Heme Oxygenase-1 as Crucial Biomarker for Detecting Oxidative Stress and Some Parameters in a Sample of Obese Patients with and without Diabetes DOI Creative Commons

Mays Mohammed Abdullah,

Fayhaa Muqdad Khaleel

Clinical Diabetology, Год журнала: 2024, Номер 13(6), С. 349 - 357

Опубликована: Дек. 20, 2024

Objective: Obesity is a significant contributor to various metabolic disorders, including type 2 diabetes (T2D), resulting in heightened oxidative stress. This study aims examine the levels of heme oxygenase-1 (HO-1), their correlation with high-density lipoprotein (HDL) concentration, and influence on onset T2D obese individuals diabetes. Materials methods: The comprised 150 samples categorized into 3 groups, each group further subdivided subgroups: males females aged 30 65 years. Samples were collected at AL-Kindy Teaching Hospital. All sample variables for fasting subjects every assessed. colorimetric approach was employed biochemical assays, encompassing blood sugar (FBS) lipid profiles. Insulin, HO-1, dipeptidyl peptidase-4 (DPP-4) also quantified using ELISA. Subsequently, we statistical analysis elucidate results. Results: Compared obesity group, HO-1 HDL concentrations higher [(18.0 ± 0.40), (39.86 1.26) vs. (10.41 0.74), (36.27 0.85), (ng/mL), (mg/dL), respectively]. showed insulin resistance compared control groups [(4.19 0.874) (1.21 0.39), (0.74 0.142)]. male had elevated result that applied females. Conclusions: enzyme than group. We found positive association between increased may serve as biomarker predict development or other diseases.

Язык: Английский

Unveiling anti-diabetic potential of new thiazole-sulfonamide derivatives: Design, synthesis, in vitro bio-evaluation targeting DPP-4, α-glucosidase, and α-amylase with in-silico ADMET and docking simulation DOI
Hamdy Khamees Thabet, Yousry A. Ammar, Mohd Imran

и другие.

Bioorganic Chemistry, Год журнала: 2024, Номер 151, С. 107671 - 107671

Опубликована: Июль 23, 2024

Язык: Английский

Процитировано

9
Fatty acid conjugated BimBH3 analogues with d‑amino acid substitution as PTPN1 inhibitors with enhanced activity, biostability and orally available potency for the treatment of diabetes DOI

Liyan Gong,

Dong Guo-zhen,

Qianqian Zhang

и другие.

Bioorganic & Medicinal Chemistry, Год журнала: 2025, Номер 121, С. 118107 - 118107

Опубликована: Фев. 8, 2025

Язык: Английский

Процитировано

0
Peptide Therapeutics: Current Status and Future Opportunity with Focus on Nose-to-Brain Delivery DOI
Eva‐Maria Jülke, Annette G. Beck‐Sickinger

Peptides, Год журнала: 2025, Номер unknown, С. 171404 - 171404

Опубликована: Апрель 1, 2025

Язык: Английский

Процитировано

0
Unveiling the medicinal diversity of benzoic acid containing molecules: Insights on druggable targets for type 2 diabetes mellitus DOI

K. Sandhya Rani,

Preety Kumari,

Anita Kumari

и другие.

Bioorganic & Medicinal Chemistry, Год журнала: 2025, Номер 128, С. 118245 - 118245

Опубликована: Май 22, 2025

Язык: Английский

Процитировано

0
Screening and Validation: AI-Aided Discovery of Dipeptidyl Peptidase-4 Inhibitory Peptides from Hydrolyzed Rice Proteins DOI Creative Commons
Cheng Cheng,

Huizi Cui,

Xiangyu Yu

и другие.

Foods, Год журнала: 2025, Номер 14(11), С. 1916 - 1916

Опубликована: Май 28, 2025

Dipeptidyl peptidase-4 (DPP-4) inhibitors play a critical role in the management of type 2 diabetes; however, some synthetic drugs may cause adverse effects. Natural peptides derived from rice offer promising alternative due to their favorable biocompatibility and development potential. In this study, an AI-assisted virtual screening pipeline integrating machine learning, molecular docking, dynamics (MD) simulations was established identify evaluate rice-derived DPP-4 inhibitory peptides. A random forest classification model achieved 85.37% accuracy predicting activity. Peptides generated by simulated enzymatic hydrolysis were screened based on learning docking scores, four proline-rich (PPPPPPPPA, PPPSPPPV, PPPPPY, CPPPPAAY) selected for MD analysis. The simulation results showed that PPPSPPPV formed stable complex with catalytic triad (Ser592–Asp670–His702) through electrostatic hydrophobic interactions, low structural fluctuation (RMSF < 1.75 Å). vitro assays revealed PPPPPY exhibited strongest activity (IC50 = 153.2 ± 5.7 μM), followed PPPPPPPPA (177.0 6.0 μM) (216.3 4.5 μM). This study presents efficient approach combining experimental validation, offering mechanistic foundation natural as candidates functional foods or adjunct diabetes therapies.

Язык: Английский

Процитировано

0
Angular-Substituted [1,4]Thiazino[3,4-a]Isoquinolines: Biological Evaluation and In Silico Studies on DPP-IV Inhibition DOI Open Access
Aleksandar Pashev, Valentin Petrov,

Aleksandrina Pesheva

и другие.

International Journal of Molecular Sciences, Год журнала: 2024, Номер 25(21), С. 11753 - 11753

Опубликована: Ноя. 1, 2024

Recent studies have discovered that aryl-substituted pyrido[2,1-a]isoquinolines the potential to be highly active DPP IV inhibitors. In previous studies, we reported a novel synthetic approach for construction of their sulfur-containing bioisosteric [1,4]thiazino[3,4-a]isoquinolines analogues, incorporating an additional aryl substituent. The present study aims investigate inhibitory activity and cytotoxicity synthesized molecules by in vitro assay. geometry optimization molecular docking compounds were used determine binding modes site IV. analysis revealed energy-minimized poses studied are close most important selectivity cliffs inhibition, forming hydrogen bonds hydrophobic interactions with them. These results can considered as preliminary step towards further structural modifications.

Язык: Английский

Процитировано

2
Enzymatic hydrolysis of buffalo casein enhances DPP-4 inhibition: structural modifications and bioactive peptide identification DOI Creative Commons
Ning An, Jing Yang, Yu Zhang

и другие.

Journal of Dairy Science, Год журнала: 2024, Номер unknown

Опубликована: Ноя. 1, 2024

Dipeptidyl peptidase-4 (DPP-4), the enzyme responsible for rapid degradation of incretin hormones, plays a pivotal role in blood glucose regulation, and its inhibition serves as an effective strategy maintaining homeostasis. The aim this study was to investigate effect enzymatic hydrolysis on structure buffalo casein DPP-4 inhibitory activity. Results demonstrated that Flavorzyme effectively hydrolyzed casein, evidenced by scanning electron microscopy electrophoretic analysis, with degree reaching maximum value (20.05 ± 0.14%) after 3 h. results circular dichroism spectra, well endogenous exogenous fluorescence indicated significant alterations secondary tertiary structures following hydrolysis. Additionally, found increase longer times. hydrolysate obtained h highest level inhibition, half-maximal concentration (IC

Язык: Английский

Процитировано

1
Research Progress on the Clinical Application of GLP-1RA Drug Semaglutide DOI

曦 陈

Advances in Clinical Medicine, Год журнала: 2024, Номер 14(08), С. 853 - 861

Опубликована: Янв. 1, 2024

Язык: Английский

Процитировано

0
Heme Oxygenase-1 as Crucial Biomarker for Detecting Oxidative Stress and Some Parameters in a Sample of Obese Patients with and without Diabetes DOI Creative Commons

Mays Mohammed Abdullah,

Fayhaa Muqdad Khaleel

Clinical Diabetology, Год журнала: 2024, Номер 13(6), С. 349 - 357

Опубликована: Дек. 20, 2024

Objective: Obesity is a significant contributor to various metabolic disorders, including type 2 diabetes (T2D), resulting in heightened oxidative stress. This study aims examine the levels of heme oxygenase-1 (HO-1), their correlation with high-density lipoprotein (HDL) concentration, and influence on onset T2D obese individuals diabetes. Materials methods: The comprised 150 samples categorized into 3 groups, each group further subdivided subgroups: males females aged 30 65 years. Samples were collected at AL-Kindy Teaching Hospital. All sample variables for fasting subjects every assessed. colorimetric approach was employed biochemical assays, encompassing blood sugar (FBS) lipid profiles. Insulin, HO-1, dipeptidyl peptidase-4 (DPP-4) also quantified using ELISA. Subsequently, we statistical analysis elucidate results. Results: Compared obesity group, HO-1 HDL concentrations higher [(18.0 ± 0.40), (39.86 1.26) vs. (10.41 0.74), (36.27 0.85), (ng/mL), (mg/dL), respectively]. showed insulin resistance compared control groups [(4.19 0.874) (1.21 0.39), (0.74 0.142)]. male had elevated result that applied females. Conclusions: enzyme than group. We found positive association between increased may serve as biomarker predict development or other diseases.

Язык: Английский

Процитировано

0