Environmental DNA: The next chapter

Molecular Ecology, Год журнала: 2024, Номер 33(11)

Опубликована: Апрель 16, 2024

Abstract Molecular tools are an indispensable part of ecology and biodiversity sciences implemented across all biomes. About a decade ago, the use implementation environmental DNA (eDNA) to detect signals extracted from samples opened new avenues research. Initial eDNA research focused on understanding population dynamics target species. Its scope thereafter broadened, uncovering previously unrecorded via metabarcoding in both well‐studied understudied ecosystems taxonomic groups. The application rapidly became established research, field by its own. Here, we revisit key expectations made land‐mark special issue Ecology 2012 frame development six areas: (1) sample collection, (2) primer development, (3) biomonitoring, (4) quantification, (5) behaviour environment (6) reference database development. We pinpoint success eDNA, yet also discuss shortfalls not met, highlighting areas priority identify unexpected developments. In parallel, our retrospective couples screening peer‐reviewed literature with survey users including academics, end‐users commercial providers, which address focus efforts advance eDNA. With rapid ever‐increasing pace technical advances, future looks bright, successful applications best practices must become more interdisciplinary reach full potential. Our retrospect gives towards concretely moving forward.

Язык: Английский

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Realising a global One Health disease surveillance approach: insights from wastewater and beyond

Nature Communications, Год журнала: 2024, Номер 15(1)

Опубликована: Июнь 22, 2024

Abstract One Health is a recognition of the shared environment inhabited by humans, animals and plants, impact their interactions on health all organisms. The COVID-19 pandemic highlighted need for framework pathogen surveillance in tractable paradigm to allow timely detection response threats human animal health. We present case studies centered around recent global approach tackle antimicrobial resistance current interest wastewater testing, with concept “one sample many analyses” be further explored as most appropriate means initiating this endeavor.

Язык: Английский

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Targeting Terrestrial Vertebrates With eDNA: Trends, Perspectives, and Considerations for Sampling

Environmental DNA, Год журнала: 2025, Номер 7(1)

Опубликована: Янв. 1, 2025

ABSTRACT Terrestrial vertebrates are experiencing worldwide population declines and species extinctions. To effectively conserve remaining populations species, rapid, cost‐effective, scalable methods needed to complement longstanding monitoring methods. Increasingly, environmental DNA (eDNA)‐based approaches being used for terrestrial vertebrate biomonitoring within a range of environments. However, as we move eDNA onto land, presented with new set challenges. This necessitates the development “best‐practice” sample collection guidelines systems purpose detecting vertebrates. address these needs, conducted systematic literature review 143 peer‐reviewed papers applying (excluding Lissamphibia) that were published between 2012 2023. We summarize use biomonitoring, focusing on study design field techniques. Over decade observe steady growth in annual number publications, 3 33 The majority reviewed studies targeted mammals temperate forest regions. While an equal focused metabarcoding approach assess community taxon composition and/or species‐specific detection methods, novel uses increasingly published. These include animal behavior genetics. record three types sampling strategies, eight different substrate types, seven preservation suggesting there is no “one size fits all” eDNA‐based methodology when With multitude aims, across environments, target organisms ecologies, standardization extremely challenging. table known factors influencing Furthermore, identify five key considerations be addressed targeting aim guiding decision making.

Язык: Английский

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Continuous daily sampling of airborne eDNA detects all vertebrate species identified by camera traps

Environmental DNA, Год журнала: 2024, Номер 6(4)

Опубликована: Июль 1, 2024

Abstract Ongoing pressures on global biodiversity require conservation action that is not possible without effective biomonitoring. Terrestrial vertebrate surveys are commonly performed using camera traps, a time‐intensive method known to miss many small or arboreal species and birds. Recent advances have shown airborne eDNA be potentially suitable technique more effectively monitor communities in time‐ cost‐effective manner. Here, we test whether commercially available air samplers collect particles 24/7 during 1‐week period can used detect the presence of vertebrates through eDNA. The results compared trap records at three locations with differing habitats Netherlands. Simultaneous sampling different for 3 weeks resulted detection 154 taxa, which majority were birds mammals (113 33 species, respectively), along four fish amphibian species. All observed traps also retrieved via eDNA, although every day sampling. Burkard spore trap, routinely pollen monitoring, showed highest number only samples when mammal was detected it remained undetected We unique indicative habitat they living. However, could account for. multitude found indicate sensitivity method; however, subsequent studies should prioritize validation these findings alternative biomonitoring approaches.

Язык: Английский

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Emerging technologies for pollinator monitoring

Current Opinion in Insect Science, Год журнала: 2025, Номер unknown, С. 101367 - 101367

Опубликована: Март 1, 2025

Язык: Английский

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Conservation tools: the next generation of engineering–biology collaborations

Journal of The Royal Society Interface, Год журнала: 2023, Номер 20(205)

Опубликована: Авг. 1, 2023

The recent increase in public and academic interest preserving biodiversity has led to the growth of field conservation technology. This involves designing constructing tools that use technology aid wildlife. In this review, we present five case studies infer a framework for (CT) based on human–wildlife interaction. Successful CT range complexity from cat collars machine learning game theory methodologies do not require technological expertise contribute tool creation. Our goal is introduce researchers provide references guiding next generation technologists. Conservation only potential benefit but also broader impacts fields such as sustainability environmental protection. By using innovative technologies address challenges, can find more effective efficient solutions protect preserve our planet’s resources.

Язык: Английский

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eDNA for monitoring and conserving terrestrial arthropods: Insights from a systematic map and barcode repositories assessments

Insect Conservation and Diversity, Год журнала: 2024, Номер 17(4), С. 565 - 578

Опубликована: Март 8, 2024

Abstract In the past decade, environmental DNA (eDNA) assays have become an essential tool to investigate species presence with samples from environment instead of collected specimens. eDNA sampling techniques proved their worth in freshwater and marine studies; now, some trends emerge for use terrestrial habitats particularly study arthropods. After a systematic review literature, we illustrate analyse diversity such studies discuss benefits drawbacks. We identified most relevant research themes focused on (i) taxa sample types targeted (ii) details survey scheme. parallel, also assessed available number sequences cytochrome c oxidase subunit I (COI), 16S 18S barcode regions four major (spiders, centipedes, springtails insects) relation diversity. found strong taxonomic geographic biases regarding coverage per barcode. arthropods mainly focuses insect that affect humanity positive or negative way, availability is much higher temperate‐developed countries than tropical ones. Moreover, although high variety are being used, do not assess completeness target nor compare efficacy monitoring technique other well established known traditional techniques. Careful workflow designs comparisons needed before giving any management conservation advice as does come without error. Strengths weaknesses discussed.

Язык: Английский

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Capturing the Aerobiome: Application of Polyurethane Foam Disk Passive Samplers for Bioaerosol Monitoring

ACS ES&T Air, Год журнала: 2024, Номер 1(5), С. 414 - 425

Опубликована: Март 21, 2024

Bioaerosols are ubiquitous and play a significant role in global climate human health due to inhalation exposure. Passive air sampling of bioaerosols, as complementary method active using pumps, is increasingly valued its simplicity, electricity-free operations, cost-effectiveness providing time-integrated samples over weeks/months. In this study, polyurethane foam disk passive samplers (PUF-PAS), dry deposition (PAS-DDs), high-volume (Hi-Vol) were deployed Toronto the Athabasca oil sands region (OSR) first stages proof-of-concept exercise for bioaerosols. Airborne bacterial fungal communities characterized MiSeq DNA sequencing. All sampler types shown successfully collect The dominant phyla observed by all qualitatively similar. Species richness community structure airborne varied with sites seasons. Principal coordination analysis indicated that differed between OSR. Further work required calibrate characterize uptake PUF-PAS PAS-DD bioaerosols derive quantitative information on their abundance better assess sources potential exposure risks.

Язык: Английский

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Environmental DNA without borders

EMBO Reports, Год журнала: 2024, Номер 25(10), С. 4095 - 4099

Опубликована: Сен. 25, 2024

Язык: Английский

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European pollen reanalysis, 1980–2022, for alder, birch, and olive

Scientific Data, Год журнала: 2024, Номер 11(1)

Опубликована: Окт. 3, 2024

Язык: Английский

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