Kinetic Analysis and Starch Digestion Product Composition Reveal the Subtle Relationship between the Anthocyanidin Structure and Inhibitory Activity on Pancreatic α-Amylase

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Фев. 10, 2025

The polyphenols anthocyanins and their aglycones, anthocyanidins, can control postprandial blood glucose through inhibition of α-amylase. In this study, 10 were purified from fruit materials 6 anthocyanidins obtained by acid hydrolysis. identities these compounds confirmed LC-ESI-MS/MS. Inhibition activities on α-amylase higher than those anthocyanins. kinetic assay using 2-chloro-4-nitrophenyl α-maltotrioside detection properties in the composition starch digestion products revealed that an increased number hydroxyl groups (−OH) B-ring promotes overall ability compound enzyme enhances competitive ability. It also increases activity formation enzymatic with degrees polymerization 2–3. mechanism appears to be interaction −OHs key amino residues around active site, as molecular docking. Methylation at 3′-OH stabilize hydrogen bonds between residues. However, simultaneous methylation 3′ 5′-OHs caused a decrease transformed mixed mode into pure inhibition, which was attributed steric hindrance.

Язык: Английский

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Super Stable Coating Based on Ovalbumin and Tannic Acid for Hydrophilic and Antibacterial Functionalization of Polymer Materials

ACS Applied Materials & Interfaces, Год журнала: 2025, Номер unknown

Опубликована: Фев. 11, 2025

Surface modification of polymer materials is crucial in the biomedical field, as it can endow with new properties, including high efficacy and durability a low risk infection. Here, we propose simple, green, reliable surface strategy using ovalbumin (OVA) tannic acid (TA). The hydrogen bonds hydrophobic interactions revealed between OVA TA molecules make OVA/TA composite tenacious stable. subsequent coatings adhered firmly on five two-step impregnation method were highly hydrophilic repellent to bacterial adhesion. Taking advantage reducing ability TA, silver ions reduced situ form OVA/TA-AgNPs coatings, which could inhibit broad spectrum bacteria, especially some drug-resistant strains. In addition, both exhibit good biocompatibility. This reliable, stable, biobased coating holds great promise for enhancing versatility biomaterial modification.

Язык: Английский

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Potential Antidiabetic Activity of Nordihydroguaiaretic Acid: An Insight into Its Inhibitory Mechanisms on Carbohydrate-Hydrolyzing Enzymes, the Binding Behaviors with Enzymes, and In Vivo Antihyperglycemic Effect

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Март 28, 2025

The inhibitory mechanisms and binding behaviors of nordihydroguaiaretic acid (NDGA) to α-glucosidase/α-amylase were investigated by in vitro multispectroscopic methods silico modeling technique. results demonstrated that NDGA reversibly uncompetitively inhibited α-glucosidase, exhibiting stronger inhibition than acarbose, while it displayed noncompetitive against α-amylase. Additionally, could spontaneously bind mainly through hydrogen bonds hydrophobic forces, thus altering the spatial structure enzymes reducing their catalytic activity. presence crowding reagents/polysaccharides/undigested milk proteins would decrease ability NDGA, whereas fatty acids exhibited opposite phenomenon on α-glucosidase. Furthermore, antidiabetic activity vivo was evaluated using diabetic Drosophila model induced a high-sugar diet. It found significantly reduced glucose levels Drosophila. These findings suggested potential inhibitor be used as nutritional adjuvant prevent diabetes.

Язык: Английский

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Exploring the Utility of Prunus mahaleb Extracts as a Source of Natural Bioactive Compounds for Functional Applications

Food Science & Nutrition, Год журнала: 2025, Номер 13(4)

Опубликована: Март 28, 2025

ABSTRACT Prunus mahaleb has garnered attention as a potent medicinal agent and functional component. We aimed to detect the chemical composition biological activities of several parts (fruit, leaves, twigs) P. . Biological were assessed for antioxidant properties, enzyme inhibition, mutagenic/antimutagenic effects, antibacterial efficacy. Antioxidant capabilities evaluated using various assays, including DPPH, ABTS, CUPRAC, FRAP, phosphomolybdenum, metal chelating. The constituents extracts identified quantified HPLC‐ESI‐MS/MS method. effects inhibition examined on some enzymes, AChE, BChE, tyrosinase, amylase, glucosidase. Ames test was used evaluate mutagenic antimutagenic properties plant extracts. Furthermore, broth microdilution assay employed possible against microorganisms. methanol extract twigs showed superior (DPPH: 388.39 mg TE/g; ABTS: 701.50 CUPRAC: 459.05 FRAP: 264.99 TE/g). demonstrated highest tyrosinase inhibitory activity (61.91 KAE/g). A total 40 metabolites, mainly flavonoids, detected through analysis, revealing that ferulic acid, naringenin, herniarin predominant compounds. In test, tested exhibited no potential. antimutagenicity ethyl acetate from leaves particularly efficient frameshift base pair substitution mutations induced by recognized mutagens. metabolic activation system amplified these strong rates ranging 85% 98%. results indicated effectiveness at dosages between 6.25 0.195 mg/mL, effective Sarcina lutea, Bacillus cereus , Candida albicans Staphylococcus aureus Our findings indicate can serve versatile raw material development health‐promoting applications, medicines, cosmeceuticals, nutraceuticals.

Язык: Английский

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Applying Isothermal Titration Calorimetry and Saturation Transfer Difference-NMR to Study the Mode of Interaction of Flavan-3-ols with α-Amylase to Understand Their Impact on Starch Hydrolysis

Journal of Agricultural and Food Chemistry, Год журнала: 2025, Номер unknown

Опубликована: Апрель 4, 2025

For flavan-3-ols, significant effects to prevent the development of diabetes mellitus are postulated. Inter alia, this is attributed inhibitory on intestinal α-amylase, in particular for high-molecular-weight procyanidins. In order gain a deeper insight into mode interaction and resulting α-amylase inhibition, between monomers (+)-catechin (CAT) (-)-epicatechin (EC), dimers procyanidin (PC) B1 PC B2, trimer C1 their inhibition porcine pancreatic were investigated. Weak interactions determined by isothermal titration calorimetry (ITC), with no clear difference even observable C1. Data from saturation transfer (STD)-NMR experiments supported these results respect reversible interactions. The detailed NMR signal assignments revealed that formation rotamers solvent-dependent, which might explain differences strength both diastereomers. contrast accumulating an increasing degree polymerization when monitoring hydrolysis natural substrate starch novel continuous approach ITC. By combining data studies, we propose protein aggregation occurs presence flavan-3-ol oligomers, responsible effects. This rather irreversible not susceptible detection ITC STD-NMR was also CD spectroscopy.

Язык: Английский

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Novel Natural Candidates for Replacing Synthetic Additives in Nutraceutical and Pharmaceutical Areas: Two Senna Species (S. alata (L.) Roxb. and S. occidentalis (L.) Link)

Food Science & Nutrition, Год журнала: 2024, Номер 13(1)

Опубликована: Дек. 22, 2024

ABSTRACT Senna alata (L.) Roxb. and occidentalis Link (family Fabaceae) are commonly used in different systems of traditional medicine to treat ailments. The present study was designed determine the phytoconstituents, antioxidant, enzyme inhibition, antimicrobial activities methanolic extract from leaves these two species. A total 75 phenolic compounds belonging dihydroxybenzoic acids, dihydroxycinnamic flavonoid C‐glycosides, O‐glycosides, aglycones, anthraquinone glycosides, aglycones were identified. Flavonoid C‐glycosides only found S. while sennosides A, B, C detected . In line with its higher flavonoids contents, exerted significantly ( p < 0.05) antiradical (2,2‐diphenyl‐1‐picrylhydrazy (DPPH) = 58.36 mg trolox equivalent (TE)/g; 2,2′‐azino‐bis(3‐ethylbenzothiazoline‐6‐sulfonic acid (ABTS) 118.86 TE/g), ions reducing (cupric antioxidant capacity (CUPRAC) 93.85 TE/g; ferric power (FRAP) 50.42 (1.39 mmol TE/g) than revealed inhibitory effect against butyrylcholinesterase (1.67 galantamine (GALAE)/g), tyrosinase (45.07 KAE/g) 45.07 kojic (KAE)/g), α‐glucosidase (0.73 acarbose (ACAE)/g), α‐amylase (2.95 ACAE/g) enzymes. Both species showed high antibacterial antifungal remarkable activity by Trichoderma viride (minimum inhibition concentration (MIC) 1 mg/mL), similar that Ketoconazole. utilized molecular docking, mechanics Poisson–Boltzmann surface area (MM/PBSA) free energy calculations, dynamics simulations evaluate binding interactions between glycosides various bacterial enzymes, including targets Escherichia coli Staphylococcus aureus findings suggest like sennoside chrysophanol exhibit strong affinities, stable interactions, potential as inhibitors, especially vital proteins such MurE 30S ribosome S3. conclusion, our underscore biopharmaceutical species, suggesting their significance sources bioactive agents for health‐related applications.

Язык: Английский

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Insights into one drug, multi-target aspects of polyphenols for diabetes management: in vitro, in vivo, and clinical evidence

Phytochemistry Reviews, Год журнала: 2024, Номер unknown

Опубликована: Дек. 7, 2024

Язык: Английский

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Inhibitory mechanism of α-glucosidase by liquiritigenin and its combined effect with acarbose: Multi-spectroscopic analyses and molecular docking simulation

Food Bioscience, Год журнала: 2024, Номер 63, С. 105659 - 105659

Опубликована: Дек. 9, 2024

Язык: Английский

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