Simple SARS-CoV-2 concentration methods for wastewater surveillance in low resource settings

The Science of The Total Environment, Год журнала: 2023, Номер 912, С. 168782 - 168782

Опубликована: Ноя. 23, 2023

Язык: Английский

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Comparison of adsorption-extraction (AE) workflows for improved measurements of viral and bacterial nucleic acid in untreated wastewater

The Science of The Total Environment, Год журнала: 2023, Номер 908, С. 167966 - 167966

Опубликована: Окт. 28, 2023

The lack of standardized methods and large differences in virus concentration extraction workflows have hampered severe acute respiratory syndrome (SARS-CoV-2) wastewater surveillance data reporting practices. Numerous studies shown that adsorption-extraction (AE) method holds promise, yet several uncertainties remain regarding the optimal AE workflow. Several procedural components may influence recovered concentrations target DNA/RNA, including membrane types, homogenization instruments, speed duration, lysis buffer. In this study, 42 different varied these were compared to determine by quantifying endogenous SARS-CoV-2, human adenovirus (HAdV 40/41) a bacterial marker gene fecal pollution (Bacteroides HF183). Our findings suggest certain selected workflow had significant impact on SARS-CoV-2 concentrations, whereas it minimal HF183 no effect HAdV 40/41 concentrations. When comparing individual workflow, such as type (MF-Millipore™ 0.45 μm MCE vs. Isopore™ 0.40 μm) instruments (Precellys 24 homogenizer Vortex-Genie®-2), we found they 40/41, This suggests at least some consumables equipment are interchangeable. Buffer PM1 + TRIzol based yielded higher than other workflows. without chloroform. Similarly, speeds (5000-10,000 rpm) led increased but 40/41. indicate minor enhancements can improve recovery viruses bacteria from wastewater, leading outcomes efforts.

Язык: Английский

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Building-level wastewater surveillance of SARS-CoV-2 is associated with transmission and variant trends in a university setting

Environmental Research, Год журнала: 2022, Номер 215, С. 114277 - 114277

Опубликована: Сен. 7, 2022

Язык: Английский

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Monitoring COVID-19 spread in selected Prague's schools based on the presence of SARS-CoV-2 RNA in wastewater

The Science of The Total Environment, Год журнала: 2023, Номер 871, С. 161935 - 161935

Опубликована: Янв. 30, 2023

Язык: Английский

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Actionable wastewater surveillance: application to a university residence hall during the transition between Delta and Omicron resurgences of COVID-19

Frontiers in Public Health, Год журнала: 2023, Номер 11

Опубликована: Май 17, 2023

Wastewater surveillance has gained traction during the COVID-19 pandemic as an effective and non-biased means to track community infection. While most relies on samples collected at municipal wastewater treatment plants, is more actionable when are “upstream” where mitigation of transmission tractable. This report describes results for SARS-CoV-2 residence halls a university campus aimed preventing outbreak escalation by mitigating spread. Another goal was estimate fecal shedding rates in non-clinical setting. Passive sampling devices were deployed sewer laterals originating from frequency twice weekly fall 2021 Delta variant concern continued circulate across North America. A positive detection part routine late November triggered daily monitoring further isolated signal single wing one hall. Detection within over period 3 consecutive days led coordinated rapid antigen testing campaign targeting hall occupants identification isolation infected individuals. With knowledge number individuals COVID-19, estimated range 3.70 log10 gc ‧ g feces −1 5.94 . These reinforce efficacy early indicator infection congregate living settings. Detections can trigger public health measures ranging enhanced communications targeted quarantine.

Язык: Английский

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Exploration on wastewater-based epidemiology of SARS-CoV-2: Mimic relative quantification with endogenous biomarkers as internal reference

Heliyon, Год журнала: 2023, Номер 9(5), С. e15705 - e15705

Опубликована: Апрель 23, 2023

Wastewater-based epidemiology has become a powerful surveillance tool for monitoring the pandemic of COVID-19. Although it is promising to quantitatively correlate SARS-CoV-2 RNA concentration in wastewater with incidence community infection, there still no consensus on whether viral nucleic acid sewage should be normalized against abundance endogenous biomarkers and which biomarker used as reference normalization. Here, several candidate normalization signal municipal were evaluated. The human fecal indicator virus (crAssphage) data both DNA viruses its intrinsic nature high stable content sewage. Without constructing standard curves, relative quantification can COVID-19 incidence, was proved via mimic experiments by spiking pseudovirus different concentrations samples. Dilution pseudovirus-seeded did not affect acid, demonstrating that overcome dilution effects caused greywater input, precipitation and/or groundwater infiltration. process concentration, recovery detection consistent RNA. Thus, necessary co-quantify because only an internal normalization, but also control.

Язык: Английский

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Evaluation of four human-associated fecal biomarkers in wastewater in Southern Ontario

The Science of The Total Environment, Год журнала: 2023, Номер 904, С. 166542 - 166542

Опубликована: Сен. 1, 2023

Язык: Английский

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Assessment of environmental factors influencing SARS-CoV-2 in Vietnam's surface water across two years of clinical data

The Science of The Total Environment, Год журнала: 2024, Номер 957, С. 177449 - 177449

Опубликована: Ноя. 19, 2024

Wastewater-based epidemiology (WBE) is an effective, non-invasive method for monitoring the spread of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) by tracking viral prevalence in water. This study aimed to investigate presence SARS-CoV-2 surface water Vietnam over two years. One-step quantitative reverse transcription polymerase chain reaction (qRT-PCR) assays were employed quantify and its variant-specific mutation sites (G339D/E484A) pepper mild mottle virus (PMMoV) from a total 315 samples (105 per type) compare with reported Coronavirus disease 2019 (COVID-19) cases environmental factors. was detected 38 % (40/105), 43 (45/105), 39 (41/105) Sites A, B, C, respectively, concentrations 3.0-5.6 log

Язык: Английский

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Evaluating Wastewater Quality Parameters as an Alternative or Complement to Molecular Indicators for Normalization during SARS-CoV-2 Wastewater-Based Epidemiology

Environments, Год журнала: 2024, Номер 11(4), С. 80 - 80

Опубликована: Апрель 12, 2024

Measuring fecal nucleic acid indicators for data normalization can increase costs during wastewater-based epidemiology (WBE). The efficacy of routinely assayed water quality parameters was assessed as an alternative or complement to viral indicator their utility in adjusting measured SARS-CoV-2 gene concentrations improve the relationship between wastewater molecular and clinical COVID-19 case data. This research covers two study designs: grab samples collected from sewers serving Ohio State University campus composite influent at five treatment plants (WWTPs) across state Ohio. Weekly mandatory testing used monitor infections student population, statewide cases were reported through voluntary testing. Statewide WWTP results showed significant strong correlation confirmed cases, increased when normalized by flow additionally pH, total suspended solids, temperature, but decreased a (PMMoV). On campus, correlations generally not unless PMMoV and/or UV absorbance parameters. Because are some may be autosamplers, incorporating WBE models could minimize cellular decrease costs.

Язык: Английский

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Evaluating various composite sampling modes for detecting pathogenic SARS-CoV-2 virus in raw sewage

Frontiers in Microbiology, Год журнала: 2023, Номер 14

Опубликована: Ноя. 23, 2023

Inadequate sampling approaches to wastewater analyses can introduce biases, leading inaccurate results such as false negatives and significant over- or underestimation of average daily viral concentrations, due the sporadic nature input. To address this challenge, we conducted a field trial within University Tennessee residence halls, employing different composite modes that encompassed time intervals (1 h, 2 4 6 24 h) across various windows (morning, afternoon, evening, late-night). Our primary objective was identify optimal approach for generating representative samples SARS-CoV-2 from raw wastewater. Utilizing reverse transcription-quantitative polymerase chain reaction, quantified levels RNA pepper mild mottle virus (PMMoV) in sewage. findings consistently demonstrated PMMoV RNA, an indicator human fecal contamination water environment, exhibited higher abundance lower variability compared pathogenic RNA. Significantly, both greater 1 h individual throughout entire period, contrasting with stability observed other time-based samples. Through comprehensive analysis using Quade Nonparametric ANCOVA test date, concentration site covariates, concluded sampler during focused morning window is pragmatic cost-effective strategy achieving single day wastewater-based epidemiology applications. This method has potential significantly enhance accuracy reliability data collected at community level, thereby contributing more informed public health decision-making pandemic.

Язык: Английский

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