Development of Biomimetic Substrates for Limbal Epithelial Stem Cells Using Collagen-Based Films, Hyaluronic Acid, Immortalized Cells, and Macromolecular Crowding DOI Creative Commons
Mehmet Gürdal, Gülinnaz Ercan, Özlem Barut Selver

и другие.

Life, Год журнала: 2024, Номер 14(12), С. 1552 - 1552

Опубликована: Ноя. 26, 2024

Despite the promising potential of cell-based therapies developed using tissue engineering techniques to treat a wide range diseases, including limbal stem cell deficiency (LSCD), which leads corneal blindness, their commercialization remains constrained. This is primarily attributable limited sources, use non-standardizable, unscalable, and unsustainable techniques, extended manufacturing processes required produce transplantable tissue-like surrogates. Herein, we present first demonstration novel approach combining collagen films (CF), hyaluronic acid (HA), human telomerase-immortalized epithelial cells (T-LESCs), macromolecular crowding (MMC) develop innovative biomimetic substrates for (LESCs). The initial step involved fabrication characterization CF enriched with HA (CF-HA). Subsequently, T-LESCs were seeded on CF, CF-HA, culture plastic (TCP). Thereafter, effect these matrices basic cellular function tissue-specific extracellular matrix (ECM) deposition or without MMC was evaluated. viability metabolic activity cultured TCP found be similar, while CF-HA induced highest (p < 0.05) proliferation. It notable that growth, whereas increased IV, fibronectin, laminin in T-LESC culture. data highlight of, particular, immortalized development substrates, could utilized ocular surface reconstruction following further vitro, vivo, clinical validation approach.

Язык: Английский

Macromolecular Crowding Supports the Generation of Basal Membrane-Rich Pericyte-Based Cell Sheets Useful for Cell Therapy of Diabetic Wounds DOI Creative Commons
Andrea Rampin,

C. Caravaggi,

Luigi Troisi

и другие.

Cell Transplantation, Год журнала: 2025, Номер 34

Опубликована: Март 1, 2025

Diabetic foot ulcers (DFUs) are associated with a high risk of amputations and 50% 5-year survival rate due at least in part to the limited angiogenic wound healing capacity patients diabetes. Cell therapy via intramuscular injection peripheral blood mononuclear cells showed encouraging but results. Such limitations may arise from ability therapeutic adhere target tissue. The development methodology able support targeted delivery viable would improve cell outcomes DFU. Here, we optimized protocol for production autologous extracellular matrix (ECM)-rich pericyte-based sheets delivery. Pericytes were isolated skeletal muscle biopsies DFU non-diabetic controls characterized by flow cytometry immunofluorescence. Human umbilical vein endothelial used optimization collagen IV deposition positive correlation seeding density negative one sub-culture passaging ( P < 0.05). Macromolecular crowding significantly increased both human patient-derived pericytes 0.01) without affecting proliferation > Finally, effectively deposited ECM supporting their use sheet production.

Язык: Английский

Процитировано

0
Underestimated role of macromolecular crowding in bioengineered in vitro models of health and diseases DOI Creative Commons
Jérémy Lagrange,

G. Velde,

Patrick Lacolley

и другие.

Materials Today Bio, Год журнала: 2025, Номер unknown, С. 101772 - 101772

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Development of Biomimetic Substrates for Limbal Epithelial Stem Cells Using Collagen-Based Films, Hyaluronic Acid, Immortalized Cells, and Macromolecular Crowding DOI Creative Commons
Mehmet Gürdal, Gülinnaz Ercan, Özlem Barut Selver

и другие.

Life, Год журнала: 2024, Номер 14(12), С. 1552 - 1552

Опубликована: Ноя. 26, 2024

Despite the promising potential of cell-based therapies developed using tissue engineering techniques to treat a wide range diseases, including limbal stem cell deficiency (LSCD), which leads corneal blindness, their commercialization remains constrained. This is primarily attributable limited sources, use non-standardizable, unscalable, and unsustainable techniques, extended manufacturing processes required produce transplantable tissue-like surrogates. Herein, we present first demonstration novel approach combining collagen films (CF), hyaluronic acid (HA), human telomerase-immortalized epithelial cells (T-LESCs), macromolecular crowding (MMC) develop innovative biomimetic substrates for (LESCs). The initial step involved fabrication characterization CF enriched with HA (CF-HA). Subsequently, T-LESCs were seeded on CF, CF-HA, culture plastic (TCP). Thereafter, effect these matrices basic cellular function tissue-specific extracellular matrix (ECM) deposition or without MMC was evaluated. viability metabolic activity cultured TCP found be similar, while CF-HA induced highest (p < 0.05) proliferation. It notable that growth, whereas increased IV, fibronectin, laminin in T-LESC culture. data highlight of, particular, immortalized development substrates, could utilized ocular surface reconstruction following further vitro, vivo, clinical validation approach.

Язык: Английский

Процитировано

1