Microorganisms,
Год журнала:
2023,
Номер
11(8), С. 2119 - 2119
Опубликована: Авг. 20, 2023
Cationic
antimicrobial
peptides
are
molecules
with
potential
applications
for
treating
infections
due
to
their
and
immunomodulatory
properties.
The
aim
of
this
work
was
explore
the
activity
mechanisms
action
a
porcine
neutrophil
cathelicidin
mixture
(MPPN).
Gram-positive
Gram-negative
bacteria
were
used
determine
minimum
inhibitory
concentration
(MIC)
experiments
both
time–kill
kinetics
effects
on
growth
curves
performed.
Planar
black
lipid
bilayer
conductance
measured
analyze
interaction
MPPN
bilayers.
Visualization
bacterial
surfaces
membrane
alterations
achieved
using
atomic
force
microscopy
transmission
electron
microscopy.
efflux
pumps
(EPs)
studied
an
intracellular
accumulation
acridine
orange
(AO)
assay.
In
E.
coli,
behaves
as
bactericide
at
high
concentrations
bacteriostatic
lower
concentrations.
effect
also
observed
slightly
shorter
periods
in
S.
enterica.
not
active
aureus.
increase
AO
presence
indicates
that,
least
causes
inhibition
EP
function.
Observed
detected
variable
events
demonstrate
strong
Damage
structure
treated
coli
that
induces
surface.
use
AMPs
capable
inhibiting
can
be
seen
good
tool
combat
resistance
since
they
could
alone
or
combination
other
conventional
antibiotics
which
have
become
resistant.
Trends in biotechnology,
Год журнала:
2025,
Номер
unknown
Опубликована: Март 1, 2025
Novel
antibiotics
are
urgently
needed
since
bacteria
becoming
increasingly
resistant
to
existing
antimicrobial
drugs.
Furthermore,
available
broad
spectrum,
often
causing
off-target
effects
on
host
cells
and
the
beneficial
microbiome.
To
overcome
these
limitations,
we
used
structure-guided
design
generate
synthetic
peptides
derived
from
Andersonin-D1,
an
peptide
(AMP)
produced
by
odorous
frog
Odorrana
andersonii.
We
found
that
both
hydrophobicity
net
charge
were
critical
for
its
bioactivity,
enabling
of
novel,
optimized
peptides.
These
selectively
targeted
Gram-negative
pathogens
in
single
cultures
complex
microbial
consortia,
showed
no
human
or
gut
microbes,
did
not
select
bacterial
resistance.
Notably,
they
also
exhibited
vivo
activity
two
preclinical
murine
models.
Overall,
present
target
pathogenic
infections
offer
promising
antibiotic
candidates.
Journal of Peptide Science,
Год журнала:
2023,
Номер
30(1)
Опубликована: Июль 28, 2023
The
recent
pandemic
caused
by
severe
acute
respiratory
syndrome
coronavirus
2
(SARS‐CoV‐2)
has
highlighted
how
urgent
and
necessary
the
discovery
of
new
antiviral
compounds
is
for
novel
therapeutic
approaches.
Among
various
classes
molecules
with
activity,
antimicrobial
peptides
(AMPs)
innate
immunity
are
among
most
promising
ones,
mainly
due
to
their
different
mechanisms
action
against
viruses
additional
biological
properties.
In
this
review,
main
physicochemical
characteristics
AMPs
described,
particular
interest
toward
derived
from
amphibian
skin.
Living
in
aquatic
terrestrial
environments,
amphibians
one
richest
sources
primary
secondary
structures.
Besides
describing
activities
these
underlying
mechanism,
review
aims
at
emphasizing
high
potential
small
development
agents
that
likely
reduce
selection
resistant
strains.
Antibiotics,
Год журнала:
2024,
Номер
13(11), С. 1076 - 1076
Опубликована: Ноя. 12, 2024
Antimicrobial
peptides
(AMPs)
from
amphibians
represent
a
promising
source
of
novel
antibacterial
agents
due
to
their
potent
and
broad-spectrum
antimicrobial
activity,
which
positions
them
as
valid
alternatives
conventional
antibiotics.
This
review
provides
comprehensive
analysis
the
mechanisms
through
amphibian-derived
AMPs
exert
effects
against
bacterial
pathogens.
We
focus
on
identification
protein
targets
implicated
in
action
these
biological
processes
altered
by
effect
AMPs.
By
examining
recent
advances
countering
multidrug-resistant
bacteria
multi-omics
approaches,
we
elucidate
how
interact
with
membranes,
enter
cells,
target
specific
protein.
discuss
implications
interactions
developing
targeted
therapies
overcoming
antibiotic
resistance
(ABR).
aims
integrate
current
knowledge
AMPs’
mechanisms,
identify
gaps
our
understanding,
propose
future
directions
for
research
harness
amphibian
clinical
applications.
Journal of Fungi,
Год журнала:
2025,
Номер
11(4), С. 311 - 311
Опубликована: Апрель 15, 2025
The
chytrid
fungus,
Batrachochytrium
dendrobatidis
(Bd),
infects
amphibian
skin,
causing
chytridiomycosis,
which
is
a
contributing
cause
of
worldwide
declines
and
extinctions
amphibians.
Relatively
little
known
about
the
roles
skin-resident
immune
cells,
such
as
macrophages,
in
these
antifungal
defenses.
Across
vertebrates,
macrophage
differentiation
controlled
through
activation
colony-stimulating
factor-1
(CSF1)
receptor
by
CSF1
interleukin-34
(IL34)
cytokines.
While
precise
respective
cytokines
development
remain
to
be
fully
explored,
our
ongoing
studies
indicate
that
frog
(Xenopus
laevis)
macrophages
differentiated
recombinant
forms
IL34
are
functionally
distinct.
Accordingly,
we
explored
X.
laevis
CSF1-
IL34-macrophages
anti-Bd
Enriching
cutaneous
IL34-macrophages,
but
not
CSF1-macrophages,
resulted
significant
protection.
In
vitro
analysis
macrophage-Bd
interactions
indicated
both
subsets
phagocytosed
Bd.
However,
cocultured
with
Bd
exhibited
greater
pro-inflammatory
gene
expression,
whereas
CSF1-macrophages
showed
immunosuppressive
expression
profiles.
Concurrently,
Bd-cocultured
possessed
elevated
genes
associated
evasion.
This
work
marks
step
forward
understanding
Abstract
Phylosymbiosis
is
an
association
between
host-associated
microbiome
composition
and
host
phylogeny.
This
pattern
can
arise
via
the
evolution
of
traits,
habitat
preferences,
diets,
co-diversification
hosts
microbes.
Understanding
drivers
phylosymbiosis
vital
for
modelling
disease-microbiome
interactions
manipulating
microbiomes
in
multi-host
systems.
study
quantifies
Appalachian
salamander
skin
context
infection
by
fungal
pathogen
Batrachochytrium
dendrobatidis
(Bd),
while
accounting
environmental
exposure.
We
sampled
ten
species
representing
>150M
years
divergence,
assessed
their
Bd
status,
analysed
microbiomes.
Our
results
reveal
a
significant
signal
phylosymbiosis,
whereas
local
pool
microbes,
climate,
geography,
load
had
smaller
impact.
Host-microbe
co-speciation
was
not
evident,
indicating
that
effect
stems
from
traits
influencing
assembly.
correlated
with
phylogeny
abundance
Bd-inhibitory
bacterial
strains,
suggesting
long-term
evolutionary
dynamics
affect
present-day
distribution
pathogen,
along
habitat-linked
exposure
risk.
Five
strains
showed
unusual
generalism:
occurring
most
habitats.
These
generalist
may
enhance
likelihood
probiotic
manipulations
colonising
persisting
on
hosts.
underscore
substantial
influence
host-microbiome
eco-evolutionary
health
disease
outcomes.
