Information flow, cell types and stereotypy in a full olfactory connectome

eLife, Год журнала: 2021, Номер 10

Опубликована: Май 25, 2021

The

Язык: Английский

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Chemoreceptor co-expression in Drosophila melanogaster olfactory neurons

eLife, Год журнала: 2022, Номер 11

Опубликована: Апрель 20, 2022

olfactory neurons have long been thought to express only one chemosensory receptor gene family. There are two main families in

Язык: Английский

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The proton channel OTOP1 is a sensor for the taste of ammonium chloride

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Окт. 5, 2023

Abstract Ammonium (NH 4 + ), a breakdown product of amino acids that can be toxic at high levels, is detected by taste systems organisms ranging from C. elegans to humans and has been used for decades in vertebrate research. Here we report OTOP1, proton-selective ion channel expressed sour (Type III) receptor cells (TRCs), functions as sensor ammonium chloride Cl). Extracellular NH Cl evoked large dose-dependent inward currents HEK-293 expressing murine OTOP1 (mOTOP1), human other species variants correlated with its ability alkalinize the cell cytosol. Mutation conserved intracellular arginine residue (R292) mOTOP1 tm 6-tm 7 linker specifically decreased responses relative acid stimuli. Taste measured isolated Type III TRCs, or gustatory nerves were strongly attenuated eliminated an Otop1 −/− mouse strain. Behavioral aversion mice Cl, reduced Skn-1a lacking II was entirely abolished double knockout . These data together reveal unexpected role proton mediating major component previously undescribed activation mechanism.

Язык: Английский

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Aggregation pheromones have a non-linear effect on oviposition behavior in Drosophila melanogaster

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Март 20, 2023

Female fruit flies (Drosophila melanogaster) oviposit at communal sites where the larvae may cooperate or compete for resources depending on group size. This offers a model system to determine how females assess quantitative social information. We show that concentration of pheromones found substrate increases linearly with number adult have visited site. Females prefer oviposition pheromone concentrations corresponding an intermediate previous visitors, whereas low high are unattractive. dose-dependent decision is based blend 11-cis-Vaccenyl Acetate (cVA) indicating visitors and heptanal (a novel deriving from oxidation 7-Tricosene), which acts as dose-independent co-factor. response mediated by detection cVA odorant receptor neurons Or67d Or65a, least five different heptanal. Our results identify mechanism allowing individuals transform linear increase into non-linear behavioral response.

Язык: Английский

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Polymodal sensory perception drives settlement and metamorphosis of Ciona larvae

Current Biology, Год журнала: 2024, Номер 34(6), С. 1168 - 1182.e7

Опубликована: Фев. 8, 2024

The Earth's oceans brim with an incredible diversity of microscopic lifeforms, including motile planktonic larvae, whose survival critically depends on effective dispersal in the water column and subsequent exploration seafloor to identify a suitable settlement site. How their nervous systems mediate sensing diverse multimodal cues remains enigmatic. Here, we uncover that tunicate Ciona intestinalis larvae employ ectodermal sensory cells sense various mechanical chemical cues. Combining whole-brain imaging chemogenetics, demonstrate stimuli encoded at periphery are sufficient drive global brain-state changes promote or impede both larval attachment metamorphosis behaviors. ability C. leverage polymodal perception support information coding chemotactile behaviors may explain how marine make complex decisions despite streamlined systems.

Язык: Английский

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Multilayer regulation underlies the functional precision and evolvability of the olfactory system

bioRxiv (Cold Spring Harbor Laboratory), Год журнала: 2025, Номер unknown

Опубликована: Янв. 16, 2025

Sensory neurons must be reproducibly specified to permit accurate neural representation of external signals but also able change during evolution. We studied this paradox in the Drosophila olfactory system by establishing a single-cell transcriptomic atlas all developing antennal sensory lineages, including latent populations that normally undergo programmed cell death (PCD). This reveals transcriptional control is robust, imperfect, defining selective receptor expression. A second layer precision afforded intersection expression functionally-interacting subunits. third defined stereotyped PCD patterning, which masks promiscuous fated die and removes "empty" lacking receptors. Like choice, under lineage-specific control; promiscuity regulation leads previously-unappreciated heterogeneity neuronal numbers. Thus functional mature belies developmental noise might facilitate evolution pathways.

Язык: Английский

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Fly-antennae-inspired biomass-based fluorescent platform for NH3 quantitative detection and visual real-time monitoring of seafood spoilage

Journal of Hazardous Materials, Год журнала: 2022, Номер 434, С. 128868 - 128868

Опубликована: Апрель 6, 2022

Язык: Английский

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Functional Interaction Between Drosophila Olfactory Sensory Neurons and Their Support Cells

Frontiers in Cellular Neuroscience, Год журнала: 2022, Номер 15

Опубликована: Янв. 7, 2022

Insects detect volatile chemicals using antennae, which house a vast variety of olfactory sensory neurons (OSNs) that innervate hair-like structures called sensilla where odor detection takes place. In addition to OSNs, the antenna also hosts various support cell types. These include triad trichogen, tormogen, and thecogen cells lie adjacent their respective OSNs. The arrangement OSN supporting occurs stereotypically for all is widely conserved in evolution. While insect chemosensory have received considerable attention, little known about functional significance them. For instance, it remains unknown whether play an active role detection, or only passively contribute homeostasis, e.g., by maintaining sensillum lymph composition. To investigate interaction between OSNs cells, we used optical electrophysiological approaches Drosophila. First, characterized distribution genetic markers. By means ex vivo antennal preparation genetically-encoded Ca2+ K+ indicators, then studied activation these auxiliary during presentation adult flies. We observed acute responses distinct differences fluxes Finally, alterations upon ablation mature adults. Upon inducible notice gain mechanical responsiveness stimulations single-sensillum recording, but lack change neuronal resting activity. Taken together, results demonstrate more responsive processing than previously thought. Our observations thus reveal functionally interact with may be important extraordinary ability systems dynamically sensitively discriminate odors turbulent landscape flight.

Язык: Английский

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Systematic morphological and morphometric analysis of identified olfactory receptor neurons in Drosophila melanogaster

eLife, Год журнала: 2021, Номер 10

Опубликована: Авг. 23, 2021

The biophysical properties of sensory neurons are influenced by their morphometric and morphological features, whose precise measurements require high-quality volume electron microscopy (EM). However, systematic surveys nanoscale characteristics for identified scarce. Here, we characterize the morphology Drosophila olfactory receptor (ORNs) across majority genetically hairs. By analyzing serial block-face images cryofixed antennal tissues, compile an extensive data set based on 122 reconstructed 3D models 33 40 ORN types. Additionally, observe multiple novel features-including extracellular vacuoles within sensillum lumen, intricate dendritic branching, mitochondria enrichment in select ORNs, types, empty sensilla containing no neurons-which raise new questions pertinent to cell biology neurobiology. Our survey is critical future investigations into how size shape influence responses, sensitivity, circuit function.

Язык: Английский

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Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons

Frontiers in Cellular Neuroscience, Год журнала: 2021, Номер 15

Опубликована: Ноя. 17, 2021

Two large families of olfactory receptors, the Odorant Receptors (ORs) and Ionotropic (IRs), mediate responses to most odors in insect system. Individual odorant binding “tuning” OrX receptors are expressed by neurons basiconic trichoid sensilla require co-receptor Orco. The situation for IRs is more complex. Different tuning IrX coeloconic rely on either Ir25a or Ir8a co-receptors; some evidence suggests that Ir76b may also act as a co-receptor, but its function has not been systematically examined. Surprisingly, recent data indicate nearly all co-express Ir25a, Ir8a, Ir76b. Here, we demonstrate together amine-sensing receptor neurons. In neurons, loss abolishes amine responses. contrast, persist absence ac1 lost double mutant. We show mediated acid-sensing do Ir76b, despite their expression this co-receptor. Our study demonstrates one population exhibits Ir76b/Ir25a-dependent Orco-dependent distinct odorants. Together, our establish role bona fide which acts partnership with Ir25a. Given these co-receptors among highly conserved often co-expressed chemosensory suggest work tandem other insects.

Язык: Английский

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