(+)-a-Longipinene alleviates murine laser-induced choroidal neovascularization: Evidence from in vivo and in vitro experiments
International Immunopharmacology,
Год журнала:
2025,
Номер
159, С. 114913 - 114913
Опубликована: Май 23, 2025
Язык: Английский
Comparative analysis of respiratory metabolism, blood physiology, antioxidant capacity, and hypoxia-related gene expression in Snowy White chickens raised at high and low altitudes
Poultry Science,
Год журнала:
2025,
Номер
unknown, С. 105378 - 105378
Опубликована: Май 1, 2025
Язык: Английский
An Electrospun DFO-Loaded Microsphere/SAIB System Orchestrates Angiogenesis–Osteogenesis Coupling via HIF-1α Activation for Vascularized Bone Regeneration
Polymers,
Год журнала:
2025,
Номер
17(11), С. 1538 - 1538
Опубликована: Май 31, 2025
This
study
developed
electrosprayed
deferoxamine
(DFO)-loaded
poly(lactic-co-glycolic
acid)
microspheres
(DFO-MS)
combined
with
a
sucrose
acetate
isobutyrate
(SAIB)
depot
(DFO-MS@SAIB)
for
bone-defect
repair,
targeting
the
coordinated
regulation
of
angiogenesis
and
osteogenesis
in
vascularized
bone
regeneration—where
new
blood
vessels
support
functional
integration.
In
vitro/in
vivo
evaluations
confirmed
its
dual
pro-angiogenic
pro-osteogenic
effects
via
HIF-1α
pathway
activation.
Background/Objectives:
Emerging
evidence
underscores
indispensability
vascularization
clinical
challenge
exacerbated
by
limited
intrinsic
healing
capacity.
While
autologous
grafts
growth-factor-based
strategies
remain
mainstream,
their
utility
is
constrained
donor-site
morbidity,
transient
bioactivity,
poor
spatiotemporal
control
over
angiogenic–osteogenic
coupling.
Here,
we
leveraged
DFO,
hypoxia-mimetic
stabilizer
angiogenic
potential,
to
engineer
an
injectable
DFO-MS@SAIB
depot.
system
was
designed
achieve
sustained
DFO
release,
thereby
synchronizing
vascular
network
formation
mineralized
tissue
regeneration
critical-sized
defects.
Methods:
DFO-MS
were
fabricated
electrospraying
SAIB
(DFO-MS@S)
form
sustained-release
Their
physicochemical
properties,
including
morphology,
encapsulation
efficiency,
degradation,
release
kinetics,
rheology,
systematically
characterized.
vitro,
capacity
HUVECs
co-cultured
evaluated;
conditioned
then
BMSCs
assess
BMSCs’
cytocompatibility
osteogenic
differentiation.
rat
calvarial
defect
model
evaluated
using
micro-CT,
histology,
immunohistochemistry.
Results:
The
achieved
stimulating
HUVEC
proliferation,
migration,
tubulogenesis.
Transwell
co-culture
model,
pretreated
promoted
BMSC
migration
differentiation
paracrine
signaling
involving
endothelial-secreted
factors
(e.g.,
VEGF).
activation
upregulated
markers
(ALP,
Col1a1,
OCN),
while
experiments
demonstrated
enhanced
regeneration,
significantly
increased
volume/total
volume
(BV/TV)
area
compared
controls.
Conclusion:
promotes
HIF-1α-mediated
signaling.
Its
angiogenesis–osteogenesis
coupling
effect
facilitates
offering
translatable
strategy
repair.
Язык: Английский
Geraniin Alleviates Mouse Laser‐Induced Choroidal Neovascularisation by Inhibiting Choroidal Endothelial Cell ACE2/Ang‐(1–7)/MasR/IL‐10 Pathway
Journal of Cellular and Molecular Medicine,
Год журнала:
2024,
Номер
28(23)
Опубликована: Дек. 1, 2024
ABSTRACT
Anti‐vascular
endothelial
growth
factor
(VEGF)
drugs
suppress
choroidal
neovascularisation
(CNV),
thus
improving
vision.
However,
some
patients
may
have
a
poor
response
or
develop
resistance
to
anti‐VEGF
drugs.
Geraniin
(GE),
polyphenol
isolated
from
an
herb
called
Phyllanthus
amarus
,
possesses
anti‐angiogenic
properties.
This
study
aimed
explore
the
mechanism
of
action
GE
in
CNV.
was
found
activate
angiotensin‐converting
enzyme
2
(ACE2)/angiotensin
1–7
(Ang‐[1–7])/MAS1
proto‐oncogene,
G
protein‐coupled
receptor
(MasR)/interleukin‐10
(IL‐10)
pathway
hypoxic
human
cells
(HCECs)
vitro
and
mouse
models
laser‐induced
CNV
vivo.
Activation
ACE2/Ang‐(1–7)/MasR/IL‐10
by
attenuated
proliferative,
migratory,
tube‐forming
abilities
HCECs
prevented
development
mice.
Notably,
did
not
cause
ocular
systemic
toxicity
mice
with
These
findings
suggest
that
alleviates
activating
(CECs).
Язык: Английский