Food and Chemical Toxicology, Год журнала: 2022, Номер 162, С. 112899 - 112899
Опубликована: Фев. 26, 2022
Язык: Английский
Journal of Cellular and Molecular Medicine, Год журнала: 2021, Номер 25(12), С. 5358 - 5371
Опубликована: Май 10, 2021
As a common air pollutant, formaldehyde is widely present in nature, industrial production and consumer products. Endogenous mainly produced through the oxidative deamination of methylamine catalysed by semicarbazide-sensitive amine oxidase (SSAO) ubiquitous human body fluids, tissues cells. Vascular endothelial cells smooth muscle are rich this formaldehyde-producing enzyme easily damaged owing to consequent cytotoxicity. Consistent with this, increasing evidence suggests that cardiovascular system stages heart development also susceptible harmful effects formaldehyde. Exposure from different sources can induce disease such as arrhythmia, myocardial infarction (MI), failure (HF) atherosclerosis (AS). In particular, long-term exposure high concentrations pregnant women more likely affect embryonic cause malformations than low Specifically, ability mouse embryos effect clearance far lower rat embryos, readily allowing its accumulation. Formaldehyde may exert toxic on inducing stress cardiomyocyte apoptosis. This review focuses current progress understanding influence underlying mechanisms development.
Язык: Английский
Процитировано
83
Journal of Nanobiotechnology, Год журнала: 2021, Номер 19(1)
Опубликована: Окт. 30, 2021
Abstract Titanium (Ti) implants are widely used in dentistry and orthopedics owing to their excellent corrosion resistance, biocompatibility, mechanical properties, which have gained increasing attention from the viewpoints of fundamental research practical applications. Also, numerous studies been carried out fine-tune micro/nanostructures Ti and/or incorporate chemical elements improve overall implant performance. Zinc oxide nanoparticles (nano-ZnO) well-known for good antibacterial properties low cytotoxicity along with ability synergize a variety substances, received increasingly widespread as biomodification materials implants. In this review, we summarize recent progress on nano-ZnO modified Ti-implants. Their preparation methods Ti-implants introduced, followed by further presentation antibacterial, osteogenic, anti-corrosion these Finally, challenges future opportunities proposed. Graphical
Язык: Английский
Процитировано
79
Free Radical Biology and Medicine, Год журнала: 2023, Номер 201, С. 76 - 88
Опубликована: Март 16, 2023
Язык: Английский
Процитировано
40
Acta Biomaterialia, Год журнала: 2024, Номер unknown
Опубликована: Сен. 1, 2024
Язык: Английский
Процитировано
14
Molecular Therapy — Nucleic Acids, Год журнала: 2021, Номер 26, С. 295 - 306
Опубликована: Июль 29, 2021
Accumulating evidence shows that tRNA-derived fragments are a novel class of functional small non-coding RNA; however, their roles in aortic dissection (AD) still unknown. In this study, we found 5′-tiRNA-Cys-GCA was significantly downregulated human and mouse models dissection. The abnormal proliferation, migration, phenotypic transition vascular smooth muscle cells (VSMCs) played crucial role the initiation progression dissection, with as potential switching regulator, because its overexpression inhibited proliferation migration VSMCs increased expression contractile markers. addition, verified signal transducer activator transcription 4 (STAT4) direct downstream target 5′-tiRNA-Cys-GCA. We STAT4 upregulation oxidized low-density lipoprotein (ox-LDL)-treated VSMCs, which promoted cell transformation, reversed by Furthermore, treatment reduced incidence prevented malignant process angiotensin II- β-aminopropionitrile-induced AD mice. conclusion, our findings reveal is regulator pathological via signaling pathway, providing clinical for development future strategies IntroductionThe definition rupture intima due to various reasons, causing blood from orifice enter wall, forming arterial wall separation..1Sun Y. Xiao Sun H. Zhao Z. Zhu J. Zhang L. Dong Han T. Jing Q. Zhou miR-27a regulates remodeling targeting endothelial cells' apoptosis interaction dissection.Theranostics. 2019; 9: 7961-7975Crossref PubMed Scopus (21) Google Scholar,2Cheng M. Yang Xin Li Zong He X. Yu Non-coding RNAs dissection: From biomarkers therapeutic targets.J. Cell. Mol. Med. 2020; 24: 11622-11637Crossref (20) Scholar Eighty percent patients die rupture;3Thompson R.W. Detection management aneurysms.N. Engl. 2002; 346: 1484-1486Crossref (45) hence, once occurs, patient should be immediately hospitalized given surgical intervention. (VSMCs), an important constituent vessels, believed play onset AD.4Liu S. Jiang Tang N. Tian Ponnusamy Tariq M.A. Lian Understanding RNA (ncRNA) stent restenosis.Atherosclerosis. 2018; 272: 153-161Abstract Full Text PDF (36) have prominent feature called flexibility, enables them alternate between (differentiated) synthetic (dedifferentiated) phenotypes.5Li F.J. C.L. Luo X.J. Peng T.L. Involvement MiR-181b-5p/HMGB1 Pathway Ang II-induced Phenotypic Transformation Smooth Muscle Cells Hypertension.Aging Dis. 10: 231-248Crossref (24) Scholar, 6Wang C.Q. G.Y. Huang H.Y. Y.S. Ji Z.C. Shen Z.Y. MicroRNA-134-5p Regulates Media Degeneration through Inhibiting VSMC Switch Migration Thoracic Aortic Dissection.Mol. Ther. Nucleic Acids. 16: 284-294Abstract (28) 7Davis-Dusenbery B.N. Wu C. Hata A. Micromanaging differentiation modulation.Arterioscler. Thromb. Vasc. Biol. 2011; 31: 2370-2377Crossref (180) 8Fu P. Wang Zou et al.Nicotine: Regulatory mechanisms atherosclerosis progression.Food Chem. Toxicol. 2021; 151: 112154Crossref (18) Contractile characterized high α-smooth actin (α-SMA) capabilities. contrast, contain low levels markers exhibit migration.9Owens G.K. Kumar M.S. Wamhoff B.R. Molecular regulation disease.Physiol. Rev. 2004; 84: 767-801Crossref (2536) accelerate occurrence alternations caused stimulants, such (ox-LDL) platelet-derived growth factor BB (PDGF-BB).10Inamoto Kwartler C.S. Lafont A.L. Liang Y.Y. Fadulu V.T. Duraisamy Willing Estrera Safi Hannibal M.C. al.TGFBR2 mutations alter phenotype predispose thoracic aneurysms dissections.Cardiovasc. Res. 2010; 88: 520-529Crossref (100) 11Jones J.A. Beck Barbour J.R. Zavadzkas Mukherjee R. Spinale F.G. Ikonomidis J.S. Alterations cellular constituents during aneurysm development: myofibroblast-mediated remodeling.Am. Pathol. 2009; 175: 1746-1756Abstract (54) 12Wang Liu Yuan Suppression miR-4463 promotes treated Ox-LDL.Cell Tissue 383: 1155-1165Crossref (7) Vascular involves subsequently damaging state balance.13McMurtry Bonnet Dyck Haromy Hashimoto K. Michelakis E.D. Dichloroacetate prevents reverses pulmonary hypertension inducing artery apoptosis.Circ. 95: 830-840Crossref (371) Scholar,14Yang Guo Meng Aung L.H.H. Targeting epigenome in-stent restenosis: therapy.Mol. 23: 1136-1160Abstract addition also link progression.15Liao W.L. Tan M.W. Xu Brahma-related gene 1 inhibits directly up-regulating Ras-related associated diabetes pathophysiologic processes dissection.J. Thorac. Cardiovasc. Surg. 2015; 150 (1292–301.e2)Abstract (16) Scholar,16Wang Fu W. D. Association phenotypes extracellular matrix disorders dissection.J Vasc 2012; 56: 1698-1709Abstract (72) Recent studies confirmed compared control group, samples more common.17Xiao Ma G. al.MicroRNA-22 Inhibits Apoptosis Cell p38MAPKα Remodeling 22: 1051-1062Abstract (13) Scholar,18Shi B. MST1 down-regulation decreasing apoptosis.Eur. Pharmacol. Sci. 2044-2051PubMed Therefore, elucidation involved conversion under conditions essential new diagnosis AD.The stress-induced (tiRNAs) kind newly discovered produced mature tRNAs.14Yang Scholar,19Li Sheng tRNA-Derived Small RNA: A Novel Non-Coding RNA.Genes (Basel). 246Crossref (148) 20Li J.X. Piwi-interacting (piRNAs) targets cardiovascular diseases.Angiogenesis. 19-34Crossref (26) 21Yang P.F. miRNAs diagnostic disease particular focus on WO2010091204.Expert Opin. Pat. 2017; 27: 1021-1029Crossref (32) Under specific conditions, tRNAs cleaved angiogenin (ANG), position anticodon loop will produce two kinds tiRNAs. These tiRNAs 5′-tiRNAs 3′-tiRNAs, length approximately 28–36 nts.16Wang Scholar,22Saikia Krokowski Guan B.J. Ivanov Parisien Hu G.F. Anderson Pan Hatzoglou Genome-wide identification quantitative analysis tRNA induced stress.J. 287: 42708-42725Abstract (147) 23Yamasaki Angiogenin cleaves translational repression.J. 185: 35-42Crossref (572) 24He Expression profiles transfer RNA-derived atherosclerosis.J. 25: 7052-7065Crossref (9) 25Zhang R.C. function molecular mechanism formaldehyde heart development.J. 5358-5371Crossref (15) 26Yang Zhan Shan Ding al.Blood TfR+ exosomes separated pH-responsive method deliver chemotherapeutics tumor therapy.Theranostics. 7680-7696Crossref (50) report tiRNAs, may biological functions, abnormally expressed or participate certain diseases, cancer, neurological, metabolic, viral infectious diseases.26Yang 27Shen Yan Transfer halves: biogenesis, functions diseases.J. (Berl.). 96: 1167-1176Crossref (104) 28Zhang Shi Cao Gao Ren Ning al.Identification characterization ancient enriched serum associating active infection.J. 2014; 6: 172-174Crossref 29Qi Selective arginine deprivation single injection non-uptake deiminase nanocapsules sustained inhibition.Nanoscale. 12: 24030-24043Crossref 30Qi Xue Stability Maintenance Protein Drugs Organic Coatings Based Nanogels.Pharmaceutics. 115Crossref (11) Notably, recent 5′-tiRNA regulating cancer cells31Mo Mao Wei F. fragment, 5′-tiRNAVal, suppresses Wnt/β-catenin pathway FZD3 breast cancer.Cancer Lett. 457: 60-73Crossref (66) biomarker ischemia-reperfusion damage death.32Elkordy Rashad Shehabeldeen Mishima E. Niizuma Abe Tominaga assessment vitro model rat neuronal PC12 cells.Brain 1714: 8-17Crossref However, relationship diseases whether altering unknown.Signal member STAT family.33Verhoeven Tilborghs Jacobs De Waele Quatannens Deben Prenen Pauwels Trinh X.B. Wouters al.The controversy family members cancer.Semin. Cancer 60: 41-56Crossref (113) protein located surfaces.34Lv Ye deficiency protects against neointima formation following injury mice.J. Cardiol. 74: After phosphorylation JAK proteins, proteins nucleus, combine transcripts, regulate genes related differentiation.35Guo Zarella Wagner W.D. atherosclerosis.Exp. 2006; 81: 15-22Crossref highly organs, including testis, spleen, heart, brain, thymus.36Yamamoto Kobayashi Arai Miura O. Hirosawa Miyasaka cDNA cloning, chromosome mapping gene: both STAT1 mapped 2q32.2-->q32.3.Cytogenet. Genet. 1997; 77: 207-210Crossref several can detected main apoptosis.34Lv Scholar,35Guo hypothesized cells.Our study explores AD. Our shed light identify regulatory pathways VSMCs. Future research focused present interventional AD.ResultsDifferential localization vivo vitroWe first level aorta. normal aorta, co-localized α-SMA, suggesting most likely (Figure 1A). Moreover, tissue lower than aorta (Figures 1A 1B). Consistent these data, mice 2.5× 1C). suggest serve protective To further investigate function, mimicked treating ox-LDL (100 μg/mL), plays vital VSMCs.37Xue Cho J.Y. Functional ginsenosides Panax ginseng Ginseng 45: 22-31Crossref (46) 38Tang Noncoding mellitus.Cardiovasc. 36: e12436Crossref 39Bai Chu X.M. NLRP3 inflammasome dysfunction.Cell Death 11: 776Crossref (116) 40He Long PEBP1P2 Suppresses Proliferative Switching Proliferation Atherosclerosis.Mol. 84-98Abstract (34) 41Wang noncoding XXYLT1-AS2 adhesion binding FUS HUVEC.Atherosclerosis. 298: 58-69Abstract 42Liu al.Insights into circRNA angiogenesis implications.Atherosclerosis. 14-26Abstract (55) 43Yu Jie Kim kinase inhibitor BX795 inflammatory response multiple kinases.Biochem. 174: 113797Crossref (29) Interestingly, after treatment, exhibited downward trend time-dependent manner 1D). Together, results indicate abundantly close association AD; were interested studying AD.Overexpression then synthesized transfected mimics 24 h transfection 2A), indicating successfully incorporated cells. counting kit-8 (CCK-8) experiment performed same showed 2B). 5-ethynyl-2′-deoxyuridine (EdU) assay inhibitory effect 2C). suppressed ability wound healing 2D) Transwell assays 2E). next determined observed 2F), specifically while calponin (CNN1) myosin heavy chain (SMHC) did not show significant changes.9Owens By rates change 2H), detecting apoptosis-related p53 caspase-3 (c-caspase-3; Figure 2G).44Du Qian Chen Zheng Pu Xia Aloin Preconditioning Attenuates Hepatic Ischemia/Reperfusion Injury TLR4/MyD88/NF-κB Signal Vivo Vitro.Oxid. Longev. 2019: 3765898Crossref (30) Scholar,45Su Lv X.W. Y.L. Cai R.P. Dai R.X. X.H. W.K. Kong B.H. Exosomal LINC00174 derived attenuates myocardial I/R p53-mediated autophagy apoptosis.Mol. 1304-1322Abstract summary, mainly regulated affect VSMCs.Figure 2Overexpression vitroShow full caption(A) used overexpress using real-time PCR. (B C) (B) cell-light EdU staining (C) measure at 0, 12, 24, 36 h. (D E) analyzed wound-healing (D) (E) assay. (F) Western blotting analyses conducted (G H) TUNEL (G) western blot (H) detect Data presented mean ± SD. Each repeated least three times. Scale bars, 200 μm. ∗p < 0.05; ∗∗p 0.01; ∗∗∗p 0.001; ∗∗∗∗p 0.0001; ns, significant.View Large Image ViewerDownload Hi-res image Download (PPT)Knockdown vitroThe knockdown 3A), consequently 3B 3C). Simultaneously, accelerated 3D 3E), 3F). any 3G 3H). validating 3Knockdown inhibitors down-express qPCR. CCK-8 down-expression ware (PPT)5′-tiRNA-Cys-GCA binds downregulates STAT4Investigation 5′-tiRNA-Cys-GCA-mediated KEGG ontology (GO) identified total 153 predicted 4A). revealed only 9 migration. targets, namely STAT4, PC nanoparticles (PCNP), cysteine rich secretory LCCL domain containing 2 (CRISPLD2), pulled down 4B). PCR (qPCR) remaining six (JAK3, KCNE3, HBEGF, TLR4, TREM1, TNFAIP8L2) undetermined Ct values, very levels. had highest enrichment, implying it probable Using miRanda TargetScan software, site 4C) structure score, free energy, Context+ values 145, −24.79, −0.303, respectively. Further displayed degree conservation humans, mice, rats 4C). Additional experiments mRNA 4D) 4E), correspondingly expression. Finally, dual luciferase reporter fluorescence intensities overexpressed wild-type plasmid reduced, there no mutant group. Taken together, bind activities.Figure 45′-tiRNA-Cys-GCA STAT4Show Predicted kyoto encyclopaedia genomes (KEGG) enrichment predictions, literature review pull-down selected. Pull-down Theoretically STAT4. qPCR NC STAT4-3′ UTR-WT mut co-transfected NC. Luciferase activity decreased (PPT)STAT4 ox-LDL-induced transformation 5′-tiRNA-Cys-GCAWe designed interfering (siRNA)-mediated validate 5′-tiRNA-Cys-GCA/STAT4 axis First, siRNA knocks
Язык: Английский
Процитировано
42
ACS Applied Materials & Interfaces, Год журнала: 2022, Номер 14(1), С. 2058 - 2070
Опубликована: Янв. 3, 2022
Oral gene therapy has emerged as a potential optimal treatment for ulcerative colitis (UC). Nucleic acid drugs possessing versatility can not only inhibit inflammation but realize colon mucosal healing, fulfilling the clinical objective of UC therapy. However, effective accumulation and distribution oral nucleic in remain considerable challenge. Furthermore, current delivery systems pay more attention to colon, while which plays key role treatment, never catches researchers. Here, we used miR-320 model drug develop kind multistage-responsive nanocomplexes (MSNs) based on polymeric nanocapsules alginate. MSNs possess pH responsiveness stomach, enzyme colonic lumen, redox cytoplasm. In vivo imaging results showed that reach within 2 h effectively release lumen. The further deliver submucosal layer even muscular layer. Moreover, decreased activity myeloperoxidase proinflammatory cytokines exhibited anti-inflammatory by inhibiting phosphorylation IκBα AKT, reducing enhancing repair. Therefore, successfully alleviate improving promoting translational application UC.
Язык: Английский
Процитировано
38
Cardiovascular Research, Год журнала: 2022, Номер 119(3), С. 729 - 742
Опубликована: Авг. 24, 2022
Abstract Aims Nicotine, a major component of tobacco, is an important factor contributing to atherosclerosis. However, the molecular mechanisms underlying link between nicotine and atherosclerosis are unclear. As extracellular vesicles (EVs) involved in intercellular communication atherosclerosis, we investigated whether their influence on arterial pathophysiology under stimulation. Methods results EVs from serum smokers (smoker-EVs) were significantly increased exacerbated endothelial inflammation, as well apoptosis according functional studies. Meanwhile, inhibition blunted nicotine-induced progression, injection promoted progression ApoE–/– mice. Furthermore, quantitative reverse transcription-polymerase chain reaction analysis revealed remarkable increase miR-155 levels smoker-EVs, which was correlated with carotid plaque formation patients measured by ultrasound imaging. Moreover, CD14 (representing derived monocytes), indicating that monocytes source smoker-EVs. DNA methylation transcription HIF1α may contribute monocytes, assessed bisulfite conversion sequencing chromatin immunoprecipitation. Mechanistically, encapsulated induced cell dysfunction directedly targeting BCL2, MCL1, TIMP3, BCL6, activating NF-κB pathway, verified series biological experiments. Injecting nicotine-stimulated triggered vascular injury mice, whereas weakened this effect. Conclusion Our findings EV-dependent mechanism nicotine-aggravated Accordingly, propose EV-based intervention strategy for management.
Язык: Английский
Процитировано
36
Journal of Ginseng Research, Год журнала: 2022, Номер 47(2), С. 237 - 245
Опубликована: Авг. 6, 2022
Ginsenoside Rg2 (Rg2) has a variety of pharmacological activities and provides benefits during inflammation, cancer, other diseases. However, there are no reports about the relationship between atherosclerosis.We used 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) to detect cell viability in vascular smooth muscle cells (VSMCs) human umbilical vein endothelial (HUVECs). The expression inflammatory factors HUVECs phenotypic transformation-related marker VSMCs were detected at mRNA levels. Western blot method was inflammation pathways transformation protein rat carotid balloon injury model performed explore effect on vivo.Rg2 decreased induced by lipopolysaccharide HUVECs-without affecting viability. These events depend blocking regulation NF-κB p-ERK signaling pathway. In VSMCs, can inhibit proliferation, migration, platelet derived growth factor-BB (PDGF-BB)-which may contribute its anti-atherosclerotic role. rats with injury, reduce intimal proliferation after regulate pathway response, also suppress VSMCs.These results suggest that exert cellular level animal level, which more sufficient basis for ginseng as functional dietary regulator.
Язык: Английский
Процитировано
31
Pharmacological Research, Год журнала: 2023, Номер 196, С. 106932 - 106932
Опубликована: Сен. 20, 2023
Aortic dissection (AD) presents a medical challenge for clinicians. Here, to determine the role of novel small non-coding piRNA-823 (piR-823) in AD, murine and human aorta from patients with AD were used. A high expression levels piR-823 found AD. Using performed loss- gain-of-function assays vitro vivo, we explore regulatory effect on vascular smooth muscle cells (VSMCs) obviously facilitates proliferation, migration, phenotypic transformation VSMCs or without nicotine treatment. directly binds suppresses histone deacetylase 1 (HDAC1) expression, regulates acetylation 3 (H3) via H3K9ac H3K27ac, eventually, VSMC functions To consolidate our findings, model was performed, observed that antagomir strongly inhibited pathogenesis through regulating remodeling. Thus, study finds potential target prevention treatment strategy nicotine-induced
Язык: Английский
Процитировано
20
Pflügers Archiv - European Journal of Physiology, Год журнала: 2024, Номер 476(6), С. 875 - 888
Опубликована: Фев. 20, 2024
Abstract Electronic cigarettes (e-cigarettes), as alternative nicotine delivery methods, has rapidly increased among youth and adults in recent years. However, cardiovascular safety is an important consideration regarding e-cigarettes usage. e-cigarette emissions, including nicotine, propylene glycol, flavorings, nitrosamine, metals, might have adverse effects on health. A large body of epidemiological evidence indicated that are considered independent risk factor for rates disease occurrence death. The incidence mortality various types disease, such cardiac arrhythmia, hypertension, acute coronary syndromes, heart failure, a modest growth vapers (users e-cigarettes). Although the underlying biological mechanisms not been fully understood, studies validated oxidative stress, inflammation, endothelial dysfunction, atherosclerosis, hemodynamic effects, platelet function play roles which work human body. This minireview consolidates discusses links between disease.
Язык: Английский
Процитировано
9