Blue Light Irradiation Elicits Senescence of Corneal Endothelial Cells In Vitro by Provoking Energy Crisis, Inflammasome Assembly and DNA Damage DOI
Xin Zheng, Guojian Jiang, Ting‐Jun Fan

и другие.

Current Eye Research, Год журнала: 2025, Номер unknown, С. 1 - 12

Опубликована: Май 8, 2025

The blue light from the digital screens endangers visual system among which corneas at outmost of eyes are vulnerable to irradiation. Therein, human corneal endothelial (HCE) cells crucial maintain transparency and their damage leads HCE decompensation resulting in blindness ultimately. Thus, understanding phototoxic effects on underlying mechanisms is important for taking measures protect vision clarity blue-light hazard. We pulse-irradiated cell line logarithmic phase 3 passages using 440 nm examined levels reactive oxygen species (ROS), ATP, nicotinamide adenine dinucleotide (NAD+) autophagy cytochemistry assay investigate alterations energy metabolism. Moreover, we γH2AX+ immunofluorescence expression poly(ADP-Ribose)polymerase1 (PARP1) western blotting degrees DNA repair. also monitored inflammasome senescence associated secretory phenotypes (SASPs) interleukin (IL)-8, IL-1β IL-6 qPCR ELISA assembly secretion SASPs. detected senescent features with senescence-associated-β-galactosidase assay, p16 by blotting, Lamin B1 localization observation, growth EdU incorporation confluence forming time morphology relative areas microscopy observation. exhibited after blue-light-pulse-irradiation. provokes overproduction ROS decrease NAD+ leading crisis. excess injure downregulate PARP1 stable cell-cycle arrest. facilitate hypersecretion elicits via inducing crisis, arrest SASP hypersecretion.

Язык: Английский

Photoprotective Effects of Phytochemicals on Blue Light-Induced Retinal Damage: Current Evidence and Future Perspectives DOI Open Access
Wan-Ju Yeh,

Chunli Yan,

Chih‐Hsiung Wu

и другие.

Nutrients, Год журнала: 2025, Номер 17(2), С. 331 - 331

Опубликована: Янв. 17, 2025

The widespread use of light-emitting diodes (LEDs) has increased blue light (BL) exposure, raising concerns about its potential adverse effects on ocular health. Prolonged exposure to BL been implicated in the pathogenesis various retinal disorders, including age-related macular degeneration (AMD), primarily through mechanisms involving oxidative stress and inflammation mediated by overproduction reactive oxygen species (ROS). This review synthesizes current evidence photoprotective properties dietary bioactive compounds, (e.g., anthocyanins, curcumin, quercetin, myricetin, resveratrol), with a focus their mitigate BL-induced damage. Accumulating research suggests that antioxidants, particularly polyphenols, may offer benefits. These phytochemicals act neutralizing ROS enhancing retina's endogenous antioxidant capacity. Based these findings, this advocates for food-first approach future investigations, emphasizing development evidence-based recommendations bolster health risk BL-related diseases. Considering lack empirical clinical studies examining impact human health, field hazard should prioritize two key approaches: conducting large-scale epidemiological surveys implementing trials functional ingredients have demonstrated beneficial against photodamage preclinical animal studies.

Язык: Английский

Процитировано

0
Beneficial Effect of Rebamipide Eye Drops on Blue Light-Induced Oxidative Damage in the Ocular Surface DOI
Jingting Liu,

Enying Jiang,

Hyunjee Kim

и другие.

Journal of Ocular Pharmacology and Therapeutics, Год журнала: 2025, Номер unknown

Опубликована: Апрель 28, 2025

Purpose: We evaluated the capacity of rebamipide (REB) to alleviate corneal epithelial damage induced via blue light (BL) exposure. Methods: Eight-week-old C57BL/6 mice were exposed BL (410 nm, 100 J) twice daily for 10 days. The randomly divided into 5 groups: 1 untreated and 4 groups receiving exposure ± different topical treatments: alone, carboxymethylcellulose, 5% N-acetylcysteine, REB. Reactive oxygen species (ROS) levels assessed, Bcl-2-associated X protein (BAX) was analyzed. Apoptotic cells detected, inflammatory cytokine [tumor necrosis factor-alpha (TNF-α) interleukin-6 (IL-6)] measured using enzyme-linked immunosorbent assay (ELISA), histopathological changes in cornea hematoxylin eosin (H&E) staining. Results: REB group demonstrated significantly lower exposure-induced ROS (P < 0.01) BAX expression than group. number Terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL) positive 0.01). Furthermore, ELISA analysis revealed reduced TNF-α IL-6 relative Hematoxylin staining showed preservation thickness. Conclusions: Rebamipide alleviated BL-induced oxidative ocular surfaces by reducing levels, inhibiting apoptosis, suppressing expression.

Язык: Английский

Процитировано

0
Blue Light Irradiation Elicits Senescence of Corneal Endothelial Cells In Vitro by Provoking Energy Crisis, Inflammasome Assembly and DNA Damage DOI
Xin Zheng, Guojian Jiang, Ting‐Jun Fan

и другие.

Current Eye Research, Год журнала: 2025, Номер unknown, С. 1 - 12

Опубликована: Май 8, 2025

The blue light from the digital screens endangers visual system among which corneas at outmost of eyes are vulnerable to irradiation. Therein, human corneal endothelial (HCE) cells crucial maintain transparency and their damage leads HCE decompensation resulting in blindness ultimately. Thus, understanding phototoxic effects on underlying mechanisms is important for taking measures protect vision clarity blue-light hazard. We pulse-irradiated cell line logarithmic phase 3 passages using 440 nm examined levels reactive oxygen species (ROS), ATP, nicotinamide adenine dinucleotide (NAD+) autophagy cytochemistry assay investigate alterations energy metabolism. Moreover, we γH2AX+ immunofluorescence expression poly(ADP-Ribose)polymerase1 (PARP1) western blotting degrees DNA repair. also monitored inflammasome senescence associated secretory phenotypes (SASPs) interleukin (IL)-8, IL-1β IL-6 qPCR ELISA assembly secretion SASPs. detected senescent features with senescence-associated-β-galactosidase assay, p16 by blotting, Lamin B1 localization observation, growth EdU incorporation confluence forming time morphology relative areas microscopy observation. exhibited after blue-light-pulse-irradiation. provokes overproduction ROS decrease NAD+ leading crisis. excess injure downregulate PARP1 stable cell-cycle arrest. facilitate hypersecretion elicits via inducing crisis, arrest SASP hypersecretion.

Язык: Английский

Процитировано

0