Abstract
Muscular
atrophy
accompanied
by
neuromuscular
junction
(NMJ)
denervation
is
often
observed
after
long-term
chronic
diseases
and
aging
associated
with
substantial
morbidity
mortality.
Here,
we
report
that
histone
methyltransferase
G9a
elevated
in
the
muscle
of
muscular
model
mice
muscle-specific
deficiency
(
Ehmt2
Ckmm−
KO)
alleviates
both
aged
denervated
mice.
Moreover,
increased
nerve-to-myofiber
ratios
Agrin-Lrp4-MuSK
signaling,
which
maintains
NMJ,
are
found
KO
Together,
these
data
suggest
promotes
disrupts
NMJ;
thus,
inhibiting
level
may
be
a
potential
therapy
for
atrophy.
Frontiers in Cell and Developmental Biology,
Год журнала:
2025,
Номер
13
Опубликована: Май 2, 2025
Introduction
Peripheral
nerve
injuries
remain
a
significant
clinical
challenge,
particularly
in
severe
neurotmesis
requiring
complex
therapeutic
interventions
to
restore
functionality.
This
study
aimed
evaluate
the
pro-regenerative
potential
of
combining
neural
guide
conduits
with
conditioned
medium
from
olfactory
mucosa
mesenchymal
stem
cells,
compared
gold-standard
surgical
techniques.
Methods
The
was
conducted
using
validated
ovine
model
common
peroneal
injury.
Recovery
assessed
over
24
weeks
through
functional,
kinematic,
ultrasonographic,
and
electrophysiological
evaluations,
complemented
by
post-mortem
stereology
muscle
histomorphometry.
Results
All
approaches
promoted
regeneration,
resulting
notable
functional
structural
improvements.
However,
irregularities
were
observed,
as
did
not
consistently
outperform
standard
Additionally,
recovery
often
fell
short
normal
values
control
group.
Discussion
These
findings
highlight
complexity
peripheral
regeneration
challenging
scenarios
underscore
translational
biomaterials
cell
medium-based
therapies.
observed
emphasize
need
for
further
research
animal
models
before
application
real
cases.
Such
studies
are
essential
refine
strategies,
address
inconsistencies,
establish
viable
tool
repair.
Metabolites,
Год журнала:
2025,
Номер
15(5), С. 316 - 316
Опубликована: Май 8, 2025
Background:
Maillard
reaction
products
(MRPs)
are
known
for
their
antioxidant
properties;
however,
effects
on
muscle
cells
remain
unclear.
This
study
aims
to
elucidate
the
of
MRPs
hypertrophy
and
atrophy
in
C2C12
myotubes.
Methods:
were
prepared
by
heating
L-lysine
D-glucose,
activity
was
assessed
using
2,2-diphenyl-1-picrylhydrazyl
(DPPH)
radical
scavenging
assay.
Subsequently,
mouse
myoblasts
cultured
with
until
myotubes
formed,
diameters
measured
assess
hypertrophic
atrophic
changes.
Akt
phosphorylation
evaluated
Western
blotting,
gene
expression
levels
analyzed
via
quantitative
PCR.
Results:
The
exhibited
high
DPPH
MRP
treatment
significantly
increased
average
myotube
diameter
approximately
40%
enlarged
largest
up
80%,
potentially
mediated
enhanced
phosphorylation.
Under
dexamethasone-induced
atrophy,
modestly
attenuated
reduction
20%,
although
effect
not
statistically
significant,
did
alter
fusion
index
either.
Quantitative
PCR
analysis
revealed
that
reduced
mRNA
Nfe2l2,
a
key
regulator
response,
whereas
it
had
no
notable
genes
related
myoblast
proliferation
(Myod1),
differentiation
(Myog),
(Igf1),
(Foxo1
Trim63),
oxidative
stress
(Cat,
Gclc,
Nqo1).
Conclusions:
Our
findings
suggested
possess
promote
signaling.
highlighted
potential
as
functional
ingredients
promoting
health,
though
further
vivo
studies
required
validate
physiological
relevance.
Frontiers in Neurology,
Год журнала:
2025,
Номер
16
Опубликована: Май 13, 2025
Previous
research
has
highlighted
the
pivotal
role
of
angiogenesis
in
facilitating
nerve
function
repair
following
injury.
In
this
study,
we
employed
polydopamine
(PDA)
to
modify
polycaprolactone
(PCL)
and
subsequently
loaded
it
with
roxadustat
(ROX),
thereby
constructing
a
vascularized
conduit
for
10
mm
sciatic
defect
rats.
At
2
weeks
post-surgery,
new
blood
vessels
were
evaluated
by
immunofluorescence
staining.
Twelve
comprehensive
suite
assessments
was
conducted
evaluate
efficacy
conduit,
including
gait
analysis,
determination
gastrocnemius
muscle
wet
weight
recovery,
electrophysiological
examination
compound
action
potential
(CMAP),
Masson
staining
fiber
cross-sectional
area,
toluidine
blue
assess
total
number
regenerated
myelinated
fibers,
electron
microscopic
observation
myelin
sheath
thickness.
Our
findings
revealed
that
ROX@PDA@PCL
could
promote
proliferation
vascular
endothelial
cells
significantly
enhance
nerves
(
p
<
0.05).
Regarding
recovery
neurological
function,
compared
PDA@PCL
PCL
groups,
group
exhibited
superior
outcomes
functional
index
(SFI),
CMAP,
ratio,
These
indices
approached
those
autologous
group,
but
still
lower
than
autograft
The
study
underscores
graft
(ROX@PDA@PCL),
constructed
through
PDA-mediated
loading
ROX
onto
PCL,
recovery.
present
promising
therapeutic
approach
clinical
peripheral
defects.
Journal of Translational Medicine,
Год журнала:
2024,
Номер
22(1)
Опубликована: Ноя. 2, 2024
Muscle
atrophy
caused
by
denervation
is
common
in
neuromuscular
diseases,
leading
to
loss
of
muscle
mass
and
function.
However,
a
comprehensive
understanding
the
overall
molecular
network
changes
during
still
deficient,
hindering
development
effective
treatments.
Journal of Nanobiotechnology,
Год журнала:
2024,
Номер
22(1)
Опубликована: Дек. 19, 2024
Numerous
conduits
have
been
developed
to
improve
peripheral
nerve
regeneration.
However,
challenges
remain,
including
remote
control
of
conduit
function,
and
programmed
cell
behaviors
like
orientation.
We
synthesized
Fe3O4-MnO2@Zirconium-based
Metal-organic
frameworks@Retinoic
acid
(FMZMR)
core-shell
assessed
their
impact
on
Schwann
function
behavior
within
made
from
decellularized
human
umbilical
arteries
(DHUCA)
under
magnetic
field
(MF).
FMZMR
core-shell,
featuring
a
spherical
porous
structure
catalytic
properties,
effectively
scavenges
radicals
facilitates
controlled
drug
release
MF.
The
histology
the
DHUCA
indicates
effective
decellularization
with
adequate
tensile
strength
Young's
modulus
for
sciatic
In-vitro
results
demonstrate
that
is
biocompatible
promotes
proliferation
through
remotely
release.
Furthermore,
its
synergy
MF
enhances
orientation
increases
neurite
length
by
~
1.93-fold.
Functional
histological
evaluations
indicate
combined
regeneration,
decreases
muscle
atrophy,
new
neuron
growth
myelin
formation,
without
negatively
affecting
vital
tissues.
This
study
suggests
synergistic
effect
can
alleviate
some
treatment
challenges.
Journal of Orthopaedic Surgery and Research,
Год журнала:
2024,
Номер
19(1)
Опубликована: Дек. 31, 2024
Tuina
is
an
effective
treatment
for
the
decrease
of
skeletal
muscle
atrophy
after
peripheral
nerve
injury.
However,
underlying
mechanism
action
remains
unclear.
This
study
aimed
to
explore
mechanisms
tuina
in
rats
with
sciatic
injury
(SNI).
We
established
SNI
rat
model.
After
intervention,
curative
effects
were
evaluated
by
behavioral
assessment,
function
index,
and
index
(MAI).
Pathological
changes
observed
transmission
electron
microscopy
immunofluorescence.
Insulin-like
growth
factor
1
(IGF-1),
forkhead
box
O
(FoxO)
p-FoxO
levels
detected
using
enzyme-linked
immunosorbent
assay.
Western
blotting
was
performed
detect
expression
proteins
involved
PI3K/AKT
signaling
pathway.
Behavioral
MAI
revealed
that
had
significantly
improved
compared
model
group.
Transmission
showed
ultramicrostructure.
CD31
immunofluorescence
microcirculation.
Furthermore,
we
differentially
regulated
IGF-1,
FoxO
p-FoxO,
protein
p-Phosphoinositide
3-kinase
(p-PI3K),
p-AKT,
vascular
endothelial
anterior
tibial
soleus
muscles.
could
effectively
inhibit
via
microcirculation
pathway
a
regulating
IGF-1
FoxO.
The
may
involve
PI3K/Akt
Research Square (Research Square),
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 28, 2024
Abstract
Background
Tuina
has
been
shown
to
be
an
effective
treatment
for
the
decrease
of
skeletal
muscle
atrophy
after
Peripheral
nerve
injury
(PNI).
However,
its
mechanism
is
unclear.
This
study
aimed
explore
underlying
mechanisms
tuina
on
rats
with
sciatic
(SNI).
Methods
A
rat
model
SNI
was
established.
After
a
total
20
times
intervention,
curative
effects
were
evaluated
by
behavioral
assessment,
function
index
and
(MAI).
The
pathological
changes
observed
transmission
electron
microscopy
immunofluorescence.
levels
IGF-1
FoxO
detected
enzyme-linked
immunosorbent
assay
(ELISA).
Western
blotting
used
detect
expression
proteins
in
PI3K/AKT
signaling
pathway.
Result
MAI
proved
that
significantly
improved
compared
group.
Transmission
showed
ultramicrostructure
immunofluorescence
CD31
microcirculation.
Further,
we
differentially
regulated
protein
p-PI3K,
p-AKT
VEGF
anterior
tibial
soleus
muscle.
Conclusion
could
effectively
inhibit
via
microcirculation
pathway
SNI,
regulating
IGF-1,
FoxO.
action
might
PI3K/Akt
