bioRxiv (Cold Spring Harbor Laboratory),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Dec. 11, 2023
Abstract
Conserving
biodiversity
is
a
global
imperative,
yet
our
capacity
to
quantify
and
understand
species
occurrences
has
been
limited.
To
help
address
this
challenge,
we
develop
novel
monitoring
approach
based
on
deep
sequencing
of
airborne
eDNA.
When
applied
34-year
archive
weekly
filters
from
an
aerosol
sampling
station
in
northern
Sweden,
methods
enabled
robust
detection
over
2,700
genera
across
all
domains
life
estimates
eDNA
catchment
areas.
Reconstructed
time
series
revealed
regional
declines
consistent
with
contemporary,
large-scale
transformations
forest
composition
structure.
Our
results
show
can
reliably
monitor
underscore
the
immense
latent
potential
thousands
stations
deployed
worldwide.
One-Sentence
Summary
DNA
captured
air
reveals
organisms
their
long-term
trends.
Molecular Ecology,
Journal Year:
2024,
Volume and Issue:
33(11)
Published: April 16, 2024
Abstract
Molecular
tools
are
an
indispensable
part
of
ecology
and
biodiversity
sciences
implemented
across
all
biomes.
About
a
decade
ago,
the
use
implementation
environmental
DNA
(eDNA)
to
detect
signals
extracted
from
samples
opened
new
avenues
research.
Initial
eDNA
research
focused
on
understanding
population
dynamics
target
species.
Its
scope
thereafter
broadened,
uncovering
previously
unrecorded
via
metabarcoding
in
both
well‐studied
understudied
ecosystems
taxonomic
groups.
The
application
rapidly
became
established
research,
field
by
its
own.
Here,
we
revisit
key
expectations
made
land‐mark
special
issue
Ecology
2012
frame
development
six
areas:
(1)
sample
collection,
(2)
primer
development,
(3)
biomonitoring,
(4)
quantification,
(5)
behaviour
environment
(6)
reference
database
development.
We
pinpoint
success
eDNA,
yet
also
discuss
shortfalls
not
met,
highlighting
areas
priority
identify
unexpected
developments.
In
parallel,
our
retrospective
couples
screening
peer‐reviewed
literature
with
survey
users
including
academics,
end‐users
commercial
providers,
which
address
focus
efforts
advance
eDNA.
With
rapid
ever‐increasing
pace
technical
advances,
future
looks
bright,
successful
applications
best
practices
must
become
more
interdisciplinary
reach
full
potential.
Our
retrospect
gives
towards
concretely
moving
forward.
Nature Communications,
Journal Year:
2024,
Volume and Issue:
15(1)
Published: June 22, 2024
Abstract
One
Health
is
a
recognition
of
the
shared
environment
inhabited
by
humans,
animals
and
plants,
impact
their
interactions
on
health
all
organisms.
The
COVID-19
pandemic
highlighted
need
for
framework
pathogen
surveillance
in
tractable
paradigm
to
allow
timely
detection
response
threats
human
animal
health.
We
present
case
studies
centered
around
recent
global
approach
tackle
antimicrobial
resistance
current
interest
wastewater
testing,
with
concept
“one
sample
many
analyses”
be
further
explored
as
most
appropriate
means
initiating
this
endeavor.
Environmental DNA,
Journal Year:
2025,
Volume and Issue:
7(1)
Published: Jan. 1, 2025
ABSTRACT
Terrestrial
vertebrates
are
experiencing
worldwide
population
declines
and
species
extinctions.
To
effectively
conserve
remaining
populations
species,
rapid,
cost‐effective,
scalable
methods
needed
to
complement
longstanding
monitoring
methods.
Increasingly,
environmental
DNA
(eDNA)‐based
approaches
being
used
for
terrestrial
vertebrate
biomonitoring
within
a
range
of
environments.
However,
as
we
move
eDNA
onto
land,
presented
with
new
set
challenges.
This
necessitates
the
development
“best‐practice”
sample
collection
guidelines
systems
purpose
detecting
vertebrates.
address
these
needs,
conducted
systematic
literature
review
143
peer‐reviewed
papers
applying
(excluding
Lissamphibia)
that
were
published
between
2012
2023.
We
summarize
use
biomonitoring,
focusing
on
study
design
field
techniques.
Over
decade
observe
steady
growth
in
annual
number
publications,
3
33
The
majority
reviewed
studies
targeted
mammals
temperate
forest
regions.
While
an
equal
focused
metabarcoding
approach
assess
community
taxon
composition
and/or
species‐specific
detection
methods,
novel
uses
increasingly
published.
These
include
animal
behavior
genetics.
record
three
types
sampling
strategies,
eight
different
substrate
types,
seven
preservation
suggesting
there
is
no
“one
size
fits
all”
eDNA‐based
methodology
when
With
multitude
aims,
across
environments,
target
organisms
ecologies,
standardization
extremely
challenging.
table
known
factors
influencing
Furthermore,
identify
five
key
considerations
be
addressed
targeting
aim
guiding
decision
making.
Environmental DNA,
Journal Year:
2024,
Volume and Issue:
6(4)
Published: July 1, 2024
Abstract
Ongoing
pressures
on
global
biodiversity
require
conservation
action
that
is
not
possible
without
effective
biomonitoring.
Terrestrial
vertebrate
surveys
are
commonly
performed
using
camera
traps,
a
time‐intensive
method
known
to
miss
many
small
or
arboreal
species
and
birds.
Recent
advances
have
shown
airborne
eDNA
be
potentially
suitable
technique
more
effectively
monitor
communities
in
time‐
cost‐effective
manner.
Here,
we
test
whether
commercially
available
air
samplers
collect
particles
24/7
during
1‐week
period
can
used
detect
the
presence
of
vertebrates
through
eDNA.
The
results
compared
trap
records
at
three
locations
with
differing
habitats
Netherlands.
Simultaneous
sampling
different
for
3
weeks
resulted
detection
154
taxa,
which
majority
were
birds
mammals
(113
33
species,
respectively),
along
four
fish
amphibian
species.
All
observed
traps
also
retrieved
via
eDNA,
although
every
day
sampling.
Burkard
spore
trap,
routinely
pollen
monitoring,
showed
highest
number
only
samples
when
mammal
was
detected
it
remained
undetected
We
unique
indicative
habitat
they
living.
However,
could
account
for.
multitude
found
indicate
sensitivity
method;
however,
subsequent
studies
should
prioritize
validation
these
findings
alternative
biomonitoring
approaches.
Journal of The Royal Society Interface,
Journal Year:
2023,
Volume and Issue:
20(205)
Published: Aug. 1, 2023
The
recent
increase
in
public
and
academic
interest
preserving
biodiversity
has
led
to
the
growth
of
field
conservation
technology.
This
involves
designing
constructing
tools
that
use
technology
aid
wildlife.
In
this
review,
we
present
five
case
studies
infer
a
framework
for
(CT)
based
on
human–wildlife
interaction.
Successful
CT
range
complexity
from
cat
collars
machine
learning
game
theory
methodologies
do
not
require
technological
expertise
contribute
tool
creation.
Our
goal
is
introduce
researchers
provide
references
guiding
next
generation
technologists.
Conservation
only
potential
benefit
but
also
broader
impacts
fields
such
as
sustainability
environmental
protection.
By
using
innovative
technologies
address
challenges,
can
find
more
effective
efficient
solutions
protect
preserve
our
planet’s
resources.
Insect Conservation and Diversity,
Journal Year:
2024,
Volume and Issue:
17(4), P. 565 - 578
Published: March 8, 2024
Abstract
In
the
past
decade,
environmental
DNA
(eDNA)
assays
have
become
an
essential
tool
to
investigate
species
presence
with
samples
from
environment
instead
of
collected
specimens.
eDNA
sampling
techniques
proved
their
worth
in
freshwater
and
marine
studies;
now,
some
trends
emerge
for
use
terrestrial
habitats
particularly
study
arthropods.
After
a
systematic
review
literature,
we
illustrate
analyse
diversity
such
studies
discuss
benefits
drawbacks.
We
identified
most
relevant
research
themes
focused
on
(i)
taxa
sample
types
targeted
(ii)
details
survey
scheme.
parallel,
also
assessed
available
number
sequences
cytochrome
c
oxidase
subunit
I
(COI),
16S
18S
barcode
regions
four
major
(spiders,
centipedes,
springtails
insects)
relation
diversity.
found
strong
taxonomic
geographic
biases
regarding
coverage
per
barcode.
arthropods
mainly
focuses
insect
that
affect
humanity
positive
or
negative
way,
availability
is
much
higher
temperate‐developed
countries
than
tropical
ones.
Moreover,
although
high
variety
are
being
used,
do
not
assess
completeness
target
nor
compare
efficacy
monitoring
technique
other
well
established
known
traditional
techniques.
Careful
workflow
designs
comparisons
needed
before
giving
any
management
conservation
advice
as
does
come
without
error.
Strengths
weaknesses
discussed.
ACS ES&T Air,
Journal Year:
2024,
Volume and Issue:
1(5), P. 414 - 425
Published: March 21, 2024
Bioaerosols
are
ubiquitous
and
play
a
significant
role
in
global
climate
human
health
due
to
inhalation
exposure.
Passive
air
sampling
of
bioaerosols,
as
complementary
method
active
using
pumps,
is
increasingly
valued
its
simplicity,
electricity-free
operations,
cost-effectiveness
providing
time-integrated
samples
over
weeks/months.
In
this
study,
polyurethane
foam
disk
passive
samplers
(PUF-PAS),
dry
deposition
(PAS-DDs),
high-volume
(Hi-Vol)
were
deployed
Toronto
the
Athabasca
oil
sands
region
(OSR)
first
stages
proof-of-concept
exercise
for
bioaerosols.
Airborne
bacterial
fungal
communities
characterized
MiSeq
DNA
sequencing.
All
sampler
types
shown
successfully
collect
The
dominant
phyla
observed
by
all
qualitatively
similar.
Species
richness
community
structure
airborne
varied
with
sites
seasons.
Principal
coordination
analysis
indicated
that
differed
between
OSR.
Further
work
required
calibrate
characterize
uptake
PUF-PAS
PAS-DD
bioaerosols
derive
quantitative
information
on
their
abundance
better
assess
sources
potential
exposure
risks.
