Advances in genetics, Год журнала: 2025, Номер unknown, С. 29 - 52
Опубликована: Янв. 1, 2025
Язык: Английский
Seminars in Respiratory and Critical Care Medicine, Год журнала: 2024, Номер 45(04), С. 491 - 502
Опубликована: Авг. 1, 2024
Sepsis, the dysregulated immune response of host to infections, leads numerous complications, including multiple organ dysfunction with sepsis-associated acute kidney injury (SA-AKI) being a frequent complication associated increased risk mortality and progression toward chronic disease (CKD). Several mechanisms have been widely investigated in understanding complex pathophysiology SA-AKI, hemodynamic alterations, inflammation, oxidative stress, direct cellular driven by pathogens or cell-derived products (pathogen-associated molecular patterns damage-associated patterns). Despite advancements management septic patients, prognosis SA-AKI patients remains significantly poor is high in-hospital adverse long-term outcomes. Therefore, recent research has focused on early identification specific endotypes subphenotypes through epigenetic analysis use potential biomarkers, either alone combination clinical data, improve prognosis. Epigenetic regulation, such as DNA methylation, histone modifications, noncoding RNA modulation, crucial modulating gene expression stress renal SA-AKI. At same time, these modifications are dynamic reversible processes that can alter several pathways implicated context response, tolerance status. In addition, may exacerbate damage causing persistent inflammation metabolic reprogramming, leading CKD. This review aims provide comprehensive characteristics define also exploring targeted therapies patient outcomes limit this syndrome.
Язык: Английский
Процитировано
4
Non-Coding RNA, Год журнала: 2024, Номер 10(1), С. 11 - 11
Опубликована: Фев. 5, 2024
Noncoding RNAs (ncRNAs), including circular (circRNAs) and N6-methyladenosine (m6A), have been shown to play a critical role in the development of various diseases obesity metabolic disorder-associated fatty liver disease (MAFLD). Obesity is chronic caused by excessive fat accumulation body, which has recently become more prevalent foremost risk factor for MAFLD. Causes may involve interaction genetic, behavioral, social factors. m6A RNA methylation might add novel inspiration understanding MAFLD with post-transcriptional regulation gene expression. In particular, circRNAs, microRNAs (miRNAs), be implicated progression Interestingly, modification can modulate translation, degradation, other functions ncRNAs. miRNAs/circRNAs also modifications affecting writers, erasers, readers. turn, ncRNAs could expression regulators different ways. However, there limited evidence on how these interact affect promotion diseases. It seems that occur DNA, RNA, proteins associated several biological properties. This study provides mechanistic association diseases, especially Comprehension between contribute treatment tactics
Язык: Английский
Процитировано
3
Epigenetics, Год журнала: 2024, Номер 19(1)
Опубликована: Май 9, 2024
Some benign and malignant breast tumours are similar in pathological morphology, which difficult to be distinguished clinical diagnosis. In this study, we intended explore novel biomarkers for differential diagnosis of tumours. Methylation EPIC 850K beadchip RNA-sequencing were used analyse 29 tissue samples from patients with early-stage cancer (BC) differently methylated expressed genes. The altered methylation IL21R was semi-quantitatively validated an independent study 566 (279 BC vs. 287 tumours) using mass spectrometry. Binary logistic regression analysis performed evaluate the association between BC. BC-associated hypomethylation overexpression identified discovery round. validation round, presented significant compared women (ORs ≥1.29 per-10% methylation, p-values ≤ 5.69E–14), even enhanced ER-negative PR-negative as well triple-negative showed efficient discriminatory power distinguish (area under curve (AUC) = 0.88), especially (AUC 0.95), 0.93) 0.96). We disclosed tumours, revealed somatic change DNA could a potential biomarker molecular pathology
Язык: Английский
Процитировано
3
Biomolecules, Год журнала: 2024, Номер 14(8), С. 926 - 926
Опубликована: Июль 30, 2024
Enkephalins, a subclass of endogenous opioid peptides, play pivotal role in pain modulation. Enkephalins primarily exert their effects through receptors located widely throughout both the central and peripheral nervous systems. This review will explore mechanisms by which enkephalins produce analgesia, emotional regulation, neuroprotection, other physiological effects. Furthermore, this analyze involvement modulation different pathologies characterized severe pain. Understanding complex processing provides valuable insight into potential therapeutic strategies for managing disorders.
Язык: Английский
Процитировано
3
Ageing Research Reviews, Год журнала: 2024, Номер 102, С. 102556 - 102556
Опубликована: Окт. 30, 2024
Язык: Английский
Процитировано
3
International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11801 - 11801
Опубликована: Ноя. 2, 2024
Tuberculosis caused by the obligate intracellular pathogen, Mycobacterium tuberculosis, is one among prime causes of death worldwide. An urgent remedy against tuberculosis paramount importance in current scenario. However, complex nature this appalling disease contributes to limitations existing medications. The quest for better treatment approaches driving research field host epigenomics forward context with tuberculosis. interplay between various epigenetic factors and pathogen under investigation. A comprehensive understanding how orchestrates such favors its survival within increasing demand. modifications beneficial are reversible possess potential be targets therapeutic approaches. mechanisms, including histone modifications, DNA methylation, miRNA modification, being explored their impact on pathogenesis. In article, we deciphering role mycobacterial regulators strategies like cytokine expression, macrophage polarization, autophagy, apoptosis, along a glimpse host-directed therapies.
Язык: Английский
Процитировано
3
Analytical Chemistry, Год журнала: 2025, Номер unknown
Опубликована: Янв. 13, 2025
Accurate identification and quantification of 5-hydroxymethylcytosine (5hmC) can help elucidate its function in gene expression disease pathogenesis. Current 5hmC analysis methods still present challenges, especially for clinical applications, such as having a risk false-positive results lack sufficient sensitivity. Herein, method fragment-specific DNA sequences with extreme specificity, high sensitivity, applicability was established using quantitative real-time PCR (qPCR)-based workflow through the combination enzymatic digestion biological deamination strategy (EDD-5hmC assay). The EDD-5hmC approach enriched glycosylated via enzyme then APOBEC (apolipoprotein B mRNA editing catalytic polypeptide-like)-mediated to efficiently differentiate between various cytosine(C) modification states, resulting specificity that nonspecific amplification is reduced over eight million-fold. Moreover, nondestructive treatment process assay exhibits yielding limit detection 30 aM. For first time, we measured levels colorectal cancer tissues matched paracancerous evaluate ability cancer, area under receiver operating characteristic curve up 82.8% single Septin9 83.6% combinations Syndecan-2 (SDC2), demonstrating promising accurately quantifying level.
Язык: Английский
Процитировано
0
Asian Journal of Biological Sciences, Год журнала: 2025, Номер 18(2), С. 516 - 532
Опубликована: Янв. 23, 2025
Язык: Английский
Процитировано
0
Neuroglia, Год журнала: 2025, Номер 6(1), С. 11 - 11
Опубликована: Март 1, 2025
Background/Objectives: Autism Spectrum Disorder (ASD) is a complex neurodevelopmental condition marked by challenges in social communication, restricted interests, and repetitive behaviors. Recent studies highlight the crucial roles of neuroglial cells—astrocytes, microglia, oligodendrocytes—in synaptic function, neural connectivity, neuroinflammation. These findings offer fresh perspective on ASD pathophysiology. This review synthesizes current knowledge dysfunction ASD, emphasizing its role pathophysiological mechanisms, genetic influences, potential therapeutic strategies. Methods: We conducted comprehensive literature review, integrating insights from neuroscience, molecular biology, clinical studies. Special focus was given to glial-mediated neuroinflammatory plasticity regulation, impact mutations signaling homeostasis. Results: Neuroglial evident abnormal pruning impaired astrocytic glutamate defective oligodendrocyte-driven myelination, which collectively disrupt neuronal architecture. Emerging therapies targeting these pathways, including anti-inflammatory drugs, microglial modulators, cell-based approaches, show promise alleviating key symptoms. Additionally, advanced interventions such as gene editing glial progenitor therapy present opportunities correct underlying dysfunction. Conclusions: establishes framework for understanding contributions ASD. By diverse disciplines, it enhances our pathophysiology paves way novel strategies pathways.
Язык: Английский
Процитировано
0
Journal of Chemical Information and Modeling, Год журнала: 2025, Номер unknown
Опубликована: Март 13, 2025
Synonymous mutations, once considered to be biologically neutral, are now recognized affect protein expression and function by altering the RNA splicing, stability, or translation efficiency. These effects can contribute disease, making prediction of pathogenicity a crucial task. Computational methods have been developed analyze sequence features biological functions synonymous but existing face limitations, including scarcity labeled data, reliance on other tools, insufficient representation feature interrelationships. Here, we present FDPSM, novel method specifically designed predict pathogenic mutations. FDPSM was trained robust data set 4251 positive negative training samples enhance predictive accuracy. The leveraged comprehensive features, genomic context, conservation, splicing effects, functional epigenomics, without relying scores from mutation tools. Recognizing that original alone may not fully capture distinctions between benign enhanced extracting effective information interactions distribution these features. experimental results showed significantly outperformed in predicting offering more accurate reliable tool for this important is available at https://github.com/xialab-ahu/FDPSM.
Язык: Английский
Процитировано
0