Metabolism of Inulin via Difructose Anhydride I Pathway in Microbacterium flavum
Journal of Agricultural and Food Chemistry,
Год журнала:
2024,
Номер
72(17), С. 9647 - 9655
Опубликована: Апрель 17, 2024
Difructose
anhydride
I
(DFA-I)
can
be
produced
from
inulin,
with
DFA-I-forming
inulin
fructotransferase
(IFTase-I).
However,
the
metabolism
of
through
DFA-I
remains
unclear.
To
clarify
this
pathway,
several
genes
enzymes
related
to
pathway
in
genome
Язык: Английский
Degradation mechanism of difructose dianhydride III in Blautia species
Applied Microbiology and Biotechnology,
Год журнала:
2024,
Номер
108(1)
Опубликована: Ноя. 5, 2024
Abstract
Di-fructofuranose
1,2′:2,3′
dianhydride
(DFA-III)
is
a
cyclic
fructo-disaccharide,
which
produced
by
the
condensation
of
two
fructose
molecules
via
caramelization
or
enzymatic
reaction
inulin
fructotransferase.
A
strain
Blautia
producta
was
known
to
utilize
DFA-III
as
carbohydrate
source;
however,
mechanisms
remain
unclear.
In
this
study,
we
characterized
glycoside
hydrolase
(GH)
family
91
(DFA-IIIase)
from
B.
parvula
NBRC
113351.
Recombinant
Bp
DFA-IIIase
catalyzed
reversible
conversion
inulobiose,
further
degraded
cooperative
action
and
GH32
β-
d
-fructofuranosidase.
utilized
in
several
species
with
gene
cluster
for
degradation
(e.g.,
113351,
hydrogenotrophica
JCM
14656,
wexlerae
35486),
but
not
31267,
does
possess
cluster.
Furthermore,
hansenii
14655,
cannot
metabolize
fructose,
could
DFA-III;
it
degrade
presence
DFA-III-degrading
enzymes.
Fecal
fermentation
tests
showed
that
are
important
gut
microbe
degrading
DFA-III.
Key
points
•
BpDFA-IIIase
first
intestinal
non-pathogenic
bacteria.
widely
conserved
species.
-fructose
through
inulobiose
Язык: Английский
Verification of utilization ability of Bifidobacterium and Lactobacillus to difructose anhydride III in vitro by its hydrolases and their application in Jerusalem artichoke tuber to improve nutrition
International Journal of Biological Macromolecules,
Год журнала:
2024,
Номер
unknown, С. 136634 - 136634
Опубликована: Окт. 1, 2024
Язык: Английский