Enhancing mosquito classification through self-supervised learning DOI Creative Commons

Ratana Charoenpanyakul,

Veerayuth Kittichai, Songpol Eiamsamang

и другие.

Scientific Reports, Год журнала: 2024, Номер 14(1)

Опубликована: Ноя. 7, 2024

Traditional mosquito identification methods, relied on microscopic observation and morphological characteristics, often require significant expertise experience, which can limit their effectiveness. This study introduces a self-supervised learning-based image classification model using the Bootstrap Your Own Latent (BYOL) algorithm, designed to enhance species efficiently. The BYOL algorithm offers key advantage by eliminating need for labeled data during pretraining, as it autonomously learns important features. During fine-tuning, requires only small fraction of achieve accurate results. Our approach demonstrates impressive performance, achieving over 96.77% accuracy in analysis, with minimized both false positives negatives. Additionally, model's overall accuracy, measured area under ROC curve, surpasses 99.55%, highlighting its robustness reliability. A notable finding is that fine-tuning just 10% produces results comparable full dataset. particularly valuable resource-limited settings limited access advanced equipment expertise. provides practical solution identification, overcoming challenges traditional such time-consuming process reliance specialized knowledge healthcare services. Overall, this supports personnel resource-constrained environments facilitating vector density analysis paving way future methodologies.

Язык: Английский

Zika virus: advancing a priority research agenda for preparedness and response DOI
Eve Lackritz, Lee Ching Ng, Ernesto T. A. Marques

и другие.

The Lancet Infectious Diseases, Год журнала: 2025, Номер unknown

Опубликована: Фев. 1, 2025

Язык: Английский

Процитировано

2

Global wastewater surveillance for pathogens with pandemic potential: opportunities and challenges DOI Creative Commons
Nicholas C. Grassly, Alex Shaw, Michael Owusu

и другие.

The Lancet Microbe, Год журнала: 2024, Номер unknown, С. 100939 - 100939

Опубликована: Авг. 1, 2024

SummaryWastewater surveillance holds great promise as a sensitive method to detect spillover of zoonotic infections and early pandemic emergence, thereby informing risk mitigation public health response. Known viruses with potential are shed in human stool or urine, both, the experiences SARS-CoV-2, monkeypox virus, Zika virus highlight feasibility community-based wastewater for that have different transmission routes. We reviewed shedding data prototype representing viral families concern estimate likely sensitivity compared clinical surveillance. examined how on detection, together genetic sequences animal faecal biomarkers, could be used identify adaptation. The opportunities challenges associated global prevention pandemics described this Personal View, focusing low-income middle-income countries, where emergence is highest. propose research agenda ensure an equitable sustainable solution these challenges.

Язык: Английский

Процитировано

13

Solid–liquid partitioning of dengue, West Nile, Zika, hepatitis A, influenza A, and SARS-CoV-2 viruses in wastewater from across the USA DOI Creative Commons
Laura Roldan-Hernandez, Camila Van Oost, Alexandria B. Boehm

и другие.

Environmental Science Water Research & Technology, Год журнала: 2024, Номер unknown

Опубликована: Янв. 1, 2024

Limited information is available on the fate of respiratory and arthropod-borne viruses in wastewater.

Язык: Английский

Процитировано

12

Usefulness of aircraft and airport wastewater for monitoring multiple pathogens including SARS-CoV-2 variants DOI Creative Commons
Martin Tay, Benjamin Lee, Muhammad Hafiz Ismail

и другие.

Journal of Travel Medicine, Год журнала: 2024, Номер 31(5)

Опубликована: Май 29, 2024

As global travel resumed in coronavirus disease 2019 (COVID-19) endemicity, the potential of aircraft wastewater monitoring to provide early warning trends for severe acute respiratory syndrome 2 (SARS-CoV-2) variants and other infectious diseases, particularly at international air hubs, was recognized. We therefore assessed compared feasibility testing from inbound airport terminals 18 pathogens including SARS-CoV-2 Singapore, a popular hub Asia.

Язык: Английский

Процитировано

4

Modelling the impact and cost-effectiveness of upcoming Zika virus vaccines on congenital Zika syndrome DOI Creative Commons
He Song, Shihui Jin,

Bing Yang

и другие.

Vaccine, Год журнала: 2025, Номер 48, С. 126728 - 126728

Опубликована: Янв. 16, 2025

Язык: Английский

Процитировано

0

A Perspective on Wastewater and Environmental Surveillance as a Public Health Tool for Low- and Middle-Income Countries DOI Creative Commons
Mohammad S. Khan, Christian Wurzbacher, Anna Uchaikina

и другие.

Microorganisms, Год журнала: 2025, Номер 13(2), С. 238 - 238

Опубликована: Янв. 22, 2025

Geographical variations in infectious diseases create differences public health priorities between high- and low-income countries. Low- middle-income countries (LMICs) face resource constraints that limit adherence to international monitoring standards for wastewater-based epidemiology (WBE). The development of low-cost WBE programs, such as those detect SARS-CoV-2, offers LMICs a promising tool pathogens local concern. In this work, we summarize important wastewater biomarkers their associated challenges, ranging from causing gastroenteritis putative markers plant linked food safety, well antimicrobial resistance. We raise awareness the great potential highlight critical markers, research needs, strategies necessary establish tailored surveillance programs.

Язык: Английский

Процитировано

0

Surveillance of Emerging Rodent-Borne Pathogens in Wastewater in Taiwan: A One Health Approach DOI Creative Commons
Kun‐Hsien Tsai, Tsai‐Ying Yen, Hsin-hsin Tung

и другие.

Tropical Medicine and Infectious Disease, Год журнала: 2024, Номер 9(11), С. 282 - 282

Опубликована: Ноя. 18, 2024

Leptospirosis and hantavirus syndrome are two major rodent-borne diseases in Taiwan.

Язык: Английский

Процитировано

1

Wastewater surveillance and Wolbachia-aedes method to combat Zika virus outbreaks in tropical countries DOI
Isaac KS Ng

Tropical Doctor, Год журнала: 2024, Номер 54(3), С. 295 - 295

Опубликована: Апрель 22, 2024

Язык: Английский

Процитировано

0

寨卡病毒E蛋白和NS1蛋白关键氨基酸突变可促进病毒在小鼠睾丸中的复制 DOI Creative Commons
Kai Li, Xiuli Yan,

Mengjiao He

и другие.

Chinese Science Bulletin (Chinese Version), Год журнала: 2024, Номер unknown

Опубликована: Апрель 1, 2024

寨卡病毒(Zika virus,ZIKV)是一种蚊媒黄病毒,也可通过性传播,同时还能感染睾丸组织并导致损伤。探究寨卡病毒在睾丸内持续复制过程中病毒基因的变化规律,发现潜在的睾丸适应性突变位点,可为寨卡病毒变异的风险评估提供重要参考。为此,本研究以寨卡病毒FSS13025株为研究对象,通过将其在A129小鼠睾丸中连续传代10轮后获得适应性传代毒株,将原始株(WT)、第5代(P5)和第10代(P10)病毒经腹腔接种A129小鼠后发现,睾丸中P5和P10的RNA载量较WT分别提高4.6倍和3.8倍;进一步通过深度测序分析P5和P10的基因组变异情况,结果显示二者的E蛋白和NS1蛋白分别出现H401Y和K146E突变。在此基础上,以寨卡病毒FSS13025株为基因骨架,利用反向遗传学技术构建拯救携带上述突变的重组病毒H401Y+K146E,并评价其在细胞和小鼠体内的复制能力,结果显示感染睾丸细胞15P-1和TM3后第48 h,细胞培养上清中的病毒RNA含量分别是WT的5.2倍和2.1倍;经腹腔途径感染A129小鼠后的第3和6天,睾丸中重组病毒RNA载量较WT分别提高97.5倍和58.4倍。上述结果表明,H401Y+K146E联合突变显著增强了寨卡病毒在睾丸细胞中的复制能力,同时明显提升了其对睾丸的嗜性和侵袭力,上述位点可作为寨卡病毒变异风险预警的监测靶点。

Процитировано

0

The Impact of Climate on Human Dengue Infections in the Caribbean DOI Creative Commons
Kirk Osmond Douglas,

Karl Payne,

Gilberto Sabino‐Santos

и другие.

Pathogens, Год журнала: 2024, Номер 13(9), С. 756 - 756

Опубликована: Сен. 3, 2024

Climate change is no longer a hypothetical problem in the Caribbean but new reality to which regional public health systems must adapt. One of its significant impacts increased transmission infectious diseases, such as dengue fever, endemic region, and presence

Язык: Английский

Процитировано

0