Transcriptome Analysis Revealed That Cell Wall Regulatory Pathways Are Involved in the Tolerance of Pleurotus ostreatus Mycelia to Different Heat Stresses
Journal of Fungi,
Год журнала:
2025,
Номер
11(4), С. 266 - 266
Опубликована: Март 30, 2025
Pleurotus
ostreatus
is
the
third
largest
cultivated
species
in
China’s
edible
mushroom
industry;
however,
its
agricultural
cultivation
method
easily
affected
by
high-temperature
environments.
To
understand
response
mechanism
of
mycelia
to
heat
stress,
P.
ostreatus,
which
had
been
grown
at
28
°C
for
4
days,
were
subjected
stress
32
and
36
2
followed
RNA-seq
analysis.
These
results
indicate
that,
under
mycelial
growth
was
significantly
inhibited,
cell
membrane
disrupted,
walls
became
thicker,
chitinase
β-1,3-glucanase
activities
decreased.
Transcriptome
analysis
revealed
2118
differentially
expressed
genes
(DEGs)
458
DEGs
identified
stress.
A
total
328
upregulated
or
downregulated
°C.
The
functional
enrichment
these
significant
related
hydrogen
peroxide
metabolism,
oxidoreductase
activity,
ATP
hydrolysis,
wall
structure
composition.
There
a
80
specific
°C,
they
enriched
catalase
wall,
aminoglycan
catabolic
process,
activity.
However,
817
integral
components
membrane,
identification
wall-related
that
hydrophobic
proteins,
Cerato
plateau
laccases,
glycoside
hydrolases
may
respond
qRT-PCR
are
consistent
with
data.
This
study
several
potential
candidate
thermal
response,
laying
foundation
ostreatus.
Язык: Английский
High-Yield-Related Genes Participate in Mushroom Production
Journal of Fungi,
Год журнала:
2024,
Номер
10(11), С. 767 - 767
Опубликована: Ноя. 5, 2024
In
recent
years,
the
increasing
global
demand
for
mushrooms
has
made
enhancement
of
mushroom
yield
a
focal
point
research.
Currently,
primary
methods
developing
high-yield
varieties
include
mutation-
and
hybridization-based
breeding.
However,
due
to
long
breeding
cycles
low
predictability
associated
with
these
approaches,
they
no
longer
meet
demands
high-quality
in
expansive
market.
Modern
molecular
biology
technologies
such
as
RNA
interference
(RNAi)
gene
editing,
including
via
CRISPR-Cas9,
can
be
used
precisely
modify
target
genes,
providing
new
solution
The
genes
divided
into
four
categories
based
on
existing
research
results:
controlling
mycelial
growth
are
very
suitable
genetic
modification;
primordium
formation
directly
or
indirectly
regulated
by
growth;
button
germination
more
difficult
modify;
fruiting
body
development
during
stage.
This
article
reviews
current
status
major
high-yield-related
across
different
stages
stages,
foundation
scientific
basis
using
improve
promote
economic
edible-mushroom
industry.
Язык: Английский