bioRxiv (Cold Spring Harbor Laboratory),
Год журнала:
2024,
Номер
unknown
Опубликована: Июнь 25, 2024
Abstract
Phenol
isopropylated
phosphates
(IPP)
are
an
additive
organophosphate
flame
retardant
(OPFR)
which
has
been
extensively
used
in
furniture,
electronics,
automobiles,
plastics,
and
children’s
products
to
slow
down
the
spread
of
fire.
The
processing
distribution
IPP-containing
have
prohibited
but
its
continuous
leaching
from
end
use
retained
concern
toxicity.
present
study
was
designed
evaluate
IPP-induced
developmental
toxicity
using
zebrafish
embryos.
We
first
conducted
range
finding
experiments
with
embryonic
exposures
0-200
μM
IPP
6
120
h
post
fertilization
found
significant
morphological
impacts
like
pericardial
edema,
yolk
sac
edema
spinal
curvature
at
higher
concentrations.
For
behavioral
readouts,
we
performed
larval
photomotor
response
(LPR)
assay
sublethal
concentrations
observed
hypoactive
locomotory
behavior
exposed
larvae.
Following
this,
relying
on
secondary
analyses
our
whole
embryo
mRNA-seq
data,
conducted-1)
retinoic
acid
receptor
(RAR)
signaling
2)
DNA
methylation
assays.
In
vitro
for
RA
receptors
indicate
that
significantly
inhibits
RARα,
not
RARβ
RARγ.
Whole-mount
immunohistochemistry
5-methylcytosine
global
showed
hypermethylation
situ
.
co-exposure
studies
a
methylome
modifier
5-azacytidine
(Aza-c
inhibitor)
or
activators
assess
if
LPR
phenotypes
were
mitigated
by
co-exposures.
Data
Aza-c
co-exposures
partially
reversed
hypoactivity
hypermethylation,
as
well
AM580
(an
RARα
activator)
able
reverse
hypoactivity.
Finally,
based
RNA-seq
hypothesized
affects
development
brain
eyes.
Firstly,
eyes,
did
find
any
effects.
Then,
mRNA
sequencing
dissected
brains
2
135
differentially
expressed
genes,
respectively.
Gene
ontology
revealed
affect
phototransduction,
voltage
gated
ion
channels,
synaptic
neurotransmitter
signaling.
Collectively,
data
shows
induces
abnormalities
disrupts
photo
motor
response,
potentially
through
methylomic
regulation.
gene
expression
within
developing
eye,
establishing
transmission,
vision
muscle
contraction
potential
causative
factor
responses.
Environment International,
Год журнала:
2023,
Номер
172, С. 107745 - 107745
Опубликована: Янв. 10, 2023
As
a
substitute
for
polybrominated
diphenyl
ethers
(PBDEs),
organophosphate
flame
retardant
triphenyl
phosphate
(TPhP)
is
widely
used
in
our
daily
products
and
diffusely
exists
living
surroundings,
but
there
paucity
of
information
concerning
its
neurodevelopmental
toxicity.
Herein,
we
investigated
the
effects
TPhP
exposure
on
developmental
parameters,
locomotor
behavior,
oxidative
stress,
apoptosis
transcriptional
levels
zebrafish
at
different
stages,
so
as
to
explore
neural
development
underlying
molecular
mechanisms.
concentration
gradient
reduced
survival
rate,
hatchability,
heart
body
length
eye
distance
embryos/larvae,
caused
malformations
larvae.
also
leads
abnormal
behaviors,
such
swimming
speed,
impaired
panic
avoidance
reflex
high
light
stimulation.
ROS
accumulation
96
hpf
larvae
induced
Nrf2-antioxidant
response
zebrafish.
In
addition,
further
activated
mitochondrial
signaling
pathways,
which
affected
region,
resulting
visual
impairment.
Neurodevelopmental
(mbpa,
syn2a,
foxo3a
pax6a),
Retinoid
acid
metabolism
(cyp26a1,
raraa,
rbp5,
rdh1,
crabp1a
rbp2a)
apoptosis-related
genes
(bcl2a,
baxa
casp9)
revealed
mechanism
behavior
phenotypic
symptoms,
indicated
that
are
more
sensitive
than
7
dpf
Thus,
present
study,
neurotoxic
early
life
stages
zebrafish,
impairments
by
attributed
co-regulation
visuomotor
dysfunction
neuro-related
genes.
These
results
suggest
safety
organisms
even
humans
needs
be
studied.
Comparative Biochemistry and Physiology Part C Toxicology & Pharmacology,
Год журнала:
2023,
Номер
270, С. 109645 - 109645
Опубликована: Май 5, 2023
Plastics,
long-chain
artificial
polymers,
are
used
worldwide
with
a
global
production
of
350
million
tonnes
per
year.
Various
degradation
processes
transform
plastics
into
smaller
fragments
divided
micro,
meso
and
macroplastics.
In
various
industries,
such
as
construction,
certain
plastic
additives
to
improve
flexibility
enhance
performance.
Plastic
include
phthalates
(PAE),
dibutyl
phthalate
(DPB)
diethyl
(DEP).
Due
the
use
additives,
these
small
different
shapes
colours
present
in
all
environmental
compartments.
For
their
characteristics,
PAEs
can
be
introduced
particularly
by
ingestion,
inhalation
dermal
absorption.
They
accumulate
human
body,
where
they
have
already
been
identified
blood,
amniotic
fluid
urine.
The
purpose
this
review
is
gather
effects
that
on
systems
body.
Being
endocrine
disruptors,
erythrocytes
how
considered
targets
for
xenobiotics
analysed.
influence
reproductive
system
was
also
examined.
Phthalates
therefore
often
overused.
properties,
reach
tissues
negative
impact
health.
aim
give
an
overview
presence
hazards.
Therefore,
should
reduced,
replaced
disposal
improved.
Environmental Science & Technology,
Год журнала:
2023,
Номер
57(22), С. 8189 - 8212
Опубликована: Май 17, 2023
The
global
birth
rate
has
recently
shown
a
decreasing
trend,
and
exposure
to
environmental
pollutants
been
identified
as
potential
factor
affecting
female
reproductive
health.
Phthalates
have
widely
used
plasticizers
in
plastic
containers,
children's
toys,
medical
devices,
their
ubiquitous
presence
endocrine-disrupting
already
raised
particular
concerns.
Phthalate
linked
various
adverse
health
outcomes,
including
diseases.
Given
that
many
phthalates
are
gradually
being
banned,
growing
number
of
phthalate
alternatives
becoming
popular,
such
di(isononyl)
cyclohexane-1,2-dicarboxylate
(DINCH),
di(2-ethylhexyl)
adipate
(DEHA),
terephthalate
(DEHTP),
they
beginning
wide
range
effects.
Studies
may
disrupt
function
by
altering
the
estrous
cycle,
causing
ovarian
follicular
atresia,
prolonging
gestational
which
raises
concerns
about
risks.
Herein,
we
summarize
effects
common
different
models,
levels
influence
system,
on
impairment,
pregnancy
offspring
development.
Additionally,
scrutinize
hormone
signaling,
oxidative
stress,
intracellular
signaling
explore
underlying
mechanisms
action
health,
because
these
chemicals
affect
tissues
directly
or
indirectly
through
endocrine
disruption.
declining
trends
capacity
ability
negatively
impact
more
comprehensive
study
is
needed
understand
human
body
mechanisms.
These
findings
an
important
role
improving
turn
complications
during
pregnancy.
