Metabolism of Inulin via Difructose Anhydride I Pathway in Microbacterium flavum

Journal of Agricultural and Food Chemistry, Journal Year: 2024, Volume and Issue: 72(17), P. 9647 - 9655

Published: April 17, 2024

Difructose anhydride I (DFA-I) can be produced from inulin, with DFA-I-forming inulin fructotransferase (IFTase-I). However, the metabolism of through DFA-I remains unclear. To clarify this pathway, several genes enzymes related to pathway in genome

Language: Английский

Degradation mechanism of difructose dianhydride III in Blautia species

Applied Microbiology and Biotechnology, Journal Year: 2024, Volume and Issue: 108(1)

Published: Nov. 5, 2024

Abstract Di-fructofuranose 1,2′:2,3′ dianhydride (DFA-III) is a cyclic fructo-disaccharide, which produced by the condensation of two fructose molecules via caramelization or enzymatic reaction inulin fructotransferase. A strain Blautia producta was known to utilize DFA-III as carbohydrate source; however, mechanisms remain unclear. In this study, we characterized glycoside hydrolase (GH) family 91 (DFA-IIIase) from B. parvula NBRC 113351. Recombinant Bp DFA-IIIase catalyzed reversible conversion inulobiose, further degraded cooperative action and GH32 β- d -fructofuranosidase. utilized in several species with gene cluster for degradation (e.g., 113351, hydrogenotrophica JCM 14656, wexlerae 35486), but not 31267, does possess cluster. Furthermore, hansenii 14655, cannot metabolize fructose, could DFA-III; it degrade presence DFA-III-degrading enzymes. Fecal fermentation tests showed that are important gut microbe degrading DFA-III. Key points • BpDFA-IIIase first intestinal non-pathogenic bacteria. widely conserved species. -fructose through inulobiose

Language: Английский

Verification of utilization ability of Bifidobacterium and Lactobacillus to difructose anhydride III in vitro by its hydrolases and their application in Jerusalem artichoke tuber to improve nutrition

International Journal of Biological Macromolecules, Journal Year: 2024, Volume and Issue: unknown, P. 136634 - 136634

Published: Oct. 1, 2024

Language: Английский