bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Ноя. 1, 2024
Abstract
Mammalian
cancer
cell
lines
are
essential
model
systems
in
biomedical
research.
We
conducted
multi-level
proteomics
analyses
on
54
widely
used
derived
from
various
tissue-of-origins
using
two
prominent
technologies:
mass
spectrometry
(MS)
and
reverse-phase
protein
array
(RPPA).
Our
analysis
identified
10,088
proteins,
33,609
phosphorylation
sites
across
7,289
phosphoproteins,
56,350
site-specific
glycans
16,296
glycosylation
5,966
glycoproteins,
along
with
305
drug-relevant
phosphoprotein
targets.
results
reveal
both
consistent
distinct
patterns
expression
modification
between
MS
RPPA,
underscoring
their
complementary
strengths
as
discovery
tools.
Additionally,
we
features
that
distinguish
tissue
origins
different
line
lineages.
This
dataset
supports
system
selection
for
drug
target-related
studies
vitro
provides
valuable
insights
into
key
signaling
pathways.
Overall,
this
comprehensive
resource
enables
new
opportunities
exploration
biology
offers
significant
value
to
research
communities
focused
biomarker
profiling,
target
discovery,
understanding
mechanisms
diverse
types.
Frontiers in Pharmacology,
Год журнала:
2024,
Номер
15
Опубликована: Май 2, 2024
The
therapeutic
effect
of
chemotherapy
and
targeted
therapy
are
known
to
be
limited
by
drug
resistance.
Substantial
evidence
has
shown
that
ATP-binding
cassette
(ABC)
transporters
P-gp
BCRP
significant
contributors
multidrug
resistance
(MDR)
in
cancer
cells.
In
this
study,
we
demonstrated
a
clinical-staged
ATR
inhibitor
ceralasertib
is
susceptible
BCRP-mediated
MDR.
resistant
cells
were
less
sensitive
compared
the
parental
Moreover,
can
reversed
inhibiting
efflux
activity
BCRP.
Interestingly,
was
able
downregulate
level
but
not
BCRP,
suggesting
potential
regulation
between
signaling
expression.
Furthermore,
computational
docking
analysis
predicted
high
affinities
drug-binding
sites
summary,
overexpression
sufficient
confer
ceralasertib,
underscoring
their
role
as
biomarkers
for
efficacy.
World Journal of Gastrointestinal Oncology,
Год журнала:
2025,
Номер
17(5)
Опубликована: Май 15, 2025
BACKGROUND
Colorectal
cancer
(CRC)
is
the
second
most
prevalent
cause
of
cancer-related
mortality
and
increasing
in
younger
individuals.
Chemotherapy,
a
crucial
adjuvant
systemic
therapy
for
CRC
management,
often
leads
to
resistance
through
poorly
characterized
underlying
molecular
mechanisms.
The
long
noncoding
RNA
SNHG5
highly
expressed
promotes
tumor
proliferation
invasion,
prompting
us
hypothesize
that
may
play
role
chemotherapeutic
agent
5-fluorouracil
(5-Fu)
CRC.
AIM
To
identify
function
mechanism
5-Fu
METHODS
Quantitative
real-time
polymerase
chain
reaction
was
performed
examine
expression
tissues
from
22
5-Fu-sensitive
patients
14
5-Fu-resistant
cells
cells.
Cell
viability
apoptosis
were
assessed
-overexpressing
-knockdown
further
analyzed
using
xenograft
mouse
model.
interactions
with
microRNAs
predicted
by
bioinformatics
analysis.
Luciferase
reporter
immunoprecipitation
assays
verify
binding
between
miR-26b.
Rescue
experiments
validate
functional
interaction
miR-26b/p-glycoprotein
(Pgp)
axis.
RESULTS
upregulated
In
vitro
demonstrated
overexpression
significantly
reduced
cell
enhanced
viability,
whereas
knockdown
increased
decreased
upon
treatment.
model,
we
confirmed
led
reduction
sensitivity
vivo
.
Mechanistically,
acted
as
sponge
validated
conferred
regulating
miR-26b/Pgp
CONCLUSION
/miR-26b/Pgp
regulates
chemosensitivity,
providing
potential
therapeutic
targets
treatment
Journal of Investigative Surgery,
Год журнала:
2024,
Номер
37(1)
Опубликована: Ноя. 28, 2024
Background
The
high
invasion
and
heterogeneity
of
head
neck
squamous
cell
carcinoma
(HNSCC)
commonly
leads
to
poor
clinical
outcomes.
Identification
reliable
biomarkers
for
HNSCC
is
imperative.
iScience,
Год журнала:
2024,
Номер
27(11), С. 111072 - 111072
Опубликована: Окт. 5, 2024
Chemotherapy
resistance
is
still
a
great
challenge
for
clinical
treatment
of
lung
cancer.
Here,
we
found
that
doxorubicin
(DOX)
induced
an
increase
labile
Zn
