ACS Applied Polymer Materials,
Год журнала:
2024,
Номер
6(16), С. 9859 - 9865
Опубликована: Авг. 13, 2024
In
classical
reversible
addition
fragmentation
chain
transfer
(RAFT)
polymerization,
the
use
of
sulfur-containing
reagents
is
required,
which
inevitably
causes
some
pollution
to
environment.
From
perspective
green
chemistry,
nontoxic
and
harmless
reactants
does
not
pollute
environment
builds
environmentally
friendly
biosensors.
this
study,
we
proposed
a
sulfur-free
RAFT
electrochemical
biosensor
without
transition
metal
or
sulfur
catalyst
using
protein
kinase
A
(PKA)
as
model
analyte.
sensing
process,
peptide
consisting
kinase-specific
recognition
sequence
was
designed.
The
can
be
attached
negatively
charged
surface
an
APTMS-modified
indium
tin
oxide
(ITO)
electrode
(APTMS/ITO)
via
amide
reaction.
When
PKA
present,
substrate
phosphorylated,
then
immediately
coordinated
with
Zr4+.
Then,
Zr4+
used
linker
adsorb
carboxyl-modified
hollow
gold
nanoparticles
(HAuNPs)
surface.
SH-β-CD@2-NH2-Ada
inclusion
complex
conjugated
HAuNPs
Au–S,
immersed
into
polymerization
solution
catalytic
(CCTP)
RAFT,
resulting
in
increased
signal.
platform
offers
advantages
simplicity,
ease
handling,
enhanced
phosphorylation,
thanks
emulsion
need
for
modification
immobilization
processes.
Also,
method
friendly,
promises
promising
results,
represents
major
advance
development
macromolecular
preparation.
Basically,
no
toxic
dangerous
chemical
are
entire
biomolecular
detection
process.
Moreover,
given
pivotal
role
cell
lysate
inhibition
activity,
demonstrates
prospects
kinase-related
bioanalysis
clinical
biomedicine.
International Journal of Molecular Sciences,
Год журнала:
2023,
Номер
24(24), С. 17360 - 17360
Опубликована: Дек. 11, 2023
TIMAP
(TGF-β-inhibited
membrane
associated
protein)
is
abundant
in
endothelial
cells,
and
it
has
been
regarded
as
a
member
of
the
myosin
phosphatase
targeting
protein
(MYPT)
family.
Our
workgroup
previously
identified
several
interacting
partners
proved
its
regulatory
subunit
role
for
1
catalytic
(PP1c).
also
expressed
neuronal
but
details
function
have
not
studied
yet.
Therefore,
we
aimed
to
explore
especially
during
differentiation.
Expression
was
both
at
mRNA
levels
SH-SY5Y
human
neuroblastoma
cells.
Differentiation
cells
optimized
by
detection
differentiation
markers,
such
β3-tubulin,
nestin
inhibitor
(ID1)
using
qPCR
Western
blot.
We
found
downregulation
In
accordance
with
this,
overexpression
recombinant
attenuated
Moreover,
subcellular
localization
changed
translocated
from
plasma
into
nucleus.
The
nuclear
interactome
revealed
more
than
50
proteins,
offering
possibility
further
investigate
key
physiological
pathways
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Июль 11, 2024
Abstract
A
novel
electrochemiluminescence
(ECL)
method
was
developed
for
analyzing
protein
kinase
(PKA)
ultra-sensitively
based
on
amidated
nano-titanium
(NH2-TiO2)
embellished
carbon
dots
(Mg@N-CDs)
fluorescent
probe,
which
integrated
the
target
recognition
and
ECL-signal
enhancement.
The
Cys-labeled
kemptides
were
employed
to
build
a
serine-rich
synthetic
substrate-heptapeptide
(Cys-kemptide)
Au-electrode
surface.
Then,
PKA-induced
biosensor
triggered
as
signal
switch
introduce
large
amounts
of
TiO2
decorated
Mg@N-CDs
nanohybrid
(Ti@NMg-CDs)
into
AuE/Cys-phosphopeptides
output.
In
particular,
presence
PKA
could
induce
formation
Cys-phosphopeptides
by
catalytic
reaction
between
specific
substrate
(kemptide)
PKA,
act
an
initiator
link
Ti@NMg-CDs
according
bridge
interactions
Ti-O-P.
By
this
way,
multiple
adsorbed
onto
single
not
only
provided
high
selectivity
but
also
surface
area,
well
unprecedented
ECL
efficiency.
Using
enhanced
sensor,
limit-of-detection
4.89
×
10−
4
U/mL
(S/N
=
3).
proposed
universally
applicable
screening
inhibitors
determining
other
kinases
activity.
Our
sensing-system
has
excellent
performance
specificity
inhibitors,
it
will
inspire
future
effort
clinical
diagnostics
new
drugs
discovery.
ACS Applied Polymer Materials,
Год журнала:
2024,
Номер
6(16), С. 9859 - 9865
Опубликована: Авг. 13, 2024
In
classical
reversible
addition
fragmentation
chain
transfer
(RAFT)
polymerization,
the
use
of
sulfur-containing
reagents
is
required,
which
inevitably
causes
some
pollution
to
environment.
From
perspective
green
chemistry,
nontoxic
and
harmless
reactants
does
not
pollute
environment
builds
environmentally
friendly
biosensors.
this
study,
we
proposed
a
sulfur-free
RAFT
electrochemical
biosensor
without
transition
metal
or
sulfur
catalyst
using
protein
kinase
A
(PKA)
as
model
analyte.
sensing
process,
peptide
consisting
kinase-specific
recognition
sequence
was
designed.
The
can
be
attached
negatively
charged
surface
an
APTMS-modified
indium
tin
oxide
(ITO)
electrode
(APTMS/ITO)
via
amide
reaction.
When
PKA
present,
substrate
phosphorylated,
then
immediately
coordinated
with
Zr4+.
Then,
Zr4+
used
linker
adsorb
carboxyl-modified
hollow
gold
nanoparticles
(HAuNPs)
surface.
SH-β-CD@2-NH2-Ada
inclusion
complex
conjugated
HAuNPs
Au–S,
immersed
into
polymerization
solution
catalytic
(CCTP)
RAFT,
resulting
in
increased
signal.
platform
offers
advantages
simplicity,
ease
handling,
enhanced
phosphorylation,
thanks
emulsion
need
for
modification
immobilization
processes.
Also,
method
friendly,
promises
promising
results,
represents
major
advance
development
macromolecular
preparation.
Basically,
no
toxic
dangerous
chemical
are
entire
biomolecular
detection
process.
Moreover,
given
pivotal
role
cell
lysate
inhibition
activity,
demonstrates
prospects
kinase-related
bioanalysis
clinical
biomedicine.
