Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 18, 2023
Abstract
In
this
study,
two
different
concentrations
of
matrix
certified
reference
materials
(CRMs)
were
produced
for
the
accurate
measurement
aflatoxin
M1(AFM1)
in
milk
powder((GBW(E)
100552,
GBW(E)
100553)).
The
raw
material
was
obtained
by
feeding
cows
with
positive
drugs.
homogeneity,
stability
and
characterization
CRMs
examined
liquid
chromatography
tandem
mass
spectrometry
isotope-labeled
internal
standard
method.
value
low
concentration
AFM1
powder
2.45
µg/kg
an
uncertainty
0.43
(coverage
factor
k
=
2,
at
95%
confidence).
high
3.45
expanded
0.49
addition,
samples
evaluated
detail
long-term
-80°C
6
months,
short-term
4°C
7
days.
results
showed
that
stable
under
above
conditions.
Microorganisms,
Journal Year:
2024,
Volume and Issue:
12(12), P. 2555 - 2555
Published: Dec. 11, 2024
To
ensure
the
safety
of
foodstuffs,
widespread
non-laboratory
monitoring
for
pathogenic
contaminants
is
in
demand.
A
suitable
technique
this
purpose
lateral
flow
immunoassay
(LFIA)
which
combines
simplicity,
rapidity,
and
productivity
with
specific
immune
detection.
This
study
considered
three
developed
formats
LFIA
Salmonella
Typhimurium,
a
priority
contaminant
milk.
Common
sandwich
all
immunoreagents
pre-applied
to
test
strip
(format
A)
was
compared
incubation
sample
(gold
nanoparticle—antibody)
conjugate,
preceding
processes
B),
sequential
passages
conjugate
along
C).
Under
chosen
conditions,
detection
limits
assay
times
were
3
×
104,
1
105,
105
cells/mL,
10,
15,
20
min
A,
B,
C,
respectively.
The
selected
format
successfully
applied
milk
samples.
sample’s
dilution
fat
content
1.0%
causes
pathogen
detection,
70–110%
revealing
1.5–8.5%
accuracy.
obtained
results
demonstrate
that
allows
lower
concentrations
cells
and,
way,
accelerates
decision-making
food
control.
Analytical Methods,
Journal Year:
2023,
Volume and Issue:
15(30), P. 3631 - 3641
Published: Jan. 1, 2023
An
ultrasensitive
LFIA
based
on
multilayered
fluorescent
nanofilm-guided
signal
amplification
was
developed
for
the
rapid
and
quantitative
determination
of
Salmonella
typhimurium
in
complex
food
samples.
Research Square (Research Square),
Journal Year:
2023,
Volume and Issue:
unknown
Published: Aug. 18, 2023
Abstract
In
this
study,
two
different
concentrations
of
matrix
certified
reference
materials
(CRMs)
were
produced
for
the
accurate
measurement
aflatoxin
M1(AFM1)
in
milk
powder((GBW(E)
100552,
GBW(E)
100553)).
The
raw
material
was
obtained
by
feeding
cows
with
positive
drugs.
homogeneity,
stability
and
characterization
CRMs
examined
liquid
chromatography
tandem
mass
spectrometry
isotope-labeled
internal
standard
method.
value
low
concentration
AFM1
powder
2.45
µg/kg
an
uncertainty
0.43
(coverage
factor
k
=
2,
at
95%
confidence).
high
3.45
expanded
0.49
addition,
samples
evaluated
detail
long-term
-80°C
6
months,
short-term
4°C
7
days.
results
showed
that
stable
under
above
conditions.
