Induction of Accelerated Aging in a Mouse Model

Cells, Год журнала: 2022, Номер 11(9), С. 1418 - 1418

Опубликована: Апрель 22, 2022

With the global increase of elderly population, improvement treatment for various aging-related diseases and extension a healthy lifespan have become some most important current medical issues. In order to understand developmental mechanisms aging disorders, animal models are essential conduct relevant studies. Among them, mice one prevalently used model animals studies due their high similarity humans in terms genetic background physiological structure, as well short ease reproduction. This review will discuss common emerging mouse accelerated related chronic recent years, with aim serving reference future application fundamental translational research.

Язык: Английский

---

Rutin attenuates d-galactose-induced oxidative stress in rats’ brain and liver: molecular docking and experimental approaches

Food & Function, Год журнала: 2023, Номер 14(12), С. 5728 - 5751

Опубликована: Янв. 1, 2023

Oxidative stress results from the imbalance between reactive oxygen species (ROS) production and antioxidant defence is primarily involved in aging. The current study investigated activity of rutin aging rats induced by D-galactose (D-gal) for 42 days. Rutin was orally used at doses 50 100 mg kg-1 daily. Results showed that D-gal oxidative alterations brain liver recognized via upregulation markers. In contrast, ameliorated enhancing markers such as superoxide dismutase-1, glutathione peroxidase-1, S-transferase-α. Also, significantly decreased accumulation β-galactosidase reduced expression p53, p21, Bcl-2-associated X protein (Bax), caspase-3 (CASP3), mammalian target rapamycin (mTOR) hepatic tissues. potentially attenuated these aging-related a dose-dependent manner. Moreover, markedly increased immunohistochemical β-galactosidase, 8-hydroxy-2'-deoxyguanosine, calcium-binding adapter molecule 1, glial fibrillary acidic protein, Bax, interleukin-6 Bcl2, synaptophysin, Ki67. Furthermore, molecular docking revealed exhibited high affinity to rat human caspases, PI3K/AKT/mTOR, IL-6 receptor. Finally, we can conclude supplementation be promising natural protective compound could delay maintain health.

Язык: Английский

---

Pregnane X receptor agonist nomilin extends lifespan and healthspan in preclinical models through detoxification functions

Nature Communications, Год журнала: 2023, Номер 14(1)

Опубликована: Июнь 8, 2023

Citrus fruit has long been considered a healthy food, but its role and detailed mechanism in lifespan extension are not clear. Here, by using the nematode C. elegans, we identified that nomilin, bitter-taste limoloid is enriched citrus, significantly extended animals' lifespan, healthspan, toxin resistance. Further analyses indicate this ageing inhibiting activity depended on insulin-like pathway DAF-2/DAF-16 nuclear hormone receptors NHR-8/DAF-12. Moreover, human pregnane X receptor (hPXR) was as mammalian counterpart of NHR-8/DAF-12 X-ray crystallography showed nomilin directly binds with hPXR. The hPXR mutations prevented binding blocked both cells elegans. Finally, dietary supplementation improved healthspan D-galactose- doxorubicin-induced senescent mice well male senescence accelerated prone 8 (SAMP8) mice, induced longevity gene signature similar to most interventions liver bile-duct-ligation mice. Taken together, may extend animals via activation PXR mediated detoxification functions.

Язык: Английский

---

Cow placenta extract ameliorates d-galactose-induced liver damage by regulating BAX/CASP3 and p53/p21/p16 pathways

Journal of Ethnopharmacology, Год журнала: 2024, Номер 323, С. 117685 - 117685

Опубликована: Янв. 1, 2024

Placenta is a kind of traditional Chinese medicine, known as "Ziheche", which has the function tonifying qi and blood, nourishing liver kidney. extract (PE) been used for delaying organismal aging treating various diseases. Cow placenta rich natural resource with large mass. Its composition similar to that human placenta, but it not effectively utilized. However, little about effect CPE on mice.

Язык: Английский

---

Spatial mapping of dextran sodium sulphate‐induced intestinal inflammation and its systemic effects

The FASEB Journal, Год журнала: 2025, Номер 39(4)

Опубликована: Фев. 24, 2025

Inflammatory bowel disease (IBD) is a multifactorial disease, and patients frequently experience extraintestinal manifestations affecting multiple sites. Causes of systemic inflammation remain poorly understood, but molecules originating from the intestine likely play role, with microbial host small polarizing immune cells towards pro- or anti-inflammatory phenotype. Using dextran sodium sulfate (DSS) mouse model, which mimics disrupted barrier function, dysbiosis, cell dysregulation IBD, we investigated metabolomic phenotypic changes at intestinal spatial biology approaches, mapped distribution relative abundance types across range tissues, revealing significant in DSS-treated mice. Molecules identified as contributing to statistical separation treated control mice were spatially localized within organs determine their effects on cellular phenotypes through imaging mass cytometry. This approach both molecular drivers inflammation, including several not previously implicated linked IBD inflammation. Metabolic inflammatory pathway interplay underpins determining level may aid development new targeted therapies.

Язык: Английский

---

Pomegranate polyphenol punicalagin improves learning memory deficits, redox homeostasis, and neuroinflammation in aging mice

Phytotherapy Research, Год журнала: 2023, Номер 37(9), С. 3655 - 3674

Опубликована: Апрель 24, 2023

Abstract Alzheimer's disease (AD) is an irreversible, progressive brain disorder characterized by loss of memory and cognitive dysfunction in the aged. Despite remarkable advances drug therapy, effective pharmacological interventions are rare. Punicalagin (PU) active antioxidant polyphenol found pomegranates, raspberries, blueberries, chestnuts that has attracted considerable attention owing to its strong anti‐inflammatory properties. The current study focused on neuroprotective effect PU aging mice potential mechanisms. In this study, we first evaluated protective neuro‐2a (N2a) cell damage mediated BV2 microglia‐induced neuroinflammation. vivo D‐galactose (D‐gal)‐induced model demonstrated ameliorated deficits learning prevented neuroinflammation, which was evident from decrease microglial activation astrocytosis. Furthermore, reduced levels malondialdehyde (MDA) reactive oxygen species (ROS) inhibited NLRP3 inflammasome activation, reducing inflammatory cytokines, such as interleukin‐6 (IL‐6), tumor necrosis factor‐a (TNF‐a), interleukin‐18 (IL‐18), interleukin‐1 beta (IL‐1β) both accelerated naturally senescent mouse models. effectively improved deficits, redox homeostasis mice, it could be a therapeutic agent for AD.

Язык: Английский

---

Antioxidative potential of Lactobacillus sp. in ameliorating D-galactose-induced aging

Applied Microbiology and Biotechnology, Год журнала: 2022, Номер 106(13-16), С. 4831 - 4843

Опубликована: Июль 4, 2022

Abstract Aging is a progressive, unalterable physiological degradation process of living organisms, which leads to deterioration biological function and eventually senescence. The most prevalent factor responsible for aging the accumulation damages resulting from oxidative stress dysbiosis. D-galactose-induced has become hot topic, extensive research being conducted in this area. Published literature reported that continuous administration D-galactose motor cognitive skills, resembling symptoms aging. Hence, procedure employed as model accelerated This review aims emphasize effect on various bodily organs underline role Lactobacillus sp. process, along with its anti-oxidative potential. A critical consideration describing animal models have used amending also given. Key points • D-Galactose induces via decreasing respiratory chain enzyme activity well ATP synthesis, mitochondrial dysfunction, increased ROS production. induced primarily affects brain, heart, lung, liver, kidney, skin. potential improving direct feeding Lactobacillus-fermented food.

Язык: Английский

---

D‐galactose‐induced cardiac ageing: A review of model establishment and potential interventions

Journal of Cellular and Molecular Medicine, Год журнала: 2022, Номер 26(21), С. 5335 - 5359

Опубликована: Окт. 17, 2022

Abstract Cardiovascular disease (CVD) is highly prevalent in an ageing society. The increased incidence and mortality rates of CVD are global issues endangering human health. There urgent requirement for understanding the aetiology pathogenesis developing possible interventions preventing hearts. It necessary to select appropriate models treatment methods. D‐galactose‐induced cardiac model possesses advantages low mortality, short time cost has been increasingly used study cardiovascular diseases recent years. Therefore, latest progress valuable. This review highlights potential therapeutic years by providing a comprehensive summary vivo vitro. may serve as reference literature future research on age‐related heart diseases.

Язык: Английский

---

Sodium arsenite accelerates D-galactose-induced aging in the testis of the rat: Evidence for mitochondrial oxidative damage, NF-kB, JNK, and apoptosis pathways

Toxicology, Год журнала: 2022, Номер 470, С. 153148 - 153148

Опубликована: Март 1, 2022

Язык: Английский

---

Arctium lappa L. polysaccharides alleviate oxidative stress and inflammation in the liver and kidney of aging mice by regulating intestinal homeostasis

International Journal of Biological Macromolecules, Год журнала: 2024, Номер 280, С. 135802 - 135802

Опубликована: Сен. 19, 2024

Язык: Английский

---