integrates self-motion locomotor

Язык: Английский

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Asymmetric mechanotransduction by hair cells of the zebrafish lateral line

Current Biology, Год журнала: 2023, Номер 33(7), С. 1295 - 1307.e3

Опубликована: Март 10, 2023

Язык: Английский

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Sensing in the dark: Constructive evolution of the lateral line system in blind populations of Astyanax mexicanus

Ecology and Evolution, Год журнала: 2024, Номер 14(4)

Опубликована: Апрель 1, 2024

Abstract Cave‐adapted animals evolve a suite of regressive and constructive traits that allow survival in the dark. Most studies aiming at understanding cave animal evolution have focused on genetics environmental underpinnings traits, with special emphasis vision loss. Possibly as result loss, other non‐visual sensory systems expanded compensated species. For instance, many cave‐dwelling fish species, including blind cavefish Mexican tetra, Astyanax mexicanus , major mechanosensory system called lateral line, for loss through morphological expansions. While substantial work has shed light adaptation this system, there are still open questions regarding its developmental origin, synaptic plasticity, overall adaptive value. This review provides snapshot current state knowledge line adaption A. an anatomy, behavior. Multiple avenues future research how these can be leveraged tools both evolutionary biology medicine, discussed.

Язык: Английский

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Optogenetic interrogation of the lateral-line sensory system reveals mechanisms of pattern separation in the zebrafish brain

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Фев. 8, 2025

Abstract The ability of animals to interact with their environment hinges on the brain’s capacity distinguish between patterns sensory information and accurately attribute them specific organs. mechanisms by which neuronal circuits discriminate encode source signals remain elusive. To address this, we utilized as a model posterior lateral line system larval zebrafish, is used detect water currents. This comprises series mechanosensory organs called neuromasts, are innervated neurons from ganglion. By combining single-neuromast optogenetic stimulation whole-brain calcium imaging, developed novel approach investigate how inputs neuromasts processed. Upon stimulating individual observed that in brain zebrafish show diverse selectivity properties despite lack topographic organization second-order circuits. We further demonstrated complex combinations neuromast represented sparse ensembles within medial octavolateralis nucleus (MON) found input can be integrated nonlinearly. Our offers an innovative method for spatiotemporally interrogating presents valuable studying encoding.

Язык: Английский

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Quantitative neuronal morphometry by supervised and unsupervised learning

STAR Protocols, Год журнала: 2021, Номер 2(4), С. 100867 - 100867

Опубликована: Сен. 30, 2021

We present a protocol to characterize the morphological properties of individual neurons reconstructed from microscopic imaging. first describe simple procedure extract relevant features digital tracings neural arbors. Then, we provide detailed steps on classification, clustering, and statistical analysis traced cells based features. illustrate pipeline design using specific examples zebrafish anatomy. Our approach can be readily applied generalized characterization axonal, dendritic, or glial geometry. For complete context scientific motivation for studies datasets used here, refer Valera et al. (2021).

Язык: Английский

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A zebrafish gephyrinb mutant distinguishes synaptic and enzymatic functions of Gephyrin

Neural Development, Год журнала: 2024, Номер 19(1)

Опубликована: Июль 27, 2024

Abstract Gephyrin is thought to play a critical role in clustering glycine receptors at synapses within the central nervous system (CNS). The main vivo evidence for this comes from ( Gphn )-null mice, where are depleted synaptic regions. However, these mice die birth, possibly due impaired molybdenum cofactor (MoCo) synthesis, an essential assumes throughout animal. This complicates interpretation of phenotypes -null and raises question whether enzymatic functions can be investigated separately. Here, we generated gephyrinb zebrafish mutant, vo84 , that almost entirely lacks staining spinal cord. mutants exhibit normal gross morphology both larval adult stages. In contrast motor activity MoCo-dependent enzyme activity. Instead, display rheotaxis increased mortality late development. To investigate what may mediate defects mutants, examined cell density neurons myelin cord found no obvious changes. Surprisingly, still present their abundance reduced, potentially contributing observed defects. These findings challenge notion absolutely required cluster reveals new regulating receptor rheotaxis. They also establish powerful model studying mechanisms underlying synaptic, rather than enzymatic, Gephyrin.

Язык: Английский

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Restoring Shank3 in the rostral brainstem of shank3ab−/− zebrafish autism models rescues sensory deficits

Communications Biology, Год журнала: 2021, Номер 4(1)

Опубликована: Дек. 17, 2021

Abstract People with Phelan-McDermid Syndrome, caused by mutations in the SHANK3 gene, commonly exhibit reduced responses to sensory stimuli; yet changes brain-wide activity that link these symptoms shank3 gene remain unknown. Here we quantify movement response sudden darkness larvae of two zebrafish mutant models and show both dampened this stimulus. Using mapping, find −/− light-sensing brain regions normal levels while sensorimotor integration motor are less active. Specifically restoring Shank3 function a nucleus rostral brainstem enables model respond like wild-type. In sum, responsiveness is associated processing can be rescued brainstem. These studies highlight importance for integrating inputs generate behavioral adaptations changing stimuli.

Язык: Английский

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A comprehensive characterizations of zebrafish rheotactic behaviors and its application to otoprotective drug screening

Expert Systems with Applications, Год журнала: 2023, Номер 237, С. 121496 - 121496

Опубликована: Сен. 7, 2023

Язык: Английский

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Multisensory strategies for postural compensation after lateral line loss

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2024, Номер unknown

Опубликована: Янв. 24, 2024

To control elevation underwater, aquatic vertebrates integrate multisensory information (e.g., vestibular, visual, proprioceptive) to guide posture and swim kinematics. Here we characterized how larval zebrafish changed locomotive strategies after imposed instability (decreased buoyancy) in the presence absence of visual cues. We discovered that larvae sank more acute loss lateral line (flow-sensing) hair cells. In response, engaged different compensatory strategies, depending on whether they were light or dark. dark, swam frequently, engaging their trunk steer nose up climb effectively. However, light, climbed often, both pectoral fins elevate. conclude sense use vestibular as available trajectory. Our work is a step towards understanding neural computations responsible for allow orientation navigation depth.

Язык: Английский

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Physiological responses of mechanosensory systems in the head of larval zebrafish (Danio rerio)

Frontiers in Robotics and AI, Год журнала: 2023, Номер 10

Опубликована: Июль 31, 2023

The lateral line system of zebrafish consists the anterior line, with neuromasts distributed on head, and posterior trunk. sensory afferent neurons are contained in ganglia, respectively. So far, vast majority physiological developmental studies have focused line. However, that focus especially its physiology, very rare. involves different neuromast patterning processes, specific distribution synapses, a unique role behavior. Here, we report our observations regarding development analyze responses to mechanical water jet stimuli. Sensing fish head may be crucial avoid obstacles, catch prey, orient current, absence visual cues. Alongside trigeminal system, fine nerve endings innervating skin, could contribute perceiving mechanosensory stimulation. Therefore, compare responsiveness auditory neurons. We show tuned velocity ramp stimulation, while either only respond step stimuli or fast Auditory did not These results prove essential designing underwater robots artificial lines, respect spectra systems larval to, underline importance functionality head.

Язык: Английский

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