Biosynthesis of lysosomally escaped apoptotic bodies inhibits inflammasome synthesis in macrophages DOI Creative Commons
Jiayi Mao,

Wenzheng Xia,

Yanglin Wu

и другие.

Research, Год журнала: 2024, Номер 8

Опубликована: Дек. 27, 2024

Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3 (NLRP3) macrophages, resulting extensive inflammatory infiltration impaired wound healing. Targeted suppression of the NLRP3 inflammasome shows promise reducing macrophage disruptions. However, challenges such as drug off-target effects degradation via lysosomal capture remain during treatment. In this study, engineered apoptotic bodies (BHB-dABs) derived from adipose stem cells loaded with β-hydroxybutyric acid (BHB) were synthesized biosynthesis. These vesicles target M1-type which highly express folic microenvironment, facilitate escape through 1,2-distearoyl-sn-propyltriyl-3-phosphatidylethanolamine-polyethylene glycol functionalization, may enhance efficacy inhibition for managing wounds. vitro studies demonstrated biocompatibility BHB-dABs, their selective targeting ability to release BHB within microenvironment signaling. nanovesicles exhibited escape, anti-inflammatory, mitochondrial protection, endothelial cell vascularization properties. vivo experiments that BHB-dABs recovery inflammation angiogenesis, accelerating functionalized efficiently deliver inhibitors using a dual strategy macrophages promoting escape. This approach represents novel therapeutic effectively treating chronic

Язык: Английский

Intelligent bacteria‐targeting ZIF‐8 composite for fluorescence imaging‐guided photodynamic therapy of drug‐resistant superbug infections and burn wound healing DOI Creative Commons

Xiaoxue Li,

Wei Wang,

Qiuxia Gao

и другие.

Exploration, Год журнала: 2024, Номер 4(6)

Опубликована: Апрель 19, 2024

Abstract Infected burn wounds are characterized by persistent drug‐resistant bacterial infection coupled with an inflammatory response, impeding the wound‐healing process. In this study, intelligent nanoparticle system (CCM+TTD@ZIF‐8 NPs) was prepared using curcumin (CCM), aggregation‐induced emission luminogens (TTD), and ZIF‐8 for infection‐induced wound healing. The CCM+TTD@ZIF‐8 NPs showed multiple functions, including bacteria targeting, fluorescence imaging pH response‐guided photodynamic therapy (PDT), anti‐inflammatory. positive charges of allowed targeting in infected wounds, thereby realizing emitting red at site upon blue light irradiation. pH‐responsive characteristics also enabled controllable CCM release onto site, promoting specific accumulation ROS outstanding bactericidal efficacy against Staphylococcus aureus (S. aureus) Pseudomonas aeruginosa (P. aeruginosa) strains vitro/in vivo. Additionally, due to excellent effect anti‐inflammatory properties combined irradiation, regeneration epidermal tissue, angiogenesis, collagen deposition achieved, accelerating healing process wounds. Therefore, multifunctional provides great potential

Язык: Английский

Процитировано

28

Antibiotic-loaded lactoferrin nanoparticles as a platform for enhanced infection therapy through targeted elimination of intracellular bacteria DOI Creative Commons
Wei Wang,

Wanying Mo,

Xue Xiao

и другие.

Asian Journal of Pharmaceutical Sciences, Год журнала: 2024, Номер 19(4), С. 100926 - 100926

Опубликована: Май 6, 2024

Intracellular bacteria can multiply inside host cells and manipulate their biology, the efficacy of traditional antibiotic drug therapy for intracellular is limited by inadequate accumulation. Fighting against these stealthy has been a long-standing challenge. Here, system stimuli-responsive lactoferrin (Lf) nanoparticles prepared using protein self-assembly technology to deliver broad-spectrum rifampicin (Rif) (Rif@Lf NPs) enhanced infection through targeted elimination bacteria. Compared Rif@BSA NPs, Rif@Lf NPs specifically target macrophages infected bacteria, thus increasing accumulation Rif within macrophages. Subsequently, with positive surface charge further displayed adherence released rapidly in redox-responsive manner. Combined antibacterial activities Lf Rif, showed abilities biofilms. As result, high safety exhibited excellent therapeutic disease models subcutaneous infection, sepsis, bacterial keratitis. Taken together, antibiotic-loaded present promising platform combat pathogen infections

Язык: Английский

Процитировано

10

Uptake of Biomimetic Nanovesicles by Granuloma for Photodynamic Therapy of Tuberculosis DOI Creative Commons

Huanhuan Wang,

Xiaoxue Li, Peiran Li

и другие.

ACS Omega, Год журнала: 2025, Номер 10(7), С. 6679 - 6688

Опубликована: Фев. 11, 2025

The antimicrobial resistance of Mycobacterium tuberculosis (M. tuberculosis) is a challenge in the antibiotic treatment (TB). Herein, we aimed to examine photodynamic therapy for TB that has low risk drug and involves biomimetic macrophage membranes combined with photosensitizer, chlorin e6 (Ce6; hereinafter, C-MV). We used marinum marinum), waterborne pathogen closely related M. tuberculosis, which causes TB-like infections ectotherms but not humans. mouse tail granuloma model induced by relatively mature developed our team. C-MV nanoparticles were prepared injected intravenously, showing longevity circulation due properties membrane, protects them from being eliminated blood. They then guided tuberculous granulomas, helping deliver precise therapy. Ce6 classical photosensitizer triggers production reactive oxygen species under laser irradiation, causing death. showed good compatibility long time, effectively inhibiting proliferation infiltration marinum, providing new paradigm treatment.

Язык: Английский

Процитировано

1

Extracellular Vesicle‐Inspired Therapeutic Strategies for the COVID‐19 DOI
Ziwei Hu, Wei Wang,

Ying Lin

и другие.

Advanced Healthcare Materials, Год журнала: 2024, Номер 13(29)

Опубликована: Июнь 25, 2024

Emerging infectious diseases like coronavirus pneumonia (COVID-19) present significant challenges to global health, extensively affecting both human society and the economy. Extracellular vesicles (EVs) have demonstrated remarkable potential as crucial biomedical tools for COVID-19 diagnosis treatment. However, due limitations in performance titer of natural vesicles, their clinical use remains limited. Nonetheless, EV-inspired strategies are gaining increasing attention. Notably, biomimetic inspired by EVs, possess specific receptors that can act "Trojan horses," preventing virus from infecting host cells. Genetic engineering enhance these enabling them carry more receptors, significantly specificity absorbing novel coronavirus. Additionally, inherit numerous cytokine parent cells, allowing effectively mitigate "cytokine storm" adsorbing pro-inflammatory cytokines. Overall, this strategy offers new avenues treatment emerging diseases. Herein, review systematically summarizes current applications COVID-19. The status associated with implementation also discussed. goal is provide insights into design expand application combating

Язык: Английский

Процитировано

7

A Mucous Permeable Local Delivery Strategy Based on Manganese-Enhanced Bacterial Cuproptosis-like Death for Bacterial Pneumonia Treatment DOI
Shiyuan Hua,

Huiqun Hu,

Jin Liu

и другие.

ACS Nano, Год журнала: 2024, Номер 18(46), С. 31923 - 31940

Опубликована: Ноя. 7, 2024

Bacterial pneumonia is one of the most challenging global infectious diseases with high morbidity and mortality. Considering antibiotic abuse resistance bacterial biofilms, a variety metal-based materials have been developed. However, due to oxygen environment lungs, some aerobic infection bacteria tolerance ROS, based on ROS may not achieve good therapeutic effects. Inspired by sensitivity cuproptosis respiratory cells, we designed copper composite antibacterial nanoparticle found that it can effectively induce cuproptosis-like death in lungs. To address challenge

Язык: Английский

Процитировано

7

Silver nanoparticle-loaded Konjac Glucomannan/silk fibroin composite hydrogels for enhanced wound healing DOI
Yanjun Li,

Donglan Yong,

Jingwen Shen

и другие.

International Journal of Biological Macromolecules, Год журнала: 2025, Номер unknown, С. 140199 - 140199

Опубликована: Янв. 1, 2025

Язык: Английский

Процитировано

0

Pioneering Topical Ointment Intervention for Unprecedented Antimicrobial and Diabetic Wound Management with Phenylpropanoids and Nano-Silver DOI

Hari Prasath Nagaiah,

Priya Dharsini Periyakaruppan Murugesan,

Chaughule Vrushali Ravindra Rupali

и другие.

The AAPS Journal, Год журнала: 2024, Номер 26(4)

Опубликована: Июнь 11, 2024

Язык: Английский

Процитировано

2

Synergistic Biofilter Tube for Promoting Scarless Tendon Regeneration DOI Creative Commons
Renhao Yang, Yidong Xu,

Renxuan Li

и другие.

Nano Letters, Год журнала: 2024, Номер 24(24), С. 7381 - 7388

Опубликована: Июнь 4, 2024

Inspired by the imbalance between extrinsic and intrinsic tendon healing, this study fabricated a new biofilter scaffold with hierarchical structure based on melt electrowriting technique. The outer multilayered fibrous connected porous characteristics provides novel passageway for vascularization isolates penetration of scar fibers, which can be referred to as process. In vitro experiments found that architecture in layer effectively prevent cell infiltration, whereas aligned fibers inner promote recruitment growth, well expression tendon-associated proteins simulated friction condition. It was shown vivo process could healing reduce invasion. Herein, strategy indicates great potential design biomaterials balancing realizing scarless healing.

Язык: Английский

Процитировано

1

Biosynthesis of lysosomally escaped apoptotic bodies inhibits inflammasome synthesis in macrophages DOI Creative Commons
Jiayi Mao,

Wenzheng Xia,

Yanglin Wu

и другие.

Research, Год журнала: 2024, Номер 8

Опубликована: Дек. 27, 2024

Hyperglycemia and bacterial colonization in diabetic wounds aberrantly activate Nod-like receptor protein 3 (NLRP3) macrophages, resulting extensive inflammatory infiltration impaired wound healing. Targeted suppression of the NLRP3 inflammasome shows promise reducing macrophage disruptions. However, challenges such as drug off-target effects degradation via lysosomal capture remain during treatment. In this study, engineered apoptotic bodies (BHB-dABs) derived from adipose stem cells loaded with β-hydroxybutyric acid (BHB) were synthesized biosynthesis. These vesicles target M1-type which highly express folic microenvironment, facilitate escape through 1,2-distearoyl-sn-propyltriyl-3-phosphatidylethanolamine-polyethylene glycol functionalization, may enhance efficacy inhibition for managing wounds. vitro studies demonstrated biocompatibility BHB-dABs, their selective targeting ability to release BHB within microenvironment signaling. nanovesicles exhibited escape, anti-inflammatory, mitochondrial protection, endothelial cell vascularization properties. vivo experiments that BHB-dABs recovery inflammation angiogenesis, accelerating functionalized efficiently deliver inhibitors using a dual strategy macrophages promoting escape. This approach represents novel therapeutic effectively treating chronic

Язык: Английский

Процитировано

0