Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 18, 2023
Abstract
In
this
study,
two
different
concentrations
of
matrix
certified
reference
materials
(CRMs)
were
produced
for
the
accurate
measurement
aflatoxin
M1(AFM1)
in
milk
powder((GBW(E)
100552,
GBW(E)
100553)).
The
raw
material
was
obtained
by
feeding
cows
with
positive
drugs.
homogeneity,
stability
and
characterization
CRMs
examined
liquid
chromatography
tandem
mass
spectrometry
isotope-labeled
internal
standard
method.
value
low
concentration
AFM1
powder
2.45
µg/kg
an
uncertainty
0.43
(coverage
factor
k
=
2,
at
95%
confidence).
high
3.45
expanded
0.49
addition,
samples
evaluated
detail
long-term
-80°C
6
months,
short-term
4°C
7
days.
results
showed
that
stable
under
above
conditions.
Sensors,
Год журнала:
2024,
Номер
24(21), С. 6865 - 6865
Опубликована: Окт. 25, 2024
Tylosin
(TYL)
is
a
macrolide
antibiotic
widely
used
in
animal
husbandry.
Due
to
associated
health
risks,
there
demand
for
sensitive
methods
mass
screening
of
TYL
products
origin.
This
article
describes
the
development
lateral
flow
immunoassays
(LFIAs)
detection
using
direct
(anti-TYL
antibodies
conjugated
with
nanoparticles)
and
indirect
antibody
labeling
(anti-species
nanoparticles
combined
native
anti-TYL
antibodies).
The
choice
LFIA
conditions,
such
as
concentrations
hapten-protein
conjugates,
specific
antibodies,
gold
nanoparticle
(GNP)
conjugates
well
incubation
time
reagents
concentration
detergent
sample
buffer,
presented.
achieved
limits
LFIAs
were
0.8
ng/mL
(visual)
0.07
(instrumental),
compared
4
0.4
(instrumental)
case
labeling.
sensitivity
format
was
up
seven
times
higher,
allowing
determination
target
analyte
at
low
concentrations.
ground
meat
ranged
from
76-119%.
Analytical Methods,
Год журнала:
2023,
Номер
15(30), С. 3631 - 3641
Опубликована: Янв. 1, 2023
An
ultrasensitive
LFIA
based
on
multilayered
fluorescent
nanofilm-guided
signal
amplification
was
developed
for
the
rapid
and
quantitative
determination
of
Salmonella
typhimurium
in
complex
food
samples.
Research Square (Research Square),
Год журнала:
2023,
Номер
unknown
Опубликована: Авг. 18, 2023
Abstract
In
this
study,
two
different
concentrations
of
matrix
certified
reference
materials
(CRMs)
were
produced
for
the
accurate
measurement
aflatoxin
M1(AFM1)
in
milk
powder((GBW(E)
100552,
GBW(E)
100553)).
The
raw
material
was
obtained
by
feeding
cows
with
positive
drugs.
homogeneity,
stability
and
characterization
CRMs
examined
liquid
chromatography
tandem
mass
spectrometry
isotope-labeled
internal
standard
method.
value
low
concentration
AFM1
powder
2.45
µg/kg
an
uncertainty
0.43
(coverage
factor
k
=
2,
at
95%
confidence).
high
3.45
expanded
0.49
addition,
samples
evaluated
detail
long-term
-80°C
6
months,
short-term
4°C
7
days.
results
showed
that
stable
under
above
conditions.
