Transcriptome Analysis Revealed That Cell Wall Regulatory Pathways Are Involved in the Tolerance of Pleurotus ostreatus Mycelia to Different Heat Stresses

Journal of Fungi, Journal Year: 2025, Volume and Issue: 11(4), P. 266 - 266

Published: March 30, 2025

Pleurotus ostreatus is the third largest cultivated species in China’s edible mushroom industry; however, its agricultural cultivation method easily affected by high-temperature environments. To understand response mechanism of mycelia to heat stress, P. ostreatus, which had been grown at 28 °C for 4 days, were subjected stress 32 and 36 2 followed RNA-seq analysis. These results indicate that, under mycelial growth was significantly inhibited, cell membrane disrupted, walls became thicker, chitinase β-1,3-glucanase activities decreased. Transcriptome analysis revealed 2118 differentially expressed genes (DEGs) 458 DEGs identified stress. A total 328 upregulated or downregulated °C. The functional enrichment these significant related hydrogen peroxide metabolism, oxidoreductase activity, ATP hydrolysis, wall structure composition. There a 80 specific °C, they enriched catalase wall, aminoglycan catabolic process, activity. However, 817 integral components membrane, identification wall-related that hydrophobic proteins, Cerato plateau laccases, glycoside hydrolases may respond qRT-PCR are consistent with data. This study several potential candidate thermal response, laying foundation ostreatus.

Language: Английский

High-Yield-Related Genes Participate in Mushroom Production

Journal of Fungi, Journal Year: 2024, Volume and Issue: 10(11), P. 767 - 767

Published: Nov. 5, 2024

In recent years, the increasing global demand for mushrooms has made enhancement of mushroom yield a focal point research. Currently, primary methods developing high-yield varieties include mutation- and hybridization-based breeding. However, due to long breeding cycles low predictability associated with these approaches, they no longer meet demands high-quality in expansive market. Modern molecular biology technologies such as RNA interference (RNAi) gene editing, including via CRISPR-Cas9, can be used precisely modify target genes, providing new solution The genes divided into four categories based on existing research results: controlling mycelial growth are very suitable genetic modification; primordium formation directly or indirectly regulated by growth; button germination more difficult modify; fruiting body development during stage. This article reviews current status major high-yield-related across different stages stages, foundation scientific basis using improve promote economic edible-mushroom industry.

Language: Английский