International Journal of Molecular Sciences,
Год журнала:
2024,
Номер
25(21), С. 11643 - 11643
Опубликована: Окт. 30, 2024
Stress-inducible
heat
shock
protein
70
(Hsp70),
which
functions
as
a
molecular
chaperone
and
is
frequently
overexpressed
in
different
cancer
cell
types,
present
on
the
surface
of
tumor
cells
actively
released
into
circulation
free
extracellular
lipid
vesicle-associated
forms.
Since
exact
pathomechanism
endometriosis
has
not
yet
been
elucidated
(although
it
associated
with
development
endometrial
ovarian
cancer),
we
asked
whether
Hsp70
circulating
endometriotic
(CECs)
reflect
presence
endometriosis.
Therefore,
levels
microvesicle-associated
were
measured
using
Hsp70-exo
ELISA,
CECs
peripheral
blood
patients
was
determined
membrane
(mHsp70)
EpCAM
monoclonal
antibody
(mAb)-based
bead
isolation
approaches.
Isolated
further
characterized
by
immunofluorescence
reagents
directed
against
cytokeratin
(epithelial
marker),
CD45
(leukocyte
CD105/CD44
(mesenchymal
stemness
markers)
comparative
RNA
analysis.
Similar
to
situation
cancer,
elevated
histologically
proven
compared
healthy
control
cohort,
significantly
lesions
outside
uterine
cavity.
Moreover,
could
be
isolated
cmHsp70.1
mAb-based,
lesser
extent
approach
all
endometriosis,
highest
counts
obtained
mHsp70-targeting
procedure
extra-uterine
involvement.
The
longevity
culture
expression
cytokeratins
CD105
CD44,
together
differentially
expressed
genes
related
epithelial-to-mesenchymal
transition
(EMT),
revealed
similarities
between
mHsp70-expressing
(CTCs)
suggest
mesenchymal
stem
origin.
These
findings
support
involvement
mHsp70-positive
cell-like
lesions.
In
summary,
serve
liquid
biopsy
markers
for
help
elucidate
underlying
disease.
Biomedicine & Pharmacotherapy,
Год журнала:
2023,
Номер
166, С. 115337 - 115337
Опубликована: Сен. 4, 2023
The
fourth
common
reason
of
death
among
patients
is
gastric
cancer
(GC)
and
it
a
dominant
tumor
type
in
Ease
Asia.
One
the
problems
GC
therapy
chemoresistance.
Cisplatin
(CP)
platinum
compound
that
causes
DNA
damage
reducing
progression
viability
cells.
However,
due
to
hyperactivation
drug
efflux
pumps,
dysregulation
genes
interactions
microenvironment,
cells
can
develop
resistance
CP
chemotherapy.
current
review
focuses
on
emergence
with
emphasizing
molecular
pathways,
pharmacological
compounds
for
reversing
chemoresistance
role
nanostructures.
Changes
cell
mechanisms
such
as
upregulation
pro-survival
autophagy
prevent
CP-mediated
apoptosis
results
resistance.
Moreover,
increase
metastasis
via
EMT
induction
induces
Dysregulation
pathways
PTEN,
PI3K/Akt,
Nrf2
others
result
changes
response
Non-coding
RNAs
determine
application
activity
distinct
sensitivity
Due
efficacy
exosomes
transferring
bioactive
molecules
RNA
cells,
also
newest
progresses
overcoming
nanoplatforms
delivery
they
accumulation
at
site
by
suppressing
carcinogenic
factors
biological
barriers,
toxicity
Biomedicines,
Год журнала:
2023,
Номер
11(8), С. 2276 - 2276
Опубликована: Авг. 16, 2023
Heat
shock
protein
70
(Hsp70)
is
frequently
overexpressed
in
many
different
tumor
types.
However,
Hsp70
has
also
been
shown
to
be
selectively
presented
on
the
plasma
membrane
of
cells,
but
not
normal
and
this
form
(mHsp70)
could
considered
a
universal
biomarker.
Since
viable,
mHsp70-positive
cells
actively
release
lipid
micro-vesicles,
we
investigated
utility
circulation
as
biomarker
its
potential
an
early
predictive
marker
therapeutic
failure.
We
have
evaluated
mHsp70
target
for
isolation
enumeration
circulating
(CTCs)
patients
with
entities.
Circulating
vesicular
levels
were
measured
peripheral
blood
compHsp70
ELISA.
CTCs
isolated
using
cmHsp70.1
EpCAM
monoclonal
antibody
(mAb)-based
bead
approaches
characterized
by
immunohistochemistry
cytokeratin
CD45-specific
antibodies.
In
two
out
35
exhibiting
failure
years
after
initial
diagnosis
non-metastatic
breast
cancer,
progressively
increasing
had
already
observed
during
therapy,
whereas
without
subsequent
recurrence
remained
unaltered.
With
regards
CTC
from
tumors,
mAb-based
selection
system
appears
superior
approach.
Extracellular
can
therefore
serve
early-stage
tumors
various
diseases.
Translational Oncology,
Год журнала:
2023,
Номер
40, С. 101846 - 101846
Опубликована: Дек. 1, 2023
The
treatment
of
cancer
patients
has
been
mainly
followed
using
chemotherapy
and
it
is
a
gold
standard
in
improving
prognosis
survival
rate
patients.
Oxaliplatin
(OXA)
third-platinum
anti-cancer
agent
that
reduces
DNA
synthesis
cells
to
interfere
with
their
growth
cell
cycle
progression.
In
spite
promising
results
OXA
chemotherapy,
the
process
drug
resistance
made
some
challenges.
commonly
applied
colorectal
(CRC)
as
malignancy
gastrointestinal
tract
when
CRC
increase
proliferation
metastasis,
they
can
obtain
chemotherapy.
A
number
molecular
factors
such
CHK2,
SIRT1,
c-Myc,
LATS2
FOXC1
have
considered
regulators
response
cells.
non-coding
RNAs
are
able
function
master
regulator
other
pathways
modulating
resistance.
There
close
association
between
mechanisms
apoptosis,
autophagy,
glycolysis
EMT
resistance,
so
apoptosis
inhibition,
pro-survival
autophagy
induction
stimulation
induce
anti-tumor
compounds
including
astragaloside
IV,
resveratrol
nobiletin
enhance
sensitivity
Nanoparticles
for
increasing
potential
suppression
reversing
employed
These
subjects
covered
this
review
article
shed
light
on
resulting
American Journal of Cancer Research,
Год журнала:
2024,
Номер
14(8), С. 3935 - 3961
Опубликована: Янв. 1, 2024
The
nuclear
factor-erythroid
2-related
factor
2
(Nrf2)
is
able
to
control
the
redox
balance
in
cells
responding
oxidative
damage
and
other
stress
signals.
Nrf2
upregulation
can
elevate
levels
of
antioxidant
enzymes
support
against
death.
In
spite
protective
function
physiological
conditions,
stimulation
cancer
has
been
favour
tumorigenesis.
Since
dysregulation
molecular
pathways
mutations/deletions
are
common
tumors,
be
a
promising
therapeutic
target.
overexpression
prevent
cell
death
tumor
by
increasing
survival
rate
cells,
ensures
carcinogenesis.
Moreover,
induction
promote
invasion
metastasis
cells.
stimulates
EMT
increase
metastasis.
Furthermore,
regarding
Nrf2,
its
triggers
chemoresistance.
natural
products
regulate
therapy
reverse
drug
resistance.
nanostructures
specifically
target
signaling
therapy.
current
review
discusses
potential
proliferation,
Then,
capacity
for
suppressing
Nrf2-mediated
progression
discussed.
Bladder
cancer
(BC)
is
a
prevalent
type
of
tumor
in
the
urinary
system,
and
it
has
been
discovered
that
long
non-coding
RNA
(lncRNA)
plays
significant
role
its
occurrence
development.
However,
thus
far,
no
reports
have
published
on
involvement
LINC00461
BC.
Here,
we
found
levels
were
upregulated
BC
tissues
cell
lines.
Besides,
knockdown
inhibited
proliferation,
migration,
invasion
through
epithelial-mesenchymal
transition
(EMT),
slowed
down
growth
vivo.
Moreover,
regulated
HNRNPUL1
expression
miR-518b
sponge
activity,
miR-518
inhibitor
could
reverse
inhibitory
effects
EMT.
Our
results
suggest
may
serve
as
potential
biomarker
therapeutic
target
for
Reprogramming
of
cellular
metabolism
in
tumors
promoted
the
epithelial-mesenchymal
transition
(EMT)
process
and
established
immune-suppressive
tumor
microenvironments
(iTME),
leading
to
drug
resistance
progression.
Therefore,
remodeling
cells
was
a
promising
strategy
overcome
drug-resistant
tumors.
Herein,
CD276
MTHFD2
were
identified
as
specific
marker
therapeutic
target,
respectively,
for
targeting
sunitinib-resistant
clear
cell
renal
carcinoma
(ccRCC)
its
cancer
stem
(CSC)
population.
The
blockade
confirmed
via
folate-nucleotide
metabolism.
Moreover,
manganese
dioxide
nanoparticle
proven
here
by
high-throughput
metabolome
be
capable
γ-aminobutyric
acid
(GABA)
reconstruct
iTME.
Based
on
these
findings,
engineered
CD276-CD133
dual-targeting
biomimetic
nanovesicle
EMφ-siMTHFD2-MnO
