The major roles of intestinal microbiota and TRAF6/NF-κB signaling pathway in acute intestinal inflammation in mice, and the improvement effect by Hippophae rhamnoides polysaccharide

International Journal of Biological Macromolecules, Год журнала: 2025, Номер 296, С. 139710 - 139710

Опубликована: Янв. 9, 2025

Язык: Английский

---

Analysis revealed the molecular mechanism of oxidative stress-autophagy-induced liver injury caused by high alkalinity: integrated whole hepatic transcriptome and metabolome

Frontiers in Immunology, Год журнала: 2024, Номер 15

Опубликована: Июль 8, 2024

Introduction High-alkalinity water is a serious health hazard for fish and can cause oxidative stress metabolic dysregulation in livers. However, the molecular mechanism of liver damage caused by high alkalinity unclear. Methods In this study, 180 carp were randomly divided into control (C) group high-alkalinity (A25) cultured 56 days. High-alkalinity-induced injury was analysed using histopathological, whole-transcriptome, metabolomic analyses. Results Many autophagic bodies abundant mitochondrial membrane observed A25 group. High decreased superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GSH-Px) activity total antioxidant capacity (T-AOC) increased malondialdehyde (MDA) content tissues, causing liver. Transcriptome analysis revealed 61 differentially expressed microRNAs (miRNAs) 4008 mRNAs. Kyoto Encyclopedia Genes Genomes (KEGG) enrichment that mammalian target rapamycin (mTOR), forkhead box O (FoxO), mitogen-activated protein kinase (MAPK), autophagy signalling pathway mechanisms involved. causes results Bioinformatic predictions indicated Unc-51 Like Kinase 2 (ULK2) potential gene miR-140-5p, demonstrating triggered through miR-140-5p–ULK2 axis. Metabolomic concentrations cortisol 21-sulfate beta-aminopropionitrile significantly increased, while those creatine uracil decreased. Discussion The effects on whole-transcriptome miRNA-mRNA networks metabolomics approaches. Our study provides new insights highly alkaline water.

Язык: Английский

---

Astaxanthin ameliorates high-carbohydrate diet-induced ER stress, immunosuppression and hepatic glucose metabolism through AMPK/autophagy pathway in Channa argus

Aquaculture, Год журнала: 2024, Номер 598, С. 742010 - 742010

Опубликована: Дек. 13, 2024

Язык: Английский

---

Effects of taurine on the growth performance, diarrhea, oxidative stress and intestinal barrier function of weanling piglets

Frontiers in Veterinary Science, Год журнала: 2024, Номер 11

Опубликована: Авг. 7, 2024

Oxidative damage resulting from weaning stress significantly impacts the growth performance and health status of piglets. Taurine, a dietary antioxidant with diverse functions, was investigated in this study for its protective role against stress-induced oxidative underlying mechanism. Forty 28-day-old male castrated weaned piglets were randomly assigned to four groups. The control group received basal diet, while experimental groups fed diet supplemented 0.1, 0.2%, or 0.3% taurine over 28-day period.

Язык: Английский

---

Astaxanthin activated the SLC7A11/GPX4 pathway to inhibit ferroptosis and enhance autophagy, ameliorating dry eye disease

Frontiers in Pharmacology, Год журнала: 2024, Номер 15

Опубликована: Авг. 19, 2024

Dry eye disease (DED) is a common in clinical practice. The crucial pathogenesis of DED that hyperosmolarity activates oxidative stress signaling pathways corneal epithelial and immune cells and, thus, produces inflammatory molecules. complex pathological changes the dry still need to be elucidated facilitate treatment. In this study, we found astaxanthin (AST) can protect against through SLC7A11/GPX4 pathway. After treatment with AST, pathway was positively activated both vivo vitro , accompanied by enhanced autophagy decreased ferroptosis. hyperosmolarity-induced cells, AST increased expression ferritin promote iron storage reduce Fe 2+ overload. It glutathione (GSH) GPX4, scavenged reactive oxygen species (ROS) lipid peroxide, rescued mitochondrial structure prevent Furthermore, inhibition ferroptosis ferrostatin-1 (Fer-1), chelator deferoxamine mesylate (DFO), or could activate healthy autophagic flux. addition, mouse model, upregulated SLC7A11 GPX4 inhibited To summarize, ameliorate reinforcing pathway, which mainly affects stress, autophagy, processes.

Язык: Английский

---

Selenium toxicity in fishes: A current perspective

Chemosphere, Год журнала: 2024, Номер 364, С. 143214 - 143214

Опубликована: Авг. 29, 2024

Anthropogenic activities have led to increased levels of contaminants that pose significant threats aquatic organisms, particularly fishes. One such contaminant is Selenium (Se), a metalloid which released by various industrial including mining and fossil fuel combustion. crucial for physiological functions, however it can bioaccumulate become toxic at elevated concentrations. Given fishes are key predators in ecosystems major protein source humans, Se accumulation raises considerable ecological food safety concerns. induces toxicity the cellular level disrupting balance between reactive oxygen species (ROS) production antioxidant capacity leading oxidative damage. Chronic exposure impairs wide range critical functions metabolism, growth reproduction. also potent teratogen types adverse developmental effects fishes, mainly due its maternal transfer eggs. Moreover, persist across generations. Furthermore, Se-induced stress brain driver neurotoxicity, leads impairment several ecologically important behaviours cognition memory social preference interactions, anxiety response. Our review provides an up-to-date in-depth analysis while identifying knowledge gaps need be addressed future research greater insights into impact ecosystems.

Язык: Английский

---

Protection of glutamine: The NF-κB/MLCK/MLC2 signaling pathway mediated by tight junction affects oxidative stress, inflammation and apoptosis in snakehead (Channa argus)

Fish & Shellfish Immunology, Год журнала: 2025, Номер 158, С. 110131 - 110131

Опубликована: Янв. 17, 2025

Язык: Английский

---

Chronic Cr(VI) exposure-induced biotoxicity involved in liver microbiota-gut axis disruption in Phoxinus lagowskii Dybowski based on multi-omics technologies

Environmental Pollution, Год журнала: 2025, Номер unknown, С. 125759 - 125759

Опубликована: Янв. 1, 2025

Язык: Английский

---

Condensed tannin from Caragana korshinskii extraction and protection effects on intestinal barrier function in mice

Frontiers in Veterinary Science, Год журнала: 2025, Номер 12

Опубликована: Фев. 3, 2025

Caragana korshinskii tannins (CKT) were extracted by response surface methodology and the protection effect of CKT on jejunal mucosal barrier function mice was investigated. Firstly, this work presents extraction, purification characterization CKT. The results show that extraction conditions as follows: temperature 52°C, time 95 min, liquid-solid ratio 20:1 acetone volume fraction 62%. yield 5.34%. has a typical polyphenol peak with molecular weight 8.662 kDa is composed epigallocatechin, catechin, epigallocatechin gallate, epicatechin, gallocatechin, epicatechin-3-o-gallate catechin gallate molar 1:8.88:2.65:1.55:1.92:0.49:0.14. Additionally, showed strong antioxidants capacity in vitro . Secondly, growth performance mouse jejunum examined. Totally, sixty KM randomly divided into six treatment groups ( n = 10) using single-factor completely randomized experimental design. intragastrically administered 0, 25, 50, 100, 200, 400 mg/kg BW aqueous solution once day. gavage set at 0.2 mL per 10 g body weight, daily for 21 days. significantly improved physiological state intestine. strengthened intestinal physical upregulating expression Occludin ZO-1 decreasing levels serum diamine oxidase (DAO) D-lactate (D-LA). Regarding biochemical barrier, could upregulate activity gene superoxide dismutase (SOD) glutathione peroxidase (GSH-Px) content malondialdehyde (MDA) tissues. Generally, may be used functional feed additive to regulate function, thereby enhancing health intestine host.

Язык: Английский

---

The effects of restraint stress and orthodontic tooth movements on the intestinal epithelial structure and metabolic function in rats

PLoS ONE, Год журнала: 2025, Номер 20(2), С. e0319779 - e0319779

Опубликована: Фев. 27, 2025

Chronic stress and orthodontic treatment have been revealed to trigger systemic responses in rats. This study aimed investigate the effects of restraint on intestinal epithelial structure, barrier function, flora, metabolism Twenty 8-week-old male Wistar rats were randomly divided into four groups: sham-stressed non-orthodontic (CC), (CO), stressed (SC), (SO). The intervention involved subjecting for 21 days, while consisted maxillary first molar traction from days 8 21. Histological immunohistochemical staining used observe structure function colon. flora metabolite alterations investigated by 16S rRNA high-throughput sequencing untargeted metabolomics sequencing. Colonic tissue disruption, mucus cells reduction, a decreased expression tight junction proteins observed CO, SC, SO groups. Lactobacillus spp. abundance was significantly lower CO group than CC group. Prevotella SC groups differential enrichment pathways between each inter-group comparison might all be related amino acid biosynthesis, protein digestion absorption, cofactor biosynthesis. Both may adversely affect colonic types metabolites also affected cumulatively.

Язык: Английский

---