Developmental Biology, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Nature, Год журнала: 2022, Номер 605(7909), С. 325 - 331
Опубликована: Апрель 13, 2022
Язык: Английский
Процитировано
248
International Journal of Molecular Sciences, Год журнала: 2022, Номер 23(3), С. 1464 - 1464
Опубликована: Янв. 27, 2022
Damage to organs by trauma, infection, diseases, congenital defects, aging, and other injuries causes organ malfunction is life-threatening under serious conditions. Some of the lower order vertebrates such as zebrafish, salamanders, chicks possess superior regenerative capacity over mammals. The extracellular signal-regulated kinases 1 2 (ERK1/2), key members mitogen-activated protein kinase (MAPK) family, are serine/threonine that phylogenetically conserved among vertebrate taxa. MAPK/ERK signaling an irreplaceable player participating in diverse biological activities through phosphorylating a broad variety substrates cytoplasm well inside nucleus. Current evidence supports central role pathway during regeneration processes. rapidly excited response injury stimuli coordinates essential pro-regenerative cellular events including cell survival, fate turnover, migration, proliferation, growth, transcriptional translational activities. In this literature review, we recapitulated multifaceted regulations, its dynamic spatio-temporal activities, profound roles multiple regeneration, appendages, heart, liver, eye, peripheral/central nervous system, illuminating possibility critical mechanism underlying vastly differential capacities species, potential applications tissue engineering medicine.
Язык: Английский
Процитировано
79
Science, Год журнала: 2022, Номер 377(6610)
Опубликована: Сен. 1, 2022
Salamanders are tetrapod models to study brain organization and regeneration; however, the identity evolutionary conservation of cell types largely unknown. We delineated populations in axolotl telencephalon during homeostasis regeneration using single-cell genomic profiling. identified glutamatergic neurons with similarities amniote hippocampus, dorsal lateral cortex, conserved γ-aminobutyric acid-releasing (GABAergic) neuron classes. inferred transcriptional dynamics gene regulatory relationships postembryonic, region-specific neurogenesis unraveled differentiation signatures. After injury, ependymoglia activate an injury-specific state before reestablishing lost axonal connections. Together, our analyses yield insights into organization, evolution, a nervous system.
Язык: Английский
Процитировано
76
Science, Год журнала: 2023, Номер 379(6634), С. 840 - 847
Опубликована: Фев. 24, 2023
The annual regrowth of deer antlers provides a valuable model for studying organ regeneration in mammals. We describe single-cell atlas antler regrowth. earliest-stage initiators were mesenchymal cells that express the paired related homeobox 1 gene ( PRRX1 + cells). also identified population “antler blastema progenitor cells” (ABPCs) developed from and directed process. Cross-species comparisons ABPCs several mammalian blastema. In vivo vitro displayed strong self-renewal ability could generate osteochondral lineage cells. Last, we observed spatially well-structured pattern cellular expression growth center during peak stage, revealing mechanisms involved rapid elongation.
Язык: Английский
Процитировано
59
Cell stem cell, Год журнала: 2023, Номер 30(9), С. 1130 - 1147
Опубликована: Авг. 24, 2023
Язык: Английский
Процитировано
42
Nature Reviews Molecular Cell Biology, Год журнала: 2024, Номер 25(9), С. 720 - 739
Опубликована: Апрель 29, 2024
Язык: Английский
Процитировано
24
Frontiers in Cell and Developmental Biology, Год журнала: 2021, Номер 9
Опубликована: Окт. 1, 2021
Zebrafish have been found to be a premier model organism in biological and regeneration research. However, the comprehensive cell compositions molecular dynamics during tissue zebrafish remain poorly understood. Here, we utilized Microwell-seq analyze more than 250,000 single cells covering major types constructed systematic landscape. We revealed single-cell for 18 both embryo adult stages. Single-cell mapping of caudal fin unique characteristic blastema population key genetic regulation involved repair. Overall, our datasets demonstrate utility landscape resources various fields
Язык: Английский
Процитировано
75
Nature Ecology & Evolution, Год журнала: 2023, Номер 7(12), С. 2108 - 2124
Опубликована: Окт. 19, 2023
Regenerative abilities vary dramatically across animals. Even amongst planarian flatworms, well-known for complete regeneration from tiny body fragments, some species have restricted while others are almost entirely incompetent. Here, we assemble a diverse live collection of 40 to probe the evolution head in group. Combining quantification species-specific head-regeneration with comprehensive transcriptome-based phylogeny reconstruction, show multiple independent transitions between robust whole-body and freshwater species. RNA-mediated genetic interference inhibition canonical Wnt signalling interference-sensitive bypassed all defects, suggesting that pathway is linked emergence defects. Our finding has roles reproductive system model Schmidtea mediterranea raises possibility trade-off egg-laying, asexual reproduction by fission/regeneration drives regenerative trait evolution. Although quantitative comparisons levels, yolk content strategy our remained inconclusive, they revealed divergent planarians. Altogether, study establishes planarians as taxon comparative research presents framework mechanistic abilities.
Язык: Английский
Процитировано
29
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(4), С. 2172 - 2172
Опубликована: Фев. 11, 2024
Fibroblasts are stromal cells ubiquitously distributed in the body of nearly every organ tissue. These were previously considered to be “passive cells”, solely responsible for ensuring turnover extracellular matrix (ECM). However, their versatility, including ability switch phenotypes response tissue injury and dynamic activity maintenance specific homeostasis integrity have been recently revealed by innovation technological tools such as genetically modified mouse models single cell analysis. highly plastic heterogeneous equipped with multifaceted functions regulation angiogenesis, inflammation well innate stemness characteristics, play a central role delicately regulated process wound healing. Fibroblast dysregulation underlies many chronic conditions, cardiovascular diseases, cancer, inflammatory diabetes mellitus (DM), which represent current major causes morbidity mortality worldwide. Diabetic foot ulcer (DFU), one most severe complications DM affects 40 60 million people. Chronic non-healing DFU wounds expose patients substantial sequelae infections, gangrene, amputation, death. A complete understanding pathophysiology targeting pathways involved fibroblasts required development innovative new therapeutic treatments, critically needed these patients.
Язык: Английский
Процитировано
14
Developmental Cell, Год журнала: 2024, Номер 59(16), С. 2239 - 2253.e9
Опубликована: Май 23, 2024
The salamander limb correctly regenerates missing segments because connective tissue cells have segment-specific identities, termed "positional information". How positional information is molecularly encoded at the chromatin level has been unknown. Here, we performed genome-wide profiling in mature and regenerating axolotl cells. We find levels of histone H3K27me3 as major mark, especially homeoprotein gene loci but not their upstream regulators, constituting an intrinsic segment code. During regeneration, regeneration-specific regulatory elements became active prior to re-appearance developmental elements. In hand, permissive state HoxA13 engages with regeneration program bypassing upper program. Comparison those found other regenerative animals identified a core shared set transcription factors, supporting ancient, conserved
Язык: Английский
Процитировано
12