Environmental DNA methods for biosecurity and invasion biology in terrestrial ecosystems: Progress, pitfalls, and prospects

The Science of The Total Environment, Год журнала: 2024, Номер 926, С. 171810 - 171810

Опубликована: Март 19, 2024

Язык: Английский

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Continuous daily sampling of airborne eDNA detects all vertebrate species identified by camera traps

Environmental DNA, Год журнала: 2024, Номер 6(4)

Опубликована: Июль 1, 2024

Abstract Ongoing pressures on global biodiversity require conservation action that is not possible without effective biomonitoring. Terrestrial vertebrate surveys are commonly performed using camera traps, a time‐intensive method known to miss many small or arboreal species and birds. Recent advances have shown airborne eDNA be potentially suitable technique more effectively monitor communities in time‐ cost‐effective manner. Here, we test whether commercially available air samplers collect particles 24/7 during 1‐week period can used detect the presence of vertebrates through eDNA. The results compared trap records at three locations with differing habitats Netherlands. Simultaneous sampling different for 3 weeks resulted detection 154 taxa, which majority were birds mammals (113 33 species, respectively), along four fish amphibian species. All observed traps also retrieved via eDNA, although every day sampling. Burkard spore trap, routinely pollen monitoring, showed highest number only samples when mammal was detected it remained undetected We unique indicative habitat they living. However, could account for. multitude found indicate sensitivity method; however, subsequent studies should prioritize validation these findings alternative biomonitoring approaches.

Язык: Английский

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A review of eDNA technology in avian monitoring: Current status, challenges and future perspectives

Avian Research, Год журнала: 2025, Номер unknown, С. 100235 - 100235

Опубликована: Март 1, 2025

Язык: Английский

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A review of applications of environmental DNA for reptile conservation and management

Ecology and Evolution, Год журнала: 2022, Номер 12(6)

Опубликована: Июнь 1, 2022

Abstract Reptile populations are in decline globally, with total reptile abundance halving the past half century, and approximately a fifth of species currently threatened extinction. Research on distributions, population trends, trophic interactions can greatly improve accuracy conservation listings planning for recovery, but data deficiency is an impediment many species. Environmental DNA (eDNA) detect measure community diversity at diverse spatio‐temporal scales, especially useful detection elusive, cryptic, or rare species, making it potentially very valuable herpetology. We aim to summarize utility eDNA as tool informing management discuss benefits limitations this approach. A literature review was conducted collect all studies that used focus ecology, conservation, management. Results search summarized into key discussion points, also draws from other taxa highlight methodological challenges identify future research directions. has had limited application reptiles, relative vertebrate groups, little use regions high richness. techniques have been more successfully applied aquatic reptiles than terrestrial most (64%) focused habitats. Two four reptilian orders dominate existing (56% Testudines, 49% Squamata, 5% Crocodilia, 0% Rhynchocephalia). Our provides direction emerging ecology when be paired traditional monitoring approaches. Technologies associated rapidly advancing, become sensitive accessible, we expect will increasingly addressing knowledge gaps reptiles.

Язык: Английский

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Transforming terrestrial biodiversity surveys using airborne eDNA

Trends in Ecology & Evolution, Год журнала: 2022, Номер 38(2), С. 119 - 121

Опубликована: Дек. 10, 2022

Studies show that land-living animals, plants, fungi, and bacteria leave DNA traces in the air. These results imply sequencing of bioaerosols might be a powerful tool for simultaneous surveys terrestrial biodiversity across lifeforms, but parallel, it highlights need to carefully control possible contaminants.

Язык: Английский

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Accumulation and diversity of airborne, eukaryotic environmental DNA

Environmental DNA, Год журнала: 2022, Номер 4(6), С. 1323 - 1339

Опубликована: Июль 18, 2022

Abstract Environmental DNA (eDNA) metabarcoding is increasingly being implemented as a non‐invasive and efficient approach for biodiversity research monitoring across ecosystems. However, accurate detection of species with eDNA requires robust experimental designs analysis carries risk contamination at every step the fieldwork laboratory processes. Several studies focus on rigorous procedures processing sequencing data, but surprisingly, little investigates process background input in field. For example, airborne from localities outside study area could potentially contaminate samples. Here, we use an setup to diversity accumulation eukaryotic exposed surfaces At two different natural locations, coastal marine site terrestrial grassland site, placed open containers each filled 0.5 liters water, which was then sampled eight successive time points after exposure surroundings. We found detected richness samples, reached its maximum end experiment, 24 h exposure. This result consistent both sites markers (COI eukaryotes 12S vertebrates). While many were contaminants commonly studies, also several other taxa. Most notable metazoan such birds, fish, insects, likely originating transport eDNA. that increasing number PCR cycles tended have positive impact unfiltered reads negative bioinformatic filtering. Our results add sparse evidence can be transported by air, wide implications calls increased implementation field control

Язык: Английский

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A manager’s guide to using eDNA metabarcoding in marine ecosystems

PeerJ, Год журнала: 2022, Номер 10, С. e14071 - e14071

Опубликована: Ноя. 15, 2022

Environmental DNA (eDNA) metabarcoding is a powerful tool that can enhance marine ecosystem/biodiversity monitoring programs. Here we outline five important steps managers and researchers should consider when developing eDNA program: (1) select genes primers to target taxa; (2) assemble or develop comprehensive barcode reference databases; (3) apply rigorous site occupancy based decontamination pipelines; (4) conduct pilot studies define spatial temporal variance of eDNA; (5) archive samples, extracts, raw sequence data. We demonstrate the importance each these considerations using case study in Ports Los Angeles Long Beach. approaches detected 94.1% (16/17) species observed paired trawl surveys while identifying an additional 55 native fishes, providing more biodiversity inventories. Rigorous benchmarking results improved ecological interpretation confidence detections archived genetic resources for future analyses. Well designed validated are ideally suited biomonitoring applications rely on detection species, including mapping invasive fronts endangered habitats as well tracking range shifts response climate change. Incorporating will utility efficacy routine applications.

Язык: Английский

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Airborne eDNA documents a diverse and ecologically complex tropical bat and other mammal community

Environmental DNA, Год журнала: 2023, Номер 5(2), С. 350 - 362

Опубликована: Янв. 11, 2023

Abstract Environmental (e)DNA has rapidly become a powerful biomonitoring tool, particularly in aquatic ecosystems. This approach not been as widely adopted terrestrial communities where the methods of vertebrate eDNA collection have varied from use secondary collectors such blood feeding parasites and spider webs, to washing surfaces leaves soil sampling. Recent studies demonstrated potential direct air sampling, but none tested how effective airborne sampling might be biodiverse environment. We used three prototype samplers actively sample mixed neotropical bat community partially controlled assess whether can accurately characterize high diversity with skewed abundances determine if filter design impacts DNA taxonomic recovery. Our study provides evidence for accuracy detection tool highlights its monitoring density, diverse assemblages roosts. Analysis samples recovered >91% species present some limited relationship between abundance read count. data suggests this method depict mixed‐mammal community, when location is contained (e.g., roost, den or burrow) also transfer material on clothing equipment. results demonstrate that simple, inexpensive, battery‐operated homemade collect an air, opening opportunity remote environments.

Язык: Английский

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Spider webs capture environmental DNA from terrestrial vertebrates

iScience, Год журнала: 2024, Номер 27(2), С. 108904 - 108904

Опубликована: Янв. 30, 2024

Environmental DNA holds significant promise as a non-invasive tool for tracking terrestrial biodiversity. However, in non-homogenous environments, the continual exploration of new substrates is crucial. Here we test hypothesis that spider webs can act passive biofilters, capturing eDNA from vertebrates present local environment. Using metabarcoding approach, detected vertebrate all analyzed (N = 49). Spider obtained an Australian woodland locality yielded 32 different species, including native mammals and birds. In contrast, Perth Zoo, less than 50 km away, 61 produced highly distinct species composition, largely reflecting exotic hosted zoo. We show higher animal biomass proximity to enclosures increased detection probability Our results indicate tremendous potential using cost-effective means monitor vertebrates.

Язык: Английский

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Emerging use of air eDNA and its application to forensic investigations – A review

Electrophoresis, Год журнала: 2024, Номер 45(9-10), С. 916 - 932

Опубликована: Фев. 28, 2024

Abstract Biological material is routinely collected at crime scenes and from exhibits a key type of evidence during criminal investigations. Improvements in DNA technologies allow collection profiling trace samples, comprised few cells, significantly expanding the types targeted for analysis to include touched surfaces. However, success rates touch samples tend be poorer compared other biological materials such as blood. Simultaneously, there have been recent advances utility environmental (eDNA) identification tracking different organisms species bacteria naked mole rats environments, including, soil, ice, snow, air aquatic. This paper examines emerging methods research into eDNA collection, with special emphasis on potential forensic applications human including challenges further studies required progress implementation.

Язык: Английский

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