G9a promotes muscular atrophy in chronic aging and acute denervation DOI Creative Commons
Ying Jin, Wei Kang, Xiaolong Ji

et al.

Animal Diseases, Journal Year: 2024, Volume and Issue: 4(1)

Published: Nov. 5, 2024

Abstract Muscular atrophy accompanied by neuromuscular junction (NMJ) denervation is often observed after long-term chronic diseases and aging associated with substantial morbidity mortality. Here, we report that histone methyltransferase G9a elevated in the muscle of muscular model mice muscle-specific deficiency ( Ehmt2 Ckmm− KO) alleviates both aged denervated mice. Moreover, increased nerve-to-myofiber ratios Agrin-Lrp4-MuSK signaling, which maintains NMJ, are found KO Together, these data suggest promotes disrupts NMJ; thus, inhibiting level may be a potential therapy for atrophy.

Language: Английский

Microhook Nerve Connector-Assisted Polyethylene Glycol Fusion for Immediate Restoration of Axon Continuity in a Rodent Model DOI
Geetanjali Bendale,

Andrew Azdell,

Jonathan E. Miller

et al.

The Journal Of Hand Surgery, Journal Year: 2025, Volume and Issue: unknown

Published: April 1, 2025

Language: Английский

Citations

0
Salvianolic Acid A Alleviates Sciatic Nerve Injury in Rats Via Inhibiting NLRP3 Inflammasome Through the Activation of Schwann Cells Autophagy DOI

Mingfu Fu,

Fenghua Zhang, Yanli Song

et al.

Neurochemical Research, Journal Year: 2025, Volume and Issue: 50(3)

Published: April 25, 2025

Language: Английский

Citations

0
Combined use of olfactory mucosal mesenchymal stem cells conditioned medium and neural guide conduits promotes nerve regeneration in an ovine model DOI Creative Commons
Rui Alvites, Bruna Lopes, André Coelho

et al.

Frontiers in Cell and Developmental Biology, Journal Year: 2025, Volume and Issue: 13

Published: May 2, 2025

Introduction Peripheral nerve injuries remain a significant clinical challenge, particularly in severe neurotmesis requiring complex therapeutic interventions to restore functionality. This study aimed evaluate the pro-regenerative potential of combining neural guide conduits with conditioned medium from olfactory mucosa mesenchymal stem cells, compared gold-standard surgical techniques. Methods The was conducted using validated ovine model common peroneal injury. Recovery assessed over 24 weeks through functional, kinematic, ultrasonographic, and electrophysiological evaluations, complemented by post-mortem stereology muscle histomorphometry. Results All approaches promoted regeneration, resulting notable functional structural improvements. However, irregularities were observed, as did not consistently outperform standard Additionally, recovery often fell short normal values control group. Discussion These findings highlight complexity peripheral regeneration challenging scenarios underscore translational biomaterials cell medium-based therapies. observed emphasize need for further research animal models before application real cases. Such studies are essential refine strategies, address inconsistencies, establish viable tool repair.

Language: Английский

Citations

0
Therapeutic potential of omaveloxolone in counteracting muscle atrophy post-denervation: a multi-omics approach DOI Creative Commons

Sulong Wang,

Xin Yang, Kai Liu

et al.

Journal of Translational Medicine, Journal Year: 2024, Volume and Issue: 22(1)

Published: Nov. 2, 2024

Muscle atrophy caused by denervation is common in neuromuscular diseases, leading to loss of muscle mass and function. However, a comprehensive understanding the overall molecular network changes during still deficient, hindering development effective treatments.

Language: Английский

Citations

1
Synergic effects of core-shell nanospheres and magnetic field for sciatic nerve regeneration in decellularized artery conduits with Schwann cells DOI Creative Commons
Majid Sharifi, Majid Salehi,

Somayeh Ebrahimi‐Barough

et al.

Journal of Nanobiotechnology, Journal Year: 2024, Volume and Issue: 22(1)

Published: Dec. 19, 2024

Numerous conduits have been developed to improve peripheral nerve regeneration. However, challenges remain, including remote control of conduit function, and programmed cell behaviors like orientation. We synthesized Fe3O4-MnO2@Zirconium-based Metal-organic frameworks@Retinoic acid (FMZMR) core-shell assessed their impact on Schwann function behavior within made from decellularized human umbilical arteries (DHUCA) under magnetic field (MF). FMZMR core-shell, featuring a spherical porous structure catalytic properties, effectively scavenges radicals facilitates controlled drug release MF. The histology the DHUCA indicates effective decellularization with adequate tensile strength Young's modulus for sciatic In-vitro results demonstrate that is biocompatible promotes proliferation through remotely release. Furthermore, its synergy MF enhances orientation increases neurite length by ~ 1.93-fold. Functional histological evaluations indicate combined regeneration, decreases muscle atrophy, new neuron growth myelin formation, without negatively affecting vital tissues. This study suggests synergistic effect can alleviate some treatment challenges.

Language: Английский

Citations

1
Tuina alleviates the muscle atrophy induced by sciatic nerve injury in rats through regulation of PI3K/Akt signaling DOI Creative Commons
Yingqi Zhang, Hanyu Zhang, Jiayue Liu

et al.

Journal of Orthopaedic Surgery and Research, Journal Year: 2024, Volume and Issue: 19(1)

Published: Dec. 31, 2024

Tuina is an effective treatment for the decrease of skeletal muscle atrophy after peripheral nerve injury. However, underlying mechanism action remains unclear. This study aimed to explore mechanisms tuina in rats with sciatic injury (SNI). We established SNI rat model. After intervention, curative effects were evaluated by behavioral assessment, function index, and index (MAI). Pathological changes observed transmission electron microscopy immunofluorescence. Insulin-like growth factor 1 (IGF-1), forkhead box O (FoxO) p-FoxO levels detected using enzyme-linked immunosorbent assay. Western blotting was performed detect expression proteins involved PI3K/AKT signaling pathway. Behavioral MAI revealed that had significantly improved compared model group. Transmission showed ultramicrostructure. CD31 immunofluorescence microcirculation. Furthermore, we differentially regulated IGF-1, FoxO p-FoxO, protein p-Phosphoinositide 3-kinase (p-PI3K), p-AKT, vascular endothelial anterior tibial soleus muscles. could effectively inhibit via microcirculation pathway a regulating IGF-1 FoxO. The may involve PI3K/Akt

Language: Английский

Citations

1
Tuina alleviates the muscle atrophy of sciatic nerve injury rats through regulating PI3K/Akt signaling DOI Creative Commons

英奇 张,

羽羽 张,

嘉悦 刘

et al.

Research Square (Research Square), Journal Year: 2024, Volume and Issue: unknown

Published: Aug. 28, 2024

Abstract Background Tuina has been shown to be an effective treatment for the decrease of skeletal muscle atrophy after Peripheral nerve injury (PNI). However, its mechanism is unclear. This study aimed explore underlying mechanisms tuina on rats with sciatic (SNI). Methods A rat model SNI was established. After a total 20 times intervention, curative effects were evaluated by behavioral assessment, function index and (MAI). The pathological changes observed transmission electron microscopy immunofluorescence. levels IGF-1 FoxO detected enzyme-linked immunosorbent assay (ELISA). Western blotting used detect expression proteins in PI3K/AKT signaling pathway. Result MAI proved that significantly improved compared group. Transmission showed ultramicrostructure immunofluorescence CD31 microcirculation. Further, we differentially regulated protein p-PI3K, p-AKT VEGF anterior tibial soleus muscle. Conclusion could effectively inhibit via microcirculation pathway SNI, regulating IGF-1, FoxO. action might PI3K/Akt

Language: Английский

Citations

0
G9a promotes muscular atrophy in chronic aging and acute denervation DOI Creative Commons
Ying Jin, Wei Kang, Xiaolong Ji

et al.

Animal Diseases, Journal Year: 2024, Volume and Issue: 4(1)

Published: Nov. 5, 2024

Abstract Muscular atrophy accompanied by neuromuscular junction (NMJ) denervation is often observed after long-term chronic diseases and aging associated with substantial morbidity mortality. Here, we report that histone methyltransferase G9a elevated in the muscle of muscular model mice muscle-specific deficiency ( Ehmt2 Ckmm− KO) alleviates both aged denervated mice. Moreover, increased nerve-to-myofiber ratios Agrin-Lrp4-MuSK signaling, which maintains NMJ, are found KO Together, these data suggest promotes disrupts NMJ; thus, inhibiting level may be a potential therapy for atrophy.

Language: Английский

Citations

0