Cell Reports Methods, Год журнала: 2024, Номер 4(11), С. 100897 - 100897
Опубликована: Ноя. 1, 2024
Язык: Английский
Cell, Год журнала: 2024, Номер 187(22), С. 6152 - 6164.e18
Опубликована: Сен. 25, 2024
Язык: Английский
Процитировано
49
Nature Chemical Biology, Год журнала: 2025, Номер unknown
Опубликована: Янв. 3, 2025
Язык: Английский
Процитировано
4
Cell Insight, Год журнала: 2024, Номер 3(4), С. 100180 - 100180
Опубликована: Июнь 27, 2024
Cell fate determination is an intricate process which orchestrated by multiple regulatory layers including signal pathways, transcriptional factors, epigenetic modifications, and metabolic rewiring. Among the sophisticated modulations, repressive mark H3K27me3, deposited PRC2 (polycomb complex 2) removed demethylase KDM6, plays a pivotal role in mediating cellular identity transition through its dynamic precise alterations. Herein, we overview discuss how H3K27me3 modifiers regulate pluripotency maintenance early lineage differentiation. We primarily highlight following four aspects: 1) two subcomplexes PRC2.1 PRC2.2 distribution of genomic H3K27 methylation; as critical regulator exit; 3) emerging eraser KDM6 differentiation; 4) newly identified additional factors influencing H3K27me3. present comprehensive insight into molecular principles regulation well this participates pluripotent stem cell-centered cell determination.
Язык: Английский
Процитировано
6
Acta Materia Medica, Год журнала: 2025, Номер 4(1)
Опубликована: Янв. 1, 2025
Complex biological mechanisms and unidentified therapeutic targets for amyotrophic lateral sclerosis (ALS) significantly hinder the development of effective treatments. Given these challenges, reliable disease models that accurately replicate ALS phenotypes with relevant underpinnings are essential advancing precision medicine in ALS. Patient-derived induced pluripotent stem cell (iPSC) organoids have emerged as an innovative tool modeling drug evaluation. Growing evidence highlights advantages replicating supporting development. However, challenges remain utilizing testing other neurodegenerative diseases. In this review we summarize current progress model development, encompassing both vitro vivo non-human models, well iPSC-derived human models. Furthermore, within context screening, discuss critical considerations applying to evaluate disease-associated reflect disease-related symptoms.
Язык: Английский
Процитировано
0
BioDrugs, Год журнала: 2025, Номер unknown
Опубликована: Фев. 7, 2025
Язык: Английский
Процитировано
0
ACTA HISTOCHEMICA ET CYTOCHEMICA, Год журнала: 2025, Номер 58(1), С. 19 - 30
Опубликована: Фев. 26, 2025
Spinal cord injury (SCI) is incurable and often leads to permanent motor dysfunction, paralysis, sensory impairment. We previously developed a method directly reprogram human fibroblasts into neuron-like cells using only chemical compounds. In rat model of SCI, we transplanted chemically reprogrammed cells, termed immature chemical-induced (CiN) derived the with slight modifications found that CiN exhibited therapeutic efficacy in SCI. As primate models more closely mimic humans than models, experiments are required accurately assess safety before their use humans. Therefore, this study, aimed determine cell transplantation marmoset SCI model. Immature were subacute on Day 9 after contusion injury, was assessed. Motor recovery assessed based spontaneous activity original open-field rating scale over six weeks, which spinal at site subjected histopathological MRI analyses. Animals significantly enhanced compared control animals, consistent improved nerve or preservation. Our findings suggest can effectively treat primates.
Язык: Английский
Процитировано
0
Journal of Translational Medicine, Год журнала: 2025, Номер 23(1)
Опубликована: Фев. 27, 2025
Direct reprogramming has garnered considerable attention due to its capacity directly convert differentiated cells into desired cells. Fibroblasts are frequently employed in studies their abundance and accessibility. However, they also the key drivers progression of fibrosis, a pathological condition characterized by excessive extracellular matrix deposition tissue scarring. Furthermore, initial stage typically involves deactivating fibrotic pathways. Hence, direct offers valuable method regenerate target for repair while simultaneously reducing tendencies. Understanding link between fibrosis could help develop effective strategies treat damaged with potential risk fibrosis. This review summarizes advances reveals anti-fibrosis effects various organs such as heart, liver, skin. we dissect mechanisms influenced molecules including TGF-β signaling, mechanical inflammation epigenetic modifiers, metabolic regulators. Innovative methods fibroblast like small molecules, CRISPRa, modified mRNA, challenges cellular heterogeneity senescence faced vivo reprogramming, discussed.
Язык: Английский
Процитировано
0
Diabetes & Metabolism, Год журнала: 2025, Номер unknown, С. 101635 - 101635
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0
Biomedicines, Год журнала: 2025, Номер 13(4), С. 765 - 765
Опубликована: Март 21, 2025
The field of induced pluripotent stem cells (iPSCs) continues to evolve, offering unprecedented potential for regenerative medicine, disease modeling, and therapeutic applications [...]
Язык: Английский
Процитировано
0
Trends in Biochemical Sciences, Год журнала: 2025, Номер unknown
Опубликована: Март 1, 2025
Язык: Английский
Процитировано
0