Microorganisms,
Год журнала:
2023,
Номер
11(8), С. 1949 - 1949
Опубликована: Июль 30, 2023
How
bacteria
respond
at
the
systems
level
to
both
genetic
and
environmental
perturbations
imposed
same
time
is
one
fundamental
yet
open
question
in
biology.
Bioengineering
or
synthetic
biology
provides
an
ideal
system
for
studying
such
responses,
as
engineered
strains
always
have
changes
opposed
wildtypes
are
grown
conditions
which
often
change
during
growth
maximal
yield
of
desired
products.
So,
were
used
address
outstanding
question.
Two
Bacillus
subtilis
(MT1
MT2)
created
previously
overproduction
N-acetylglucosamine
(GlcNAc),
environment
with
a
carbon
shift
from
glucose
xylose
culture
system.
We
had
four
groups:
(1)
wildtype
(WT)
t1;
(2)
WT
t2;
(3)
mutant
(MT1)
(4)
MT1
t2.
By
measuring
transcriptomes
metabolomes,
we
found
that
GlcNAc-producing
mutants,
particularly
MT2,
higher
than
but
displayed
smaller
maximum
rate
wildtype,
despite
reaching
carrying
capacity.
Underlying
observed
growth,
pathways
leading
gene
expression
associated
metabolite
concentrations
t1
bioenergetics,
there
was
energy
supply
terms
ATP
GTP,
state
metric
timepoints.
Additionally,
most
top
key
precursor
metabolites
equally
abundant
either
Besides
that,
prominent
feature
high
consistency
between
transcriptomics
metabolomics
revealing
response.
First,
metabolomes
revealed
PCA
clusters
groups.
Second,
important
functions
enriched
by
overlapped,
amino
acid
metabolism
ABC
transport.
Strikingly,
these
overlapped
those
genes
showing
(positive
negative)
correlation
metabolites.
Furthermore,
also
KEGG
identified
using
weighted
coexpression
network
analysis.
All
findings
suggest
responses
simultaneous
well
coordinated
metabolic
transcriptional
levels:
they
rely
heavily
on
core
does
not
differ
much,
while
transport
important.
This
serves
design
guide
bioengineering,
biology,
Microbial Cell Factories,
Год журнала:
2024,
Номер
23(1)
Опубликована: Июль 25, 2024
The
remediation
of
emerging
contaminants
presents
a
pressing
environmental
challenge,
necessitating
innovative
approaches
for
effective
mitigation.
This
review
article
delves
into
the
untapped
potential
soil
microbial
communities
in
bioremediation
contaminants.
Bioremediation,
while
promising
method,
often
proves
time-consuming
and
requires
deep
comprehension
intricacies
enhancement.
Given
challenges
presented
by
inability
to
culture
many
these
microorganisms,
conventional
methods
are
inadequate
achieving
this
goal.
While
omics-based
provide
an
approach
understanding
fundamental
aspects,
processes,
connections
among
microorganisms
that
essential
improving
strategies.
By
exploring
latest
advancements
omics
technologies,
aims
shed
light
on
how
can
unlock
hidden
capabilities
communities,
paving
way
more
efficient
sustainable
solutions.
Frontiers in Bioengineering and Biotechnology,
Год журнала:
2024,
Номер
12
Опубликована: Сен. 19, 2024
The
perpetual
release
of
natural/synthetic
pollutants
into
the
environment
poses
major
risks
to
ecological
balance
and
human
health.
Amongst
these,
contaminants
emerging
concern
(CECs)
are
characterized
by
their
recent
introduction/detection
in
various
niches,
thereby
causing
significant
hazards
necessitating
removal.
Pharmaceuticals,
plasticizers,
cyanotoxins
pesticides
groups
CECs
that
highly
toxic
found
occur
compartments
biosphere.
sources
these
compounds
can
be
multipartite
including
industrial
discharge,
improper
disposal,
excretion
unmetabolized
residues,
eutrophication
etc
.,
while
fate
persistence
determined
factors
such
as
physico-chemical
properties,
environmental
conditions,
biodegradability
hydrological
factors.
resultant
exposure
microbiota
has
imposed
a
selection
pressure
resulted
evolution
metabolic
pathways
for
biotransformation
and/or
utilization
sole
source
carbon
energy.
Such
microbial
degradation
phenotype
exploited
clean-up
from
environment,
offering
cost-effective
eco-friendly
alternative
abiotic
methods
removal,
mitigating
toxicity.
However,
efficient
bioprocess
development
bioremediation
strategies
requires
extensive
understanding
individual
components
pathway
gene
clusters,
proteins/enzymes,
metabolites
associated
regulatory
mechanisms.
“Omics”
“Meta-omics”
techniques
aid
providing
crucial
insights
complex
interactions
functions
well
community,
enabling
more
effective
targeted
bioremediation.
Aside
natural
isolates,
engineering
approaches
employ
application
genetic
enhance
diversity
rates.
integration
omics
data
will
further
developing
systemic-level
strategies,
optimising
process.
This
review
describes
bacterial
catabolic
pathways,
genetics,
four
CECs:
pharmaceuticals,
cyanotoxins,
pesticides.
Microorganisms,
Год журнала:
2023,
Номер
11(9), С. 2163 - 2163
Опубликована: Авг. 26, 2023
Overuse
of
pesticides
in
agricultural
soil
and
dye-polluted
effluents
severely
contaminates
the
environment
is
toxic
to
animals
humans
making
their
removal
from
essential.
The
present
study
aimed
assess
biodegradation
(cypermethrin
(CYP)
imidacloprid
(IMI)),
dyes
(malachite
green
(MG)
Congo
red
(CR))
using
biofilms
bacteria
isolated
pesticide-contaminated
dye
effluents.
Biofilms
indigenous
bacteria,
i.e.,
Bacillus
thuringiensis
2A
(OP554568),
Enterobacter
hormaechei
4A
(OP723332),
sp.
5A
(OP586601),
cereus
6B
(OP586602)
individually
mixed
culture
were
tested
against
CYP
IMI.
Lysinibacillus
sphaericus
AF1
(OP589134),
CF3
(OP589135)
DF4
(OP589136)
for
ability
degrade
dyes.
biofilm
a
B.
+
(P7)
showed
46.2%
degradation
compared
E.
(P11),
which
significantly
high
(70.0%)
Regarding
biodegradation,
(D6)
86.76%
MG,
was
L.
(D4)
that
degraded
only
30.78%
CR.
UV-VIS
spectroscopy
revealed
major
peaks
at
224
nm,
263
581
nm
436
CYP,
IMI,
MG
CR,
respectively,
completely
disappeared
after
treatment
with
bacterial
biofilms.
Fourier
transform
infrared
(FTIR)
analysis
appearance
new
metabolites
disappearance
peak
control
spectrum
treatment.
Thin
layer
chromatography
(TLC)
also
confirmed
CR
into
several
control.
demonstrates
potential
biofilm-forming
pesticide-polluted
This
first
report
demonstrating
biofilm-mediated
bio-degradation
utilizing
effluent
flora
Multan
Sheikhupura,
Punjab,
Pakistan.
