Sensory adaptation at ribbon synapses in the zebrafish lateral line

The Journal of Physiology, Год журнала: 2021, Номер 599(15), С. 3677 - 3696

Опубликована: Май 28, 2021

Key points The present study aimed to determine the sensory adaptation characteristics of hair cell ribbon synapses in vivo . Hair cells zebrafish lateral line transmit hydrodynamic stimuli posterior ganglion afferent neurons. Excitatory bundle deflections by water‐jet cause glutamate release at with a rapid (phasic) and sustained component, which are likely linked exocytosis distinct vesicle pools. glutamate‐induced increase neuron firing rate adapts over time, is mirrored depression neurotransmitter release, without preventing phase‐locking. Adaptation also occurs during inhibitory displacements, highlighting shift sensitivity range prolonged stimulation. Postsynaptic mechanisms exert some degree regulation on adaptation. We conclude that depletion primary determinant adaptation, allowing maintain stimuli. Abstract used systems adjust continuously their match changes environmental In auditory vestibular systems, properties glutamate‐containing vesicles play crucial role thus shaping neural response How regulate how they modulate responses still largely unknown. Here, we have two‐photon imaging electrophysiology investigate synaptic transfer context live zebrafish. Prolonged repeated stimulation stereociliary bundles caused action potential elicited By monitoring using time‐lapse imaging, identified two kinetically components: was exhausted within 50–100 ms slower lasting entire After stimulations, recovery fast component followed biphasic time course. Depression responsible for recorded However, postsynaptic Ca 2+ had course compared indicating contribute exhibited form stimulations. optimised machinery encode new incoming

Язык: Английский

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A brainstem integrator for self-localization and positional homeostasis

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2021, Номер unknown

Опубликована: Ноя. 27, 2021

Abstract To accurately track self-location, animals need to integrate their movements through space. In amniotes, representations of self-location have been found in regions such as the hippocampus. It is unknown whether more ancient brain contain and by which pathways they may drive locomotion. Fish displaced water currents must prevent uncontrolled drift potentially dangerous areas. We that larval zebrafish can later swim back earlier location. Whole-brain functional imaging revealed circuit enabling this process positional homeostasis. Position-encoding brainstem neurons optic flow, then bias future swimming correct for past displacements modulating inferior olive cerebellar activity. Manipulation position-encoding or olivary abolished homeostasis evoked behavior if had experienced shifts. These results reveal a multiregional hindbrain vertebrates flow integration, memory its neural pathway behavior.

Язык: Английский

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Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish

Опубликована: Апрель 8, 2024

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. the lateral line sensory system of zebrafish, axons reorganize terminal arborization patterns to establish precise microcircuits around mechanosensory hair cells. However, quantitative understanding changes in arbor morphology and regulators behind microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator these processes. Utilizing semi-automated three-dimensional neurite tracing methodology computational techniques, have identified quantitatively analyzed distinct topological features shape network wild-type Sema7A loss-of-function mutants. contrast those animals, mutants display aberrant arborizations with disorganized topology diminished contacts Moreover, ectopic expression secreted form by non-hair cells induces chemotropic guidance axons. Our findings propose likely functions both juxtracrine cue pattern circuitry during organ development.

Язык: Английский

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Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish

Опубликована: Май 17, 2024

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. the lateral line sensory system of zebrafish, axons reorganize terminal arborization patterns to establish precise microcircuits around mechanosensory hair cells. However, quantitative understanding changes in arbor morphology and regulators behind microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator these processes. Utilizing semi-automated three-dimensional neurite tracing methodology computational techniques, have identified quantitatively analyzed distinct topological features shape network wild-type Sema7A loss-of-function mutants. contrast those animals, mutants display aberrant arborizations with disorganized topology diminished contacts Moreover, ectopic expression secreted form by non-hair cells induces chemotropic guidance axons. Our findings propose likely functions both juxtracrine cue pattern circuitry during organ development.

Язык: Английский

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In preprints: theme and variations on hair-cell regeneration in zebrafish

Development, Год журнала: 2024, Номер 151(14)

Опубликована: Июль 15, 2024

Imagine reaching old age and still being able to engage effortlessly in conversations (Cheslock De Jesus, 2023). This possibility hinges on preserving the mechanosensory hair cells we are born with, which mammals exceptionally long-lived but, with very few exceptions, cannot be replaced once lost (Kirkegaard Jørgensen, 2000; Savas, In humans, loss of these results chronic hearing deficits, making everyday activities such as conversing noisy settings or talking phone overwhelming challenges (Reynard Thai-Van, 2024). For many, this impairment not only isolates them from social interactions but also aggravates mental health issues anxiety depression (Luppa et al., Most insights into maintaining function come studying non-mammalian vertebrates, can regenerate naturally throughout their lives (Choi 2024; Denans 2019; Pinto-Teixeira 2013; Benkafadar Emerging research is revealing that multiple mechanisms regulate hair-cell regeneration, even within same species, offering hope for future breakthroughs treating humans.Among most studied hair-cell-bearing organs neuromasts lateral line, enable fishes amphibians detect low-frequency mechanical fluctuations surrounding water (Valera 2021; Tidswell These aquatic vertebrates use line orient relative flow direction, escape predators locate prey, develop avoidance reactions. The take a more prominent role when vision limited (Montgomery 2000). Fishes have ears, constituent control balance high-frequency signals (Liu Bagnall, Importantly, ears must remain functional life animal, despite persistent environmental insult cells. zebrafish has always offered researchers two key advantages (Barrallo-Gimeno Llorens, 2022; Plazas Elgoyhen, Holmgren Sheets, Pickett Raible, 2019). First, unparalleled accessibility high-resolution microscopy, chiefly thanks its superficial location availability many lines expressing various genetically encoded fluorescent makers label different cell types (Pinto-Teixeira 2015; Hewitt Second, quickly easily eliminated using pharmacological, genetic physical approaches. ensuing recovery occurs days, enabling high temporal-resolution recording entire regenerative process 2013). recent years, advances single-cell transcriptomics added above (Lush Baek Kozak 2020). Single-cell RNA sequencing (scRNA-seq) hairpin chain reaction-based situ hybridization (HCR-FISH) helped molecularly characterize cellular sub-populations (Shi Moreover, easy production mutant animals gives access molecular components drive regeneration (Parvez 2024).The initial discovery resident progenitors took place almost decades ago (López-Schier Hudspeth, 2006). Every study followed agreed general mechanism follows series steps start birth new pairs mitotic division unipotent (UHCP; Thomas Mackenzie 2012). Although it been established how supporting re-enter mitosis after death, direct UHCP behavior remained obscure (Kozak preprint, Bell colleagues evidence scRNA-seq experiments showing gene foxg1a expressed during (Bell 2024 preprint). They allele find reduced number neuromast formation. Using nls-Eos transgenic photoconvertible protein disentangle effect fox1ga mutants neuromast, they conclude Foxg1a controls proliferation population isl1a-expressing central progenitor cells.However, nagging issue field found ear zebrafish, readily (Jimenez 2022). There currently no known marker specific UHCP, unambiguous identification achieved live videomicroscopy Therefore, one explanation finding much difficult image live. Another described simply do exist ear. another Beaulieu show robust through markedly differs neuromasts, resembling closely birds (Beaulieu preprint; Bhave 1995). Here, ablation, arise transdifferentiation pool Transdifferentiation identity conversion without an immediate intervening (Wang traditionally term reserved interconversion between belonging lineages, agree authors preprint sensu lato would entail any postmitotic another. transient wave precursor otherwise depleted by continuous maintain stable preprint) used data HCR-FISH distinguish location: cabp1b-positive recently differentiated peripheral, whereas scn5lab-expressing located centrally ear's crista. A clever combination capsaicin-mediated ablation system allowed measure possible alternative interpretation inner produces pairs, does, progenitor. Then, identically birds, immediately takes mature identity, sibling remains hidden dormant immature state until further death induces emergence (Stone Rubel, Regardless, interesting what mammalian and, therefore, may clinically relevant humans.Together, preprints highlight need combine studies understand regeneration. will benefit taking advantage scRNA-seq-derived data, together newly-developed phiC31 Integrase Genomic Loci Engineered Transgenesis (pIGLET) generate diverse reproducible expression patterns compare populations behave (Lalonde Brown 2023).Beaulieu al. (2024 now accepted as: Beaulieu, M. O., Thomas, E. D. W. (2024). temporally uncoupled expansion Development 151, dev202944. doi:10.1242/dev.202944.

Язык: Английский

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Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish

eLife, Год журнала: 2024, Номер 12

Опубликована: Авг. 12, 2024

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. the lateral line sensory system of zebrafish, axons reorganize terminal arborization patterns to establish precise microcircuits around mechanosensory hair cells. However, quantitative understanding changes in arbor morphology and regulators behind microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator these processes. Utilizing semi-automated three-dimensional neurite tracing methodology computational techniques, have identified quantitatively analyzed distinct topological features shape network wild-type Sema7A loss-of-function mutants. contrast those animals, mutants display aberrant arborizations with disorganized topology diminished contacts Moreover, ectopic expression secreted form by non-hair cells induces chemotropic guidance axons. Our findings propose likely functions both juxtracrine cue pattern circuitry during organ development.

Язык: Английский

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Representation of bulk water flow in the goldfish (Carassius auratus) midbrain

Journal of Comparative Physiology A, Год журнала: 2024, Номер unknown

Опубликована: Сен. 17, 2024

Язык: Английский

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Lateral line: From water waves to brain waves

Current Biology, Год журнала: 2021, Номер 31(7), С. R344 - R347

Опубликована: Апрель 1, 2021

Язык: Английский

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Reviewer #4 (Public Review): Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish

Опубликована: Сен. 12, 2023

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. the lateral line sensory system of zebrafish, axons reorganize terminal arborization patterns to establish precise microcircuits around mechanosensory hair cells. However, quantitative understanding changes in arbor morphology and regulators behind microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator these processes. Utilizing semi-automated three-dimensional neurite tracing methodology computational techniques, have identified quantitatively analyzed distinct topological features shape network wild-type Sema7A loss-of-function mutants. contrast those animals, mutants display aberrant arborizations with disorganized topology diminished contacts Moreover, ectopic expression secreted form by non-hair cells induces chemotropic guidance axons. Our findings demonstrate functions both juxtracrine cue pattern circuitry during organ development.

Язык: Английский

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Semaphorin7A patterns neural circuitry in the lateral line of the zebrafish

Опубликована: Сен. 12, 2023

In a developing nervous system, axonal arbors often undergo complex rearrangements before neural circuits attain their final innervation topology. the lateral line sensory system of zebrafish, axons reorganize terminal arborization patterns to establish precise microcircuits around mechanosensory hair cells. However, quantitative understanding changes in arbor morphology and regulators behind microcircuit assembly remain enigmatic. Here, we report that Semaphorin7A (Sema7A) acts as an important mediator these processes. Utilizing semi-automated three-dimensional neurite tracing methodology computational techniques, have identified quantitatively analyzed distinct topological features shape network wild-type Sema7A loss-of-function mutants. contrast those animals, mutants display aberrant arborizations with disorganized topology diminished contacts Moreover, ectopic expression secreted form by non-hair cells induces chemotropic guidance axons. Our findings demonstrate functions both juxtracrine cue pattern circuitry during organ development.

Язык: Английский

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