Dact1 induces Dishevelled oligomerization to facilitate binding partner switch and signalosome formation during convergent extension DOI Creative Commons
Allyson Angermeier, Deli Yu, Yali Huang

и другие.

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Март 11, 2025

Convergent extension (CE) is a universal morphogenetic engine that promotes polarized tissue extension. In vertebrates, CE regulated by non-canonical Wnt ligands signaling through "core" proteins of the planar cell polarity (PCP) pathway, including cytoplasmic protein Dishevelled (Dvl), receptor Frizzled (Fz) and tetraspan Van gogh-like (Vangl). PCP was discovered in Drosophila to coordinate plane static epithelium, but does not regulate flies. Existing evidence suggests adopting for might be vertebrate-specific adaptation with incorporation new regulators. Herein we use Xenopus investigate Dact1, chordate-specific protein. Dact1 induces Dvl form oligomers dissociate from Vangl, stay attached Fz as signalosome-like clusters co-aggregate into patches upon induction. Functionally, antagonizes synergizes wild-type its oligomerization-defective mutants. We propose that, promoting oligomerization, couples binding partner switch cluster formation initiate during vertebrate CE. The action morphogenesis remains unclear. Here, authors find oligomerization couple Vangl cluster.

Язык: Английский

The functional roles of the circRNA/Wnt axis in cancer DOI Creative Commons
Chen Xue,

Ganglei Li,

Qiuxian Zheng

и другие.

Molecular Cancer, Год журнала: 2022, Номер 21(1)

Опубликована: Май 5, 2022

Abstract CircRNAs, covalently closed noncoding RNAs, are widely expressed in a wide range of species ranging from viruses to plants mammals. CircRNAs were enriched the Wnt pathway. Aberrant pathway activation is involved development various types cancers. Accumulating evidence indicates that circRNA/Wnt axis modulates expression cancer-associated genes and then regulates cancer progression. pathway-related circRNA obviously associated with many clinical characteristics. could regulate cell biological functions by interacting Moreover, circRNAs promising potential biomarkers for diagnosis, prognosis evaluation, treatment. In our review, we summarized recent research progress on role application tumorigenesis

Язык: Английский

Процитировано

97
The wing imaginal disc DOI Creative Commons
Bipin Kumar Tripathi, Kenneth D. Irvine

Genetics, Год журнала: 2022, Номер 220(4)

Опубликована: Март 4, 2022

Abstract The Drosophila wing imaginal disc is a tissue of undifferentiated cells that are precursors the and most notum adult fly. first forms during embryogenesis from cluster ∼30 located in second thoracic segment, which invaginate to form sac-like structure. They undergo extensive proliferation larval stages mature ∼35,000 cells. During this time, distinct cell fates assigned different regions, develops complex morphology. Finally, pupal undergoes morphogenetic processes then differentiates notum. While bulk comprises epithelial cells, it also includes neurons glia, associated with tracheal muscle precursor relative simplicity accessibility disc, combined wealth genetic tools available Drosophila, have make premier system for identifying genes deciphering systems play crucial roles animal development. Studies discs made key contributions many areas biology, including patterning, signal transduction, growth control, regeneration, planar polarity, morphogenesis, mechanics.

Язык: Английский

Процитировано

77
The Emerging Mechanisms of Wnt Secretion and Signaling in Development DOI Creative Commons

Shefali Mehta,

Swapnil Hingole, Varun Chaudhary

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9

Опубликована: Авг. 16, 2021

Wnts are highly-conserved lipid-modified secreted proteins that activate multiple signaling pathways. These pathways regulate crucial processes during various stages of development and maintain tissue homeostasis in adults. One the most fascinating aspects Wnt protein is despite being hydrophobic, they known to travel several cell distances extracellular space. Research on past four decades has identified factors uncovered mechanisms regulating their expression, secretion, mode travel. More recently, analyses importance gradients growth patterning developing tissues have recognized complex interplay help maintaining homeostasis. This review aims present an overview evidence for modes secretion discuss providing precision robustness tissues.

Язык: Английский

Процитировано

66
Regulation of Wnt/PCP signaling through p97/VCP-KBTBD7–mediated Vangl ubiquitination and endoplasmic reticulum–associated degradation DOI Creative Commons
Di Feng, Jin Wang, Wei Yang

и другие.

Science Advances, Год журнала: 2021, Номер 7(20)

Опубликована: Май 14, 2021

The abundance of core Wnt/PCP protein Vangl is regulated by the endoplasmic reticulum–associated degradation (ERAD) pathway.

Язык: Английский

Процитировано

55
No Evidence that Wnt Ligands Are Required for Planar Cell Polarity in Drosophila DOI Creative Commons
Ben Ewen‐Campen, Typhaine Comyn,

Eric Vogt

и другие.

Cell Reports, Год журнала: 2020, Номер 32(10), С. 108121 - 108121

Опубликована: Сен. 1, 2020

The frizzled (fz) and dishevelled (dsh) genes are highly conserved members of both the planar cell polarity (PCP) pathway Wnt signaling pathway. Given these dual functions, several studies have examined whether ligands provide a tissue-scale orientation cue for PCP establishment during development, reached differing conclusions. Here, we re-examine this issue in Drosophila melanogaster wing notum using split-Gal4 co-expression analysis, multiplex somatic CRISPR, double RNAi experiments. Pairwise loss-of-function experiments targeting wg together with other genes, via CRISPR or RNAi, do not produce defects notum. In addition, against evi (aka wntless), which is required secretion ligands, did detectable phenotypes. Altogether, our results support hypothesis that contribute to

Язык: Английский

Процитировано

52
How do the Fat–Dachsous and core planar polarity pathways act together and independently to coordinate polarized cell behaviours? DOI Creative Commons
Helen Strutt, David Strutt

Open Biology, Год журнала: 2021, Номер 11(2)

Опубликована: Фев. 1, 2021

Planar polarity describes the coordinated polarization of cells within plane a tissue. This is controlled by two main pathways in Drosophila : Frizzled-dependent core planar pathway and Fat–Dachsous pathway. Components both these become asymmetrically localized response to long-range upstream cues, form intercellular complexes that link between neighbouring cells. review examines if when are coupled, focusing on wing, eye abdomen. There strong evidence molecularly coupled tissues express specific isoform protein Prickle, namely Spiny-legs. However, other contexts, linkages indirect. We discuss how act together independently mediate diverse range effects cell structures behaviours.

Язык: Английский

Процитировано

51
Planar cell polarity coordination in a cnidarian embryo provides clues to animal body axis evolution DOI Open Access

Julie Uveira,

Antoine Donati,

Marvin Léria

и другие.

Опубликована: Янв. 28, 2025

Body axis specification is a crucial event in animal embryogenesis and was an essential evolutionary innovation for founding the kingdom. It involves two distinct components that coordinate to establish spatial organisation of embryo: initiation cascades regionalised gene expression orientation morphogenetic processes such as body elongation. Intense interest first component has revealed Wnt/β-catenin signalling ancestrally responsible initiating regional expression, but origin oriented morphogenesis received little attention. Here, by addressing cell morphological basis development embryos cnidarian Clytia hemisphaerica, we have uncovered simple likely ancestral coordination mechanism between directed morphogenesis. We show ligand Wnt3, known initiate oral via localised pathway activation, also key β-catenin-independent role globally orienting planar polarity (PCP) direct along oral-aboral axis. This PCP occurs steps: local Wnt3 global propagation conserved core protein interactions From these findings propose novel scenarios PCP-driven symmetry-breaking underlying emergence plan.

Язык: Английский

Процитировано

1
Tissue shear as a cue for aligning planar polarity in the developing Drosophila wing DOI Creative Commons
Su Tan, David Strutt

Nature Communications, Год журнала: 2025, Номер 16(1)

Опубликована: Фев. 7, 2025

Abstract Planar polarity establishment in epithelia requires interpretation of directional tissue-level information at cellular and molecular levels. Mechanical forces exerted during tissue morphogenesis are emerging as crucial cues, yet the mechanisms by which they regulate planar poorly understood. Using Drosophila pupal wing, we confirm that stress promotes proximal-distal (PD) alignment. Moreover, high anisotropy can reduce rate accumulation lower stability on cell junctions core protein Frizzled (Fz). Notably, under anisotropy, see an increased gradient flow, characterised differential velocities across adjacent rows. This turnover cell-cell contacts parallel to flow direction, possibly via dissociation transmembrane complexes shear forces. We propose gradients play a critical role establishing maintaining PD-oriented alignment developing wing.

Язык: Английский

Процитировано

1
Vangl as a Master Scaffold for Wnt/Planar Cell Polarity Signaling in Development and Disease DOI Creative Commons

Courtney A. Dreyer,

Kacey VanderVorst,

Kermit L. Carraway

и другие.

Frontiers in Cell and Developmental Biology, Год журнала: 2022, Номер 10

Опубликована: Май 11, 2022

The establishment of polarity within tissues and dynamic cellular morphogenetic events are features common to both developing adult tissues, breakdown these programs is associated with diverse human diseases. Wnt/Planar cell (Wnt/PCP) signaling, a branch non-canonical Wnt critical the maintenance in epithelial as well motility proper embryonic development. In Wnt/PCP-mediated planar relies upon asymmetric distribution core proteins establish polarity, but requirement for this remains unclear. However, polarized migratory cells, Wnt/PCP-specific transmembrane protein Vangl required appears serve scaffold which pathway components positive negative regulators Wnt/PCP signaling assemble. current literature suggests that multiple interaction domains allow binding partners context- tissue-specific complexes. review we discuss role master tissue address how dysregulated disease.

Язык: Английский

Процитировано

26
Spatial and temporal regulation of Wnt signaling pathway members in the development of butterfly wing patterns DOI Creative Commons
Tirtha Das Banerjee, Suriya Narayanan Murugesan, Heidi Connahs

и другие.

Science Advances, Год журнала: 2023, Номер 9(30)

Опубликована: Июль 26, 2023

Wnt signaling members are involved in the differentiation of cells associated with eyespot and band color patterns on wings butterflies, but identity spatio-temporal regulation specific pathway remains unclear. Here, we explore localization function Armadillo/β-catenin dependent (canonical) independent (noncanonical) development

Язык: Английский

Процитировано

17