ATP and glutamate coordinate contractions in the freshwater sponge Ephydatia muelleri

Journal of Experimental Biology, Год журнала: 2025, Номер 228(3)

Опубликована: Фев. 1, 2025

ABSTRACT Sponges (phylum Porifera) are an early diverging animal lineage without nervous and muscular systems, yet they able to produce coordinated whole-body contractions in response disturbances. Little is known about the underlying signalling mechanisms coordinating such responses. Previous studies demonstrated that sponges respond specifically chemicals as l-glutamate γ-amino-butyric acid (GABA), which trigger prevent contractions, respectively. Genes for purinergic P2X-like receptors present several sponge genomes, leading us ask whether ATP works with glutamate coordinate it does other systems. Using pharmacological approaches on freshwater Ephydatia muelleri, we show involved contractions. Bath application of caused a rapid, sustained expansion excurrent canals dose-dependent manner. Complete occurred when was added presence apyrase, enzyme hydrolyses ATP. Application ADP, first metabolic product hydrolysis, triggered complete whereas AMP, subsequent metabolite, did not response. Blocking from binding activating P2X pyridoxalphosphate-6-azophenyl-2′,4′-disulfonic (PPADS) prevented both glutamate- ATP-triggered suggesting downstream glutamate. Bioinformatic analysis revealed two receptor sequences, one groups vertebrate receptors. Altogether, our results confirm by sponge.

Язык: Английский

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A morphological cell atlas of the freshwater sponge Ephydatia muelleri with key insights from targeted single-cell transcriptomes

EvoDevo, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 14, 2025

How animal cell types, tissues, and regional body plans arose is a fundamental question in EvoDevo. Many current efforts attempt to link genetic information the morphology of cells, tissues regionalization using single-cell sequencing populations. However, lack in-depth understanding non-bilaterian animals remains considerable block transitions between bilaterian cells tissues. Sponges (Porifera), one earliest diverging phyla, pose particular challenge this endeavour, because their mouths, gut, conventional muscle nervous systems. With goal help bridge gap, we have studied morphology, behaviour transcriptomics tissue types an easily accessible well-studied species freshwater sponge, Ephydatia muelleri. New features described here include: polarized external epithelium, new contractile sieve that forms entry incurrent canals, motile cilia on apopyle at exit choanocyte chambers, non-motile excurrent canals oscula. Imaging vivo shows distinct behavioural characteristics mesohyl. Transcriptomic phenotypes three (cystencytes, choanocytes archaeocytes) captured live indicate cell-type transcriptomes are distinct. Importantly, individual archaeocytes show range transcriptomic which supported by expression different genes subsets type. In contrast, all five sampled revealed highly uniform with significantly fewer expressed than other types. Our study sponges whose diversity both functionally complex, but together enable like metazoans, sense respond stimuli.

Язык: Английский

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Microtubule organization and tubulin post-translational modifications in intact tissues and during regeneration in calcareous sponges

Cell and Tissue Research, Год журнала: 2025, Номер unknown

Опубликована: Март 5, 2025

Язык: Английский

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Dynamics, diversity, and roles of bacterial transmission modes during the first asexual life stages of the freshwater sponge Spongilla lacustris

Environmental Microbiome, Год журнала: 2024, Номер 19(1)

Опубликована: Июнь 8, 2024

Sponge-associated bacteria play important roles in the physiology of their host, whose recruitment processes are crucial to maintain symbiotic associations. However, acquisition bacterial communities within freshwater sponges is still under explored. Spongilla lacustris a model sponge widely distributed European rivers and lakes, producing dormant cysts (named gemmules) for asexual reproduction, before winter. Through an vitro experiment, this study aims describe dynamics transmission modes following hatching these gemmules.

Язык: Английский

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Chemical cognition: chemoconnectomics and convergent evolution of integrative systems in animals

Animal Cognition, Год журнала: 2023, Номер 26(6), С. 1851 - 1864

Опубликована: Ноя. 1, 2023

Язык: Английский

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Particle loads, contractile responses and cleaning in the demosponge Halichondria panicea

Journal of Experimental Marine Biology and Ecology, Год журнала: 2024, Номер 577, С. 152021 - 152021

Опубликована: Май 23, 2024

Sponges exposed to high concentrations of particles during filter-feeding may become overloaded, triggering closure the osculum and contraction sponge, thus preventing too many from entering aquiferous system. However, minimum particle concentration small versus larger that triggers contractions has not yet been described. Here, based on microscope-video recordings, we report our observations explants demosponge Halichondria panicea. Explants contracted within <1 h in response cyanobacteria (Cyanobium bacillare; 1 × 106 cells mL−1), 2 μm beads (1 107 algal (Rhodomonas salina), 10 105 mL−1, respectively). No occurred sponge these types at <0.5 104 mL−1. Likewise, inorganic marl ink induced ranging When were subsequently transferred clean water, they either expelled ingested mucus-entangled clumps with exhalant jet, or translocated surface along local peristaltic-like contractions. The trigger levels compared reported re-suspended sediment sea, observed contractile responses primarily serve protect sponges clogging extreme environmental events.

Язык: Английский

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Adaptive Cellular Radiations and the Genetic Mechanisms Underlying Animal Nervous System Diversification

Annual Review of Cell and Developmental Biology, Год журнала: 2024, Номер 40(1), С. 407 - 425

Опубликована: Июль 25, 2024

In animals, the nervous system evolved as primary interface between multicellular organisms and environment. As became larger more complex, functions of expanded to include modulation coordination individual responsive cells via paracrine synaptic well monitor maintain organism's own internal This was initially accomplished signaling eventually through assembly multicell circuits in some lineages. Cells with similar centralized systems have independently arisen several We highlight molecular mechanisms that underlie parallel diversifications system.

Язык: Английский

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Novel imaging and biophysical approaches to study tissue hydraulics in mammalian folliculogenesis

Biophysical Reviews, Год журнала: 2024, Номер 16(5), С. 625 - 637

Опубликована: Окт. 1, 2024

Abstract A key developmental stage in mammalian folliculogenesis is the formation of a fluid-filled lumen (antrum) prior to ovulation. While it has long been speculated that follicular fluid essential for oocyte maturation and ovulation, little known about morphogenesis mechanisms driving antrum potentially due challenges imaging tissue dynamics large tissues. Misregulation such processes leads anovulation, hallmark infertility ageing diseases as polycystic ovary syndrome (PCOS). In this review, we discuss recent advances deep techniques, machine learning theoretical approaches have applied study development diseases. We propose an integrative approach combining these techniques understanding physics hydraulics follicle ovarian functions.

Язык: Английский

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Animal granulins: In the GRN scheme of things

Developmental & Comparative Immunology, Год журнала: 2023, Номер 152, С. 105115 - 105115

Опубликована: Дек. 13, 2023

Язык: Английский

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Dynamics, diversity, and roles of bacterial transmission modes during the first asexual life stages of the freshwater sponge Spongilla lacustris

Research Square (Research Square), Год журнала: 2024, Номер unknown

Опубликована: Фев. 23, 2024

Background: Sponges-associated bacteria play important roles in the physiology of their host, whose recruitment processes are crucial to maintain symbiotic associations. However, acquisition bacterial communities within freshwater sponges is still unexplored. Spongilla lacustris a model sponge widely distributed European rivers and lakes, producing dormant cysts (named gemmules) for asexual reproduction, before winter. Through an in vitroexperiment, this study aims describe dynamics transmission modes following hatching these gemmules. Results: An overall change β-diversity was observed through ontology new juvenile sponges. These temporal differences were potentially linked first osculum development canal system, then, increasing colonization Chlorella-like photosymbionts. Gemmules with sterilized surface found have more dispersed less diverse microbiome, revealing importance gemmule epibacteria whole holobiont stability. suggested be vertically transmitted from maternal tissues surface. Vertical incorporation inside gemmule, also as dominant mode, especially nitrogen fixers Terasakiellaceae. Finally, we showed that almost no ASVs shared between free-living community juveniles, suggesting horizontal unlikely happen during stages development. filtered probably used source nutrients, allowing enrichment copiotrophic already present its microbiome. Conclusions: This brings insight better understanding microbiome We epibacterial on gemmules stability, demonstrated near absence stages.

Язык: Английский

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