Synthesis, Год журнала: 2024, Номер unknown
Опубликована: Дек. 17, 2024
Abstract The pentasaccharide repeating unit containing 6-deoxy-l-talose corresponding to the cell wall O-polysaccharide of Escherichia coli (E. coli) O66 strain has been successfully synthesized in very good yield using sequential glycosylations judiciously functionalized monosaccharide intermediates. Incorporation β-d-mannosidic linkage and 6-deoxy-α-l-talose was achieved excellent yield.
Язык: Английский
Vaccines, Год журнала: 2024, Номер 12(11), С. 1290 - 1290
Опубликована: Ноя. 19, 2024
Glycoconjugate vaccines have been effective in preventing numerous bacterial infectious diseases and shown recent potential to treat cancers through active immunotherapy. Soluble polysaccharides elicit short-lasting immune responses are usually covalently linked immunogenic carrier proteins enhance the antigen-specific response by stimulating T-cell-dependent mechanisms. Nonetheless, conjugation of purified complexifies vaccine production, immunization with protein glycoconjugates can lead undesirable interference carrier. Recently, use nanoparticles nanoassemblies for delivery antigenic saccharides has gathered attention from scientific community. Nanoparticles be easily functionalized a diversity functionalities, including T-cell epitope, immunomodulator synthetic saccharides, allowing modulation polarization glycoantigen-specific response. Notably, glycan protects antigens degradation enhances their uptake cells. Different types nanoparticles, such as liposomes assembled lipids, inorganic virus-like particles dendrimers, explored glycovaccine design. The versatility ability induce robust make them attractive platforms saccharides. present review aims at summarizing advancements nano-scaled systems glycoantigens. After briefly presenting immunological mechanisms required promote against this will offer an overview current trends nanoparticle-based
Язык: Английский
Процитировано
1
Advanced Synthesis & Catalysis, Год журнала: 2023, Номер 365(14), С. 2344 - 2349
Опубликована: Июнь 21, 2023
Abstract Accessing homogenous glycoconjugates is of significance for assessing their biophysical activities and sometimes as vaccine candidate. Synthesis involves two species which, glycosyl donor plays pivotal role in controlling the outcome glycosylation. Recently discovered alkynyl carbonate donors possess self‐stability, high reactivity, fast reaction time (15–30 min) when activated with [Au]/[Ag]‐catalysts. Herein, we report metal free conditions activation same using I 2 /TMSOTf stoichiometric quantities. Extrusion product was characterized to be vinylidene iodide by single crystal X‐ray analysis. The were illustrated suitable synthesis various glycosides, purine/pyrimidine nucleosides a pentasaccharide repeating unit Klebsiella pneumoniae (O‐3‐antigen).
Язык: Английский
Процитировано
3
Journal of Agricultural and Food Chemistry, Год журнала: 2023, Номер 71(37), С. 13857 - 13868
Опубликована: Сен. 9, 2023
Colanic acid (CA) is a natural polysaccharide macromolecule with rich and unique biological properties promising candidate for use in food cosmetics. To date, the efficient biosynthesis of CA influence product accumulation on strains used have yet to be precisely investigated. Herein, bottlenecks metabolic pathway were untangled by finely regulating expression manA, cpsG, fcl, rcsA. Engineered produced at >1 g/L shake flasks without dependence cold temperatures, it was verified 1 L bioreactor titer up 18.64 within 24 h. The caused decrease saturated fatty content (represented C16:0 C18:0) cell membrane. This study demonstrated engineering production factories provided insights into barriers solutions faced products.
Язык: Английский
Процитировано
3
Molecules, Год журнала: 2023, Номер 28(15), С. 5644 - 5644
Опубликована: Июль 25, 2023
Controlling the stereoselectivity of 1,2-
Язык: Английский
Процитировано
2
Synthesis, Год журнала: 2024, Номер unknown
Опубликована: Дек. 17, 2024
Abstract The pentasaccharide repeating unit containing 6-deoxy-l-talose corresponding to the cell wall O-polysaccharide of Escherichia coli (E. coli) O66 strain has been successfully synthesized in very good yield using sequential glycosylations judiciously functionalized monosaccharide intermediates. Incorporation β-d-mannosidic linkage and 6-deoxy-α-l-talose was achieved excellent yield.
Язык: Английский
Процитировано
0