Geraniin Alleviates Mouse Laser‐Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang‐(1–7)/MasR/IL‐10 Pathway DOI Creative Commons

Hongyi Lu,

Qi Cai, Lele Li

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(23)

Опубликована: Дек. 1, 2024

ABSTRACT Anti‐vascular endothelial growth factor (VEGF) drugs suppress choroidal neovascularisation (CNV), thus improving vision. However, some patients may have a poor response or develop resistance to anti‐VEGF drugs. Geraniin (GE), polyphenol isolated from an herb called Phyllanthus amarus , possesses anti‐angiogenic properties. This study aimed explore the mechanism of action GE in CNV. was found activate angiotensin‐converting enzyme 2 (ACE2)/angiotensin 1–7 (Ang‐[1–7])/MAS1 proto‐oncogene, G protein‐coupled receptor (MasR)/interleukin‐10 (IL‐10) pathway hypoxic human cells (HCECs) vitro and mouse models laser‐induced CNV vivo. Activation ACE2/Ang‐(1–7)/MasR/IL‐10 by attenuated proliferative, migratory, tube‐forming abilities HCECs prevented development mice. Notably, did not cause ocular systemic toxicity mice with These findings suggest that alleviates activating (CECs).

Язык: Английский

(+)-a-Longipinene alleviates murine laser-induced choroidal neovascularization: Evidence from in vivo and in vitro experiments DOI
Manhui Zhu, Min Wu, Aimin Yang

и другие.

International Immunopharmacology, Год журнала: 2025, Номер 159, С. 114913 - 114913

Опубликована: Май 23, 2025

Язык: Английский

Процитировано

0
Comparative analysis of respiratory metabolism, blood physiology, antioxidant capacity, and hypoxia-related gene expression in Snowy White chickens raised at high and low altitudes DOI Creative Commons
Junyang Liu, Felix Kwame Amevor, Ke Qi

и другие.

Poultry Science, Год журнала: 2025, Номер unknown, С. 105378 - 105378

Опубликована: Май 1, 2025

Язык: Английский

Процитировано

0
An Electrospun DFO-Loaded Microsphere/SAIB System Orchestrates Angiogenesis–Osteogenesis Coupling via HIF-1α Activation for Vascularized Bone Regeneration DOI Open Access

Xujia Shan,

Xiaoyan Yuan, Xiaohong Wu

и другие.

Polymers, Год журнала: 2025, Номер 17(11), С. 1538 - 1538

Опубликована: Май 31, 2025

This study developed electrosprayed deferoxamine (DFO)-loaded poly(lactic-co-glycolic acid) microspheres (DFO-MS) combined with a sucrose acetate isobutyrate (SAIB) depot (DFO-MS@SAIB) for bone-defect repair, targeting the coordinated regulation of angiogenesis and osteogenesis in vascularized bone regeneration—where new blood vessels support functional integration. In vitro/in vivo evaluations confirmed its dual pro-angiogenic pro-osteogenic effects via HIF-1α pathway activation. Background/Objectives: Emerging evidence underscores indispensability vascularization clinical challenge exacerbated by limited intrinsic healing capacity. While autologous grafts growth-factor-based strategies remain mainstream, their utility is constrained donor-site morbidity, transient bioactivity, poor spatiotemporal control over angiogenic–osteogenic coupling. Here, we leveraged DFO, hypoxia-mimetic stabilizer angiogenic potential, to engineer an injectable DFO-MS@SAIB depot. system was designed achieve sustained DFO release, thereby synchronizing vascular network formation mineralized tissue regeneration critical-sized defects. Methods: DFO-MS were fabricated electrospraying SAIB (DFO-MS@S) form sustained-release Their physicochemical properties, including morphology, encapsulation efficiency, degradation, release kinetics, rheology, systematically characterized. vitro, capacity HUVECs co-cultured evaluated; conditioned then BMSCs assess BMSCs’ cytocompatibility osteogenic differentiation. rat calvarial defect model evaluated using micro-CT, histology, immunohistochemistry. Results: The achieved stimulating HUVEC proliferation, migration, tubulogenesis. Transwell co-culture model, pretreated promoted BMSC migration differentiation paracrine signaling involving endothelial-secreted factors (e.g., VEGF). activation upregulated markers (ALP, Col1a1, OCN), while experiments demonstrated enhanced regeneration, significantly increased volume/total volume (BV/TV) area compared controls. Conclusion: promotes HIF-1α-mediated signaling. Its angiogenesis–osteogenesis coupling effect facilitates offering translatable strategy repair.

Язык: Английский

Процитировано

0
Geraniin Alleviates Mouse Laser‐Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang‐(1–7)/MasR/IL‐10 Pathway DOI Creative Commons

Hongyi Lu,

Qi Cai, Lele Li

и другие.

Journal of Cellular and Molecular Medicine, Год журнала: 2024, Номер 28(23)

Опубликована: Дек. 1, 2024

ABSTRACT Anti‐vascular endothelial growth factor (VEGF) drugs suppress choroidal neovascularisation (CNV), thus improving vision. However, some patients may have a poor response or develop resistance to anti‐VEGF drugs. Geraniin (GE), polyphenol isolated from an herb called Phyllanthus amarus , possesses anti‐angiogenic properties. This study aimed explore the mechanism of action GE in CNV. was found activate angiotensin‐converting enzyme 2 (ACE2)/angiotensin 1–7 (Ang‐[1–7])/MAS1 proto‐oncogene, G protein‐coupled receptor (MasR)/interleukin‐10 (IL‐10) pathway hypoxic human cells (HCECs) vitro and mouse models laser‐induced CNV vivo. Activation ACE2/Ang‐(1–7)/MasR/IL‐10 by attenuated proliferative, migratory, tube‐forming abilities HCECs prevented development mice. Notably, did not cause ocular systemic toxicity mice with These findings suggest that alleviates activating (CECs).

Язык: Английский

Процитировано

0