A Coumarin‐Based Reversible Fluorescent Probe for Quantitative Detection and Monitoring of Cellular Glutathione

ChemistrySelect, Год журнала: 2025, Номер 10(12)

Опубликована: Март 1, 2025

Abstract An “on‐off‐on” reversible fluorescent probe for response to GSH and H 2 O determination was designed synthesized. Considering the important role of in biological system commonly applied recognition groups, C═C bond coumarin dye successfully used as site. This showed fast (within 5 s) high sensitivity (12.6 nM) process. It could be not only quantitative GSH, but also fluorescence imaging cells. In addition, , which repeated at least 3 times. The experimental results proved that modified from recognized with significant results.

Язык: Английский

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A simple BODIPY-based fluorescent probe for sequential recognition of Cu2+ and GSH and its application on test strips and bioimaging in living cells

Journal of Molecular Structure, Год журнала: 2023, Номер 1294, С. 136393 - 136393

Опубликована: Авг. 10, 2023

Язык: Английский

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Dual-channel luminescent Ir(III) complex for detection of GSH and Hcy/Cys in cells

Biosensors and Bioelectronics, Год журнала: 2023, Номер 246, С. 115901 - 115901

Опубликована: Дек. 1, 2023

Язык: Английский

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Modification of an AIE Fluorescent Probe for Monitoring the Polarity of Lipid Droplets Based on a series of Synthesized Aryl Naphthalizes

Chemistry - A European Journal, Год журнала: 2024, Номер 30(58)

Опубликована: Авг. 6, 2024

Lipid droplets (LDs) are subcellular organelles that dynamic and play a central role in energy homeostasis lipid metabolism. They also contribute to the transport maturation of cellular proteins closely associated with several diseases. The important microenvironment maintaining homeostasis. Changes cell polarity, particularly organelles, have been found be strongly linked inflammation, Alzheimer's disease, cancer, other illnesses. It is essential check polarity LDs. A series arylated naphthalimide derivatives were synthesized using Suzuki reaction. Modification aryl naphthalimides oligomeric PEG based on intramolecular charge transfer (ICT) mechanism. fluorescent probes designed target LDs detect their polarity. Nap-TPA-PEG

Язык: Английский

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Recent process in organic small molecular fluorescent probes for tracking markers of tumor redox balance

TrAC Trends in Analytical Chemistry, Год журнала: 2023, Номер 170, С. 117461 - 117461

Опубликована: Ноя. 23, 2023

Exploring the redox balance in tumor region is of great significance for clarifying pathogenesis tumors and finding methods treatment tumors. Tumors can maintain specific by expression some types reactive oxygen species (for instance O2•−, H2O2, ONOO−, etc), reducing substances (GSH, H2Sn, Cys). These thus be used as markers to identify changes cancer cells or microenvironments. However, their high reactivity makes them difficult rapidly detected in-situ biosystems. To solve this problem, various organic small molecular fluorescent probes (OSMFPs) have been developed relying on fluorescence imaging technology. In review, we systematically introduced recent progress about OSMFPs that could detect proposed outlooks based our opinions. This review provide important reference further development OSMFPs-based detection balance.

Язык: Английский

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Visualizing ClO− fluxes during homocysteine stress based on a nanoprobe

Dyes and Pigments, Год журнала: 2023, Номер 213, С. 111163 - 111163

Опубликована: Фев. 13, 2023

Язык: Английский

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Fluorescent Coumarin-based Probe for Detection of Biological Thiols

Current Organic Chemistry, Год журнала: 2023, Номер 27(15), С. 1329 - 1335

Опубликована: Авг. 1, 2023

Abstract: Nature-derived thiols such as cysteine, homocysteine, and reduced glutathione play diverse important roles in both plant animal cells. In plants, they maintain redox homeostasis affect photosynthesis signal transduction processes. animals, changes the levels of biothiols have been linked to cardiovascular disease, Alzheimer's neuropsychiatric disorders, or stroke. The detection biological samples is, therefore, very important. For this purpose, rapid, sensitive, non-invasive methods for their sensing living cells are highly desirable. : This perspective reviews past five years advances coumarin fluorescent probes biothiol detection. design probes, mechanisms thiol detection, applications live cell imaging presented. classification based on backbone was made basis mode interactions with analyte.

Язык: Английский

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Water-Soluble Dual-Channel Fluorescent Probe for Sensitive Detection of Biothiols In Vitro and In Vivo

ACS Applied Bio Materials, Год журнала: 2023, Номер 6(12), С. 5828 - 5835

Опубликована: Дек. 6, 2023

Benefiting from high spatiotemporal resolution, deep tissue penetration, and excellent sensitivity, fluorescence imaging technology has been widely applied in cancer diagnosis treatment. In recent years, a large number of fluorescent probes for monitoring the levels endogenous biothiols have reported, which significant implications However, most still suffer poor biological compatibility easy attachment by environment. This work presents development water-soluble dual-channel probe, named MAL-NBD, sensitively detecting biothiols. Nonfluorescent MAL-NBD is transformed into groups MAL NBD-SR/NR through nucleophilic substitution biologically active thiols, producing signals precise detection thiols. Taking advantage biocompatibility low biotoxicity, successfully used HeLa cells zebrafish larvae, promoting its potential application thiols involved physiological pathological processes.

Язык: Английский

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Bioimaging and Sensing Thiols In Vivo and in Tumor Tissues Based on a Near-Infrared Fluorescent Probe with Large Stokes Shift

Molecules, Год журнала: 2023, Номер 28(15), С. 5702 - 5702

Опубликована: Июль 27, 2023

The well-known small-molecule biothiols have been used to maintain the normal metabolism of peroxy radicals, forming protein structures, resisting cell apoptosis, regulating metabolism, and protecting homeostasis cells in organism. A large amount research has found that abnormal levels above can cause some adverse diseases, such as changes hair pigmentation, a slower growth rate, delayed response, excessive sleep skin diseases. In order further investigate exact intracellular molecular mechanism biothiols, it is imperative explore effective strategies for real-time biothiol detection living systems. this work, new near-infrared (NIR) emission fluorescence probe (probe 1) sensitive selective was devised by combining dicyanoisophorone derivatives with dinitrobenzenesulfonyl (DNBS) group. As expected, 1 could specifically detect (Cys, Hcy GSH) through group form dye 2, which works signaling molecule sensing real samples. Surprisingly, showed superior characteristics low-limit towards (36.0 nM Cys, 39.0 48.0 Stokes shift (134 nm). Additionally, function platform detecting confirmed confocal imaging MCF-7 zebrafish. More importantly, capability vivo evaluated overexpressed tumor tissue. It reasonable believe provide valuable method relationship between genesis tumor.

Язык: Английский

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A high-stable and fast-responsive lysosome-targeted probe for detection of endogenous and exogenous HClO in living cells and its application in zebrafish imaging

Journal of Molecular Structure, Год журнала: 2024, Номер unknown, С. 138804 - 138804

Опубликована: Июнь 1, 2024

Язык: Английский

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