Ginseng Stem-and-Leaf Saponins Mitigate Chlorpyrifos-Evoked Intestinal Toxicity In Vivo and In Vitro: Oxidative Stress, Inflammatory Response and Apoptosis DOI Open Access
Silu Liu, Xiaoying Zhu, Hongyan Pei

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15968 - 15968

Опубликована: Ноя. 4, 2023

In recent years, the phenomenon of acute poisoning and organ damage caused by organophosphorus pesticides (OPs) has been a frequent occurrence. Chlorpyrifos (CPF) is one most widely used pesticides. The main active components ginseng stems leaves are total stem-and-leaf saponins (GSLSs), which have various biological effects, including anti-inflammatory, antioxidant anti-tumor activities. We speculate that these could great potential in treatment severe diseases relief organophosphorus-pesticide-induced side effects; however, their mechanism action still unknown. At present, our work aims to evaluate effects GSLSs on antioxidation CPF vivo vitro pharmacological mechanisms. Mice treated with (5 mg/kg) showed intestinal mucosal injury, an elevated diamine oxidase (DAO) index, decreased expression occlusive protein-1 (ZO-1) protein, impaired oxidation system villi relaxation. addition, chlorpyrifos exposure significantly increased contents inflammatory factor TNF-α oxidative-stress-related indicators superoxide dismutase (SOD), catalase (CAT), glutathione SH (GSH), peroxidase (GSH-PX), reactive oxygen species (ROS) capacity (T-AOC); level lipid peroxide malondialdehyde (MDA); reversed Bax caspase; activated NF-κB-related proteins. Interestingly, GSLS supplementation at doses 100 200 mg/kg changes after treatment. Similar results were observed cultured RAW264.7 cells. Using flow cytometry, Hoechst staining (30 μg/mL, 60 μg/mL) improve cell injury apoptosis reduce accumulation ROS conclusion, play protective role against CPF-induced enterotoxicity inhibiting NF-κB-mediated alleviating oxidative stress inflammation.

Язык: Английский

Effects of dietary Astragalus membranaceus and Codonopsis pilosula extracts on growth performance, antioxidant capacity, immune status, and intestinal health in broilers DOI Creative Commons
Shun Liu, Gengsheng Xiao, Qi Wang

и другие.

Frontiers in Veterinary Science, Год журнала: 2023, Номер 10

Опубликована: Дек. 7, 2023

The objective of this study was to examine the effects dietary Chinese herbal medicine (CHM) consisting Astragalus membranaceus (Fisch.) Bunge (AMT) and Codonopsis pilosula (Franch.) Nannf (CPO) extracts on growth performance, antioxidant capacity, immune status, intestinal health broiler chickens. Two groups were formed, each six replicates 12 one-day-old healthy male 817 white feather broilers. Broilers fed either a basal diet (CON group) or supplemented with 500 mg/kg CHM. trial lasted 50 days. results showed that CHM supplementation resulted in enhanced feed efficiency capacity both serum liver, while it reduced uric acid endotoxin levels, as well diamine oxidase activity (p < 0.05). Additionally, treatment increased height jejunum villi upregulated Claudin-1 expression jejunal mucosa accompanied by an increase mRNA levels interleukin-6 (IL-6), interferon-γ (IFN-γ), interferon-β (IFN-β), tumor necrosis factor-α (TNF-α), anti-inflammatory cytokine interleukin-10 (IL-10) presence caused proportions Bacteroidetes unclassified Bacteroidales but led decrease those Firmicutes Alistipes composition microbiota correlated conversion ratio, metabolites, gene based Spearman correlation analysis. findings indicated consumption improved utilization feed, pro-inflammatory cytokines mucosa, decreased level activities lactate dehydrogenase serum, which could potentially be linked changes gut

Язык: Английский

Процитировано

10
Mitochondrial miR-12294-5p-Regulated Copper Exposure-Caused Mitochondrial-Dependent Ferroptosis by Targeted Inhibition of CISD1 in Chicken Hepatocytes DOI

Gaolong Zhong,

Mengran Wang,

Bingxia Sun

и другие.

Journal of Agricultural and Food Chemistry, Год журнала: 2024, Номер 72(28), С. 15948 - 15958

Опубликована: Июль 5, 2024

Copper (Cu) is a common trace element additive in animal and human foods, excessive intake of Cu has been shown to cause hepatotoxicity, but the underlying mechanism remains unclear. Our previous research found that exposure dramatically upregulated mitochondrial miR-12294-5p expression confirmed its targeted inhibition CISD1 chicken hepatocytes. Thus, we aimed explore potential role mitomiR-12294-5p/CISD1 axis exposure-resulted hepatotoxicity. Here, observed resulted accumulation pathological injury livers. Moreover, caused mitochondrial-dependent ferroptosis hepatocytes, which were prominent on increased Fe2+ lipid peroxidation, inhibited levels CISD1, GPX4, DHODH, IDH2, also enhanced level PTGS2. Notably, identified mitomiR-2954 effectively mitigated Cu-exposure-resulted peroxidation prevented development ferroptosis. However, increasing mitomiR-12294-5p considerably aggravated influence these indicators. Meanwhile, overexpression alleviated Cu-caused ferroptosis, while silent eliminated therapeutic inhibitor. Overall, our findings indicated played critical function hepatotoxicity chickens by regulating

Язык: Английский

Процитировано

3
Copper homeostasis and cuproptosis‐related genes: Therapeutic perspectives in non‐alcoholic fatty liver disease DOI Creative Commons
Wangjing Tan, Junli Zhang, Li Chen

и другие.

Diabetes Obesity and Metabolism, Год журнала: 2024, Номер 26(11), С. 4830 - 4845

Опубликована: Сен. 4, 2024

Non-alcoholic fatty liver disease (NAFLD), a metabolic-associated disease, has become the most common chronic worldwide. Recently, discovery of cuproptosis, newly identified mode cell death, further highlighted importance copper in maintaining metabolic homeostasis. An increasing number studies have confirmed that metabolism is closely related to pathogenesis NAFLD. However, relationship between NAFLD and metabolism, especially remains unclear. In this review, we aim summarize current understanding its dysregulation, particularly role dysregulation More importantly, review emphasizes potential gene-targeted therapeutic strategies, challenges future cuproptosis-related genes treatment This aims provide innovative strategies for

Язык: Английский

Процитировано

3
Using heat shock protein (HSP) inducers as an approach to increase the viability of sterlet (Pisces; Acipenseridae; Acipenser ruthenus) cells against environmental diazinon toxicity DOI
Sevda Zarei, Hossein Ghafouri,

Leila Vahdatiraad

и другие.

Journal of Hazardous Materials, Год журнала: 2023, Номер 465, С. 133194 - 133194

Опубликована: Дек. 7, 2023

Язык: Английский

Процитировано

8
Ginseng Stem-and-Leaf Saponins Mitigate Chlorpyrifos-Evoked Intestinal Toxicity In Vivo and In Vitro: Oxidative Stress, Inflammatory Response and Apoptosis DOI Open Access
Silu Liu, Xiaoying Zhu, Hongyan Pei

и другие.

International Journal of Molecular Sciences, Год журнала: 2023, Номер 24(21), С. 15968 - 15968

Опубликована: Ноя. 4, 2023

In recent years, the phenomenon of acute poisoning and organ damage caused by organophosphorus pesticides (OPs) has been a frequent occurrence. Chlorpyrifos (CPF) is one most widely used pesticides. The main active components ginseng stems leaves are total stem-and-leaf saponins (GSLSs), which have various biological effects, including anti-inflammatory, antioxidant anti-tumor activities. We speculate that these could great potential in treatment severe diseases relief organophosphorus-pesticide-induced side effects; however, their mechanism action still unknown. At present, our work aims to evaluate effects GSLSs on antioxidation CPF vivo vitro pharmacological mechanisms. Mice treated with (5 mg/kg) showed intestinal mucosal injury, an elevated diamine oxidase (DAO) index, decreased expression occlusive protein-1 (ZO-1) protein, impaired oxidation system villi relaxation. addition, chlorpyrifos exposure significantly increased contents inflammatory factor TNF-α oxidative-stress-related indicators superoxide dismutase (SOD), catalase (CAT), glutathione SH (GSH), peroxidase (GSH-PX), reactive oxygen species (ROS) capacity (T-AOC); level lipid peroxide malondialdehyde (MDA); reversed Bax caspase; activated NF-κB-related proteins. Interestingly, GSLS supplementation at doses 100 200 mg/kg changes after treatment. Similar results were observed cultured RAW264.7 cells. Using flow cytometry, Hoechst staining (30 μg/mL, 60 μg/mL) improve cell injury apoptosis reduce accumulation ROS conclusion, play protective role against CPF-induced enterotoxicity inhibiting NF-κB-mediated alleviating oxidative stress inflammation.

Язык: Английский

Процитировано

7