Curcumin-incorporated EGCG-based nano-antioxidants alleviate colon and kidney inflammation via antioxidant and anti-inflammatory therapy DOI Creative Commons
Qingqing Pan, Li Xie, Zhu Huang

и другие.

Regenerative Biomaterials, Год журнала: 2024, Номер 11

Опубликована: Янв. 1, 2024

Abstract Natural remedies are gaining attention as promising approaches to alleviating inflammation, yet their full potential is often limited by challenges such poor bioavailability and suboptimal therapeutic effects. To overcome these limitations, we have developed a novel nano-antioxidant (EK) based on epigallocatechin gallate (EGCG) aimed at enhancing the oral systemic bioavailability, well anti-inflammatory efficacy, of curcumin (Cur) in conditions acute colon kidney inflammation. EK synthesized using straightforward Mannich reaction between EGCG L-lysine (K), resulting formation oligomers. These oligomers spontaneously self-assemble into nanoparticles with spherical morphology an average diameter approximately 160 nm. In vitro studies reveal that exhibit remarkable radical-scavenging capabilities effectively regulate redox processes within macrophages, key component body’s inflammatory response. By efficiently encapsulating nanoparticles, create Cur@EK, formulation demonstrates synergistic effect. Specifically, Cur@EK significantly reduces levels pro-inflammatory cytokines TNF-α IL-6 while increasing cytokine IL-10 lipopolysaccharide-stimulated highlighting its potent properties. When administered either orally or intravenously, shows superior compared free exhibits pronounced effects mouse models ulcerative colitis injury. findings suggest platform not only enhances but also amplifies impact, offering new avenue for treatment management inflammation both contexts.

Язык: Английский

E. coli as a Smart Thermo-Vector for Combating Solid Tumors: A Synergistic Heat-Induced Cancer Therapy Approach DOI
Tashmeen Kaur,

Neeta Devi,

Deepika Sharma

и другие.

Bioconjugate Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 19, 2025

Heat-induced cancer therapies such as magnetic hyperthermia-based therapy (MHCT) and photothermal tumor ablation (PTT) have garnered significant attention minimally invasive new-generation modalities. However, solid tumors associated with hypoxia present a considerable challenge to effective therapy. In this study, we took up the of mitigating limiting penetration ability nanoparticles by integrating polydopamine-coated motile anaerobic bacteria (PDBs) function smart thermo-vector. The developed PDBs are capable self-navigating hypoxic thermo-therapy agents induce heat through exposure an alternating field or near-infrared laser light. thermo-vector system exhibited dual-functioning synergistic antitumor effect MHCT PTT outstanding targeting efficiency, outperforming conventional 'nanoparticles only' approach. heat-induced cellular oxidative stress disrupted mitochondrial led 80% cytotoxicity within 24 h treatment. PDB-based approach complete regression in c57BL/6 mice 21 days treatment tumor-free survival for 60 without recurrence, proving capability combatting tumors.

Язык: Английский

Процитировано

0
Immunomodulatory All-Natural Kelp Decellularized Scaffold Prepared Using Deep Eutectic Solvent with Angiogenic Properties for Accelerating Diabetic Wound Healing DOI

Ruyi Ren,

Tiange Zhao, Luxi Li

и другие.

ACS Biomaterials Science & Engineering, Год журнала: 2025, Номер unknown

Опубликована: Март 20, 2025

Excessive oxidative stress, chronic inflammation, and impaired vascularization are the main barriers to diabetic wound repair. A decellularized extracellular matrix (dECM) with a native ECM structure is promising biomaterial candidate for healing. However, traditional decellularization process (reagents) can diminish structural stability, mechanical properties, bioactive components of dECM. To address these issues, we developed an intrinsically kelp scaffold (Im-Gly2) using natural gentle deep eutectic solvents (DES) accelerating Im-Gly2 had stable porous 3D (80.7 μm) suitable which could support cell growth, proliferation, migration. Due retention fucoidan, polyphenols (735.3 μg/g), flavonoids, demonstrated intrinsic antioxidant immunomodulatory effects. It effectively reduced reactive oxygen species (ROS) production in RAW264.7 macrophages promoted their differentiation into M2 phenotype. Notably, tube formation through paracrine mechanisms by inducing expression transforming proliferative cytokines from macrophage. In vivo, accelerated healing wounds alleviating angiogenesis, granulation tissue formation, collagen deposition, re-epithelialization. Taken together, our study provides novel strategy fabricating dECM without cross-linking exogenous substances

Язык: Английский

Процитировано

0
Next-gen ferulic acid-stabilized gold nanoparticles: exploring their sensing capabilities and therapeutic efficacy DOI Creative Commons

Atiya Kaleem,

Aisha Sana,

Rafia Usman Khan

и другие.

Therapeutic Delivery, Год журнала: 2025, Номер unknown, С. 1 - 11

Опубликована: Март 20, 2025

Aim This study focuses on the synthesis and evaluation of innovative gold nanoparticles (AuNps) stabilized by short-chain ferulic acid (FA), specifically 4-hydroxy-3-methoxy-cinnamic acid.

Язык: Английский

Процитировано

0
Bone marrow vessels are hyperpermeable to macromolecules and nanoscale medicine in a size-dependent manner DOI Creative Commons
John D. Martin, Kazuko Toh,

Margaret R. Martin

и другие.

Journal of Controlled Release, Год журнала: 2025, Номер unknown, С. 113669 - 113669

Опубликована: Март 1, 2025

Bone marrow (BM) has roles in health and disease, so systemically administered nanocarriers (NCs) targeting or avoiding BM are desirable. While the hydrodynamic diameter of NCs can be tuned to target avoid various organs, size dependence extravasation from vessels is unknown. To clarify passive transvascular transport BM, we performed vessel permeability measurements murine calvaria using confocal fluorescent microscopy with fluorescently labeled dextrans, albumin, polymeric micelles as model probes. Unexpectedly, found macromolecules decreases increasing between 4 nm 32 nm. We modeled this data mathematical models predict an effective pore for sinusoids 47 non-sinusoids 37 nm, estimated maximum sizes 61 53 respectively. Finally, tested these predictions by demonstrating that 70 micelles, which larger than size, hindered relative 30 micelles. These results establish design criteria controlling NC towards modulating delivery BM.

Язык: Английский

Процитировано

0
Curcumin-incorporated EGCG-based nano-antioxidants alleviate colon and kidney inflammation via antioxidant and anti-inflammatory therapy DOI Creative Commons
Qingqing Pan, Li Xie, Zhu Huang

и другие.

Regenerative Biomaterials, Год журнала: 2024, Номер 11

Опубликована: Янв. 1, 2024

Abstract Natural remedies are gaining attention as promising approaches to alleviating inflammation, yet their full potential is often limited by challenges such poor bioavailability and suboptimal therapeutic effects. To overcome these limitations, we have developed a novel nano-antioxidant (EK) based on epigallocatechin gallate (EGCG) aimed at enhancing the oral systemic bioavailability, well anti-inflammatory efficacy, of curcumin (Cur) in conditions acute colon kidney inflammation. EK synthesized using straightforward Mannich reaction between EGCG L-lysine (K), resulting formation oligomers. These oligomers spontaneously self-assemble into nanoparticles with spherical morphology an average diameter approximately 160 nm. In vitro studies reveal that exhibit remarkable radical-scavenging capabilities effectively regulate redox processes within macrophages, key component body’s inflammatory response. By efficiently encapsulating nanoparticles, create Cur@EK, formulation demonstrates synergistic effect. Specifically, Cur@EK significantly reduces levels pro-inflammatory cytokines TNF-α IL-6 while increasing cytokine IL-10 lipopolysaccharide-stimulated highlighting its potent properties. When administered either orally or intravenously, shows superior compared free exhibits pronounced effects mouse models ulcerative colitis injury. findings suggest platform not only enhances but also amplifies impact, offering new avenue for treatment management inflammation both contexts.

Язык: Английский

Процитировано

4