bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2023,
Номер
unknown
Опубликована: Фев. 8, 2023
Abstract
Extreme
high
thermal
conditions
on
tropical
rocky
shores
are
challenging
to
the
survival
of
intertidal
ectotherms.
Yet,
many
species
highly
successful
in
these
environments
part
due
their
ability
regulate
intrinsic
mechanisms
associated
with
physiological
stress
and
metabolic
demand.
More
recently,
there
has
been
a
growing
awareness
that
other
extrinsic
mechanisms,
such
as
animal-associated
microbial
communities,
can
also
influence
tolerance
ectotherms
under
stressful
conditions.
However,
extent
which
functionally
linked
overall
adaptive
response
animals
temperature
change
is
poorly
understood.
Here,
we
examined
dynamics
potential
interactions
high-supratidal
oyster,
Isognomon
nucleus
.
We
found
oysters
modulate
internal
biochemistry
(oxidized
PUFA
products,
including
5-F
2t
-IsoP,
10-F
4t
-NeuroP,
13-F
16-F
1t
-PhytoP)
regulation
cope
during
periods
extreme
temperatures
when
emersed.
while
detected
variation
alpha
diversity
(ASV
richness
Shannon
index),
dominant
taxa
functions
across
time,
no
association
was
host
biochemical
profiles.
The
findings
here
suggest
condition
within
independently
responses
microbiome
Together,
may
contribute
zone.
One Health,
Год журнала:
2025,
Номер
20, С. 100973 - 100973
Опубликована: Янв. 13, 2025
Oyster
aquaculture
world-wide
is
a
booming
industry
that
can
provide
many
benefits
to
coastal
habitats,
including
economic,
ecosystem-level,
and
cultural
benefits.
Oysters
present
several
risks
for
human
consumption,
transmission
of
parasites,
bacterial
viral
pathogens.
microbiomes
are
well-defined,
but
their
connection
the
incidence
pathogens,
humans
or
others,
unclear.
Furthermore,
viruses
associated
with
oysters
largely
unknown,
humans,
animals,
ecosystem
health
has
not
been
explored.
Here,
we
employed
One
Health
framework
modern
molecular
techniques,
16S
rRNA
amplicon
metagenomic
sequencing,
identify
links
between
changes
in
microbial
communities
pathogens
detected
oyster
tissues
surrounding
environments.
In
addition,
adapted
BioFire®
FilmArray®,
commonly
used
hospitals,
determine
presence
within
sampled
oysters.
We
known
50
%
tested.
Within
genomic
datasets,
noted
plants
were
shared
nearby
water
sediments,
suggesting
sink-source
dynamic
surroundings.
gene
analysis
revealed
while
share
common
constituents
environments,
they
enrich
certain
bacteria
such
as
Mycoplasmatales,
Fusobacteriales,
Spirochaetales.
On
contrary,
found
viromes
harbored
same
near
equal
relative
abundances
Our
results
show
how
could
be
only
risk
estuaries
also
ecosystem-level
sentinels.
Reviews in Aquaculture,
Год журнала:
2024,
Номер
16(4), С. 1603 - 1620
Опубликована: Апрель 3, 2024
Abstract
Salmonids,
specifically
Atlantic
salmon
(
Salmo
salar
)
and
rainbow
trout
Oncorhynchus
mykiss
),
are
commonly
farmed
their
gut
microbiota
plays
important
roles
for
optimal
growth,
health,
physiology.
However,
differences
in
experimental
design,
technical
factors
bioinformatics
make
it
challenging
to
compare
the
results
from
different
studies
draw
general
conclusions
about
influence
on
fish
microbiota.
For
a
more
comprehensive
understanding
of
microbiota,
we
collected
all
publicly
accessible
16S
rRNA
gene
sequencing
data
with
clearly
stated
sample
metadata
freshwater
intestinal
contents
mucosa
sequenced
Illumina
MiSeq
platform.
A
total
783
samples
19
published
were
included
this
meta‐analysis
test
impact
technical,
environmental,
host‐accociated
factors.
This
revealed
that
tested
significantly
influenced
alpha
beta
diversities
trout.
Technical
factors,
especially
target
region
DNA
extraction
kit,
affected
diversity
larger
extent,
while
host‐associated
environmental
diet
initial
weight,
had
higher
diversity.
Salmon
abundance
Enterococcus
Staphylococcus
than
trout,
which
Weissella
Mycoplasma
.
The
fill
critical
knowledge
gap
demonstrate
methodologies
must
be
standardized
associated
host
environment
need
accounted
future
design
salmonid
experiments.
Frontiers in Physiology,
Год журнала:
2024,
Номер
14
Опубликована: Фев. 15, 2024
New
Zealand’s
green-lipped
mussel
(
Perna
canaliculus
)
is
an
ecologically
and
economically
important
species.
Marine
heatwaves
are
increasing
in
frequency
around
NZ’s
coastline,
these
events
correlated
with
increased
stress
mortality
of
some
aquaculture
This
study
aimed
to
identify
general
biomarkers
heat
P.
assess
whether
responses
differed
between
genetically
distinct
selectively
bred
mussels.
We
exposed
three
families
mussels
(families
A,
B
C)
seawater
temperature
regimes
the
laboratory:
1)
a
“control”
treatment
(ambient
12°C),
2)
26°C
challenge
subsequent
recovery
period,
3)
sustained
no
recovery.
investigated
survival,
immune
response
(hemocyte
concentration
viability,
oxidative
total
antioxidant
capacity),
hemocyte
gene
expression
gill
microbiome
during
challenges.
In
heat-stress
treatment,
family
A
had
highest
survival
rate
(42%
compared
25%
5%
for
C
B,
respectively).
Gene
levels
significantly
shifted
thermal
families,
more
dissimilar
than
C.
Family
substantially
genes
impacted
by
timepoint
other
while
very
little
genes/pathways
that
responded
stress.
Genes
related
shock
proteins
(e.g.,
AIF1,
CTSC,
TOLL8,
CASP9,
FNTA,
AHCY,
CRYAB,
PPIF)
were
upregulated
all
Microbiome
species-richness
before
heat-stress,
having
distinctly
different
flora
families.
Microbial
diversity
changed
similarly
prolonged
species
Vibrio
Campylobacter
Our
highlights
use
non-lethal
sampling
hemocytes
as
diagnostic
tool
explore
mussels,
predict
their
ocean
warming.
approach
can
potential
thermotolerant
candidates
further
selective
breeding,
which
may
increase
resilience
industry
warming
ocean.
Ecotoxicology and Environmental Safety,
Год журнала:
2023,
Номер
263, С. 115197 - 115197
Опубликована: Июль 13, 2023
High-temperature
stress
caused
by
global
climate
change
poses
a
significant
threat
to
marine
ectotherms.
This
study
investigated
the
role
of
protein
phosphorylation
modifications
in
molecular
regulation
network
under
heat
oysters,
which
are
representative
intertidal
organisms
that
experience
considerable
temperature
changes.
Firstly,
compared
extent
thermal
damage
between
two
congeneric
oyster
species,
relative
heat-tolerant
Crassostrea
angulata
(C.
angulata)
and
heat-sensitive
gigas
gigas),
sublethal
(37
°C)
for
12
h,
using
various
physiological
biochemical
methods.
Subsequently,
comparative
proteomic
phosphoproteomic
analyses
revealed
high-temperature
considerably
regulated
signal
transduction,
energy
metabolism,
synthesis,
cell
survival
apoptosis,
cytoskeleton
remodeling
through
related
receptors
kinases.
Furthermore,
kinase
A,
mitogen-activated
1,
tyrosine-protein
Src,
serine/threonine
AKT,
exhibiting
differential
modification
patterns,
were
identified
as
hub
regulators
may
enhance
glycolysis
TCA
cycle
increase
supply,
distribute
inhibit
Caspase-dependent
apoptosis
activated
endogenous
mitochondrial
cytochrome
release
maintain
cytoskeletal
stability,
ultimately
shaping
higher
resistance
C.
angulata.
represents
first
investigation
dynamics
invertebrates
stress,
reveals
mechanisms
underlying
responses
oysters
at
level,
provides
new
insights
into
understanding
phosphorylation-mediated
during
environmental
changes
predicting
adaptive
potential
context
warming.
Microorganisms,
Год журнала:
2025,
Номер
13(4), С. 825 - 825
Опубликована: Апрель 5, 2025
Bivalve
microbiota
play
a
vital
role
in
host
health,
supporting
nutrient
processing,
immunity,
and
disease
resistance.
However,
the
increasing
hypoxia
Chilean
coastal
waters,
caused
by
climate
change
eutrophication,
threatens
to
disrupt
this
microbial
balance,
potentially
promoting
pathogens
impairing
essential
functions.
Mytilus
chilensis
is
vulnerable
hypoxia-reoxygenation
cycles,
yet
effects
on
its
remain
poorly
understood.
This
study
investigates
impact
of
structure
functional
potential
communities
residing
gills
digestive
glands
M.
chilensis.
Employing
full-length
16S
rRNA
gene
sequencing,
we
explored
hypoxia’s
diversity
capacity.
Our
results
revealed
significant
alterations
composition,
with
shift
towards
facultative
anaerobes
thriving
low
oxygen
environments.
Notably,
there
was
decrease
dominant
bacterial
taxa
such
as
Rhodobacterales,
while
opportunistic
Vibrio
Aeromonas
exhibited
increased
abundance.
Functional
analysis
indicated
decline
critical
functions
associated
metabolism
immune
support,
jeopardizing
health
survival
host.
sheds
light
intricate
interactions
between
host-associated
environmental
stressors,
underlining
importance
managing
face
aquaculture
practices.
