Frontiers in Immunology,
Год журнала:
2024,
Номер
15
Опубликована: Ноя. 29, 2024
Lung
metastasis
has
garnered
significant
attention
due
to
its
prevalent
occurrence.
Pre-metastatic
niche
(PMN)
establishment
is
a
critical
prerequisite
for
the
onset
of
lung
metastasis.
Emerging
evidence
indicates
that
long
noncoding
RNAs
(lncRNAs)
play
pivotal
roles
in
metastatic
cascade
lungs.
However,
relationship
between
lncRNA
expression
profiles
and
formation
PMN
remains
uncharacterized.
This
study
aims
explore
potential
lncRNAs
context
pre-metastatic
microenvironment.
Journal of Advanced Research,
Год журнала:
2024,
Номер
unknown
Опубликована: Апрель 1, 2024
Necroptosis
triggered
by
H2O2
is
hypothesized
to
be
a
critical
factor
in
the
rupture
of
atherosclerotic
plaques,
which
may
precipitate
acute
cardiovascular
events.
Nevertheless,
specific
regulatory
molecules
this
development
remain
unclear.
We
aims
elucidate
mechanism
from
perspective
circular
RNA.
There
are
few
studies
on
circRNA
VSMCs
necroptosis.
The
objective
our
research
shed
light
intricate
roles
that
circHIPK3
plays
process
necroptosis
and
plaques
prone
rupture.
Our
study
elucidates
molecular
mechanisms
regulates
vulnerable
plaque
formation
through
targeted
proteins.
Identifying
at
cellular
level
offers
framework
for
understanding
progression
stability
regulation,
as
well
potential
biomarker
prognosis
susceptible
plaques.
collected
clinical
vascular
tissue
HE
staining
Masson
determine
presence
Then,
NCBI
database
was
used
screen
out
with
elevated
expression
tissue,
up-regulated
circRNA,
circHIPK3,
verified
qRT-PCR
FISH.
Further,
we
synthesized
circHIPK3′s
small
interference
sequence
overexpressed
plasmid
vitro,
its
regulation
effect
under
physiological
pathological
conditions
WB,
PI
staining.
Through
RNA
pull
down,
mass
spectrometry
immunoprecipitation,
DRP1
identified
binding
protein
positively
regulated
circHIPK3.
Meanwhile,
basis
silencing
DRP1,
mediated
DRP1.
Finally,
validated
ApoE-/-
mice.
investigated
highly
expressed
increase
promoted
induced
VSMCs.
CircHIPK3
leading
an
elevation
mitochondrial
division
rate,
resulting
increased
reactive
oxygen
species
impaired
function,
ultimately
formation.
interact
involve
Mitochondrial
damage
VSMCs,
Silencing
vivo
can
reduce
findings
have
applications
providing
diagnostic
biomarkers
Advanced Healthcare Materials,
Год журнала:
2024,
Номер
unknown
Опубликована: Авг. 1, 2024
Abstract
Aortic
dissection
(AD)
is
a
severe
cardiovascular
disease
necessitating
active
therapeutic
strategies
for
early
intervention
and
prevention.
Nucleic
acid
drugs,
known
their
potent
molecule‐targeting
properties,
offer
potential
genetic
suppression
of
AD.
Piwi‐interacting
RNAs,
class
small
hold
promise
managing
diseases.
Limited
research
on
these
RNAs
AD
exists.
This
study
demonstrates
that
an
antagomir
targeting
heart‐apoptosis‐associated
piRNA
(HAAPIR)
effectively
regulates
vascular
remodeling,
mitigating
occurrence
progression
through
the
myocyte
enhancer
factor
2D
(Mef2D)
matrix
metallopeptidase
9
(MMP9)
pathways.
Green
tea‐derived
plant
exosome‐like
nanovesicles
(PELNs)
are
used
oral
administration
antagomir.
The
antagomir‐HAAPIR‐nanovesicle
complex,
after
purification
optimization,
exhibits
high
packing
rate,
while
resistant
to
enzyme
digestion.
Administered
mice,
complex
targets
aortic
lesion,
reducing
incidence
improving
survival.
Moreover,
MMP9
Mef2D
expression
decrease
significantly,
inhibiting
phenotypic
conversion
human
smooth
muscle
cells.
PELNs
encapsulate
antagomir‐HAAPIR
maintaining
stability,
mediating
transport
into
bloodstream,
delivering
sites.
Thus,
HAAPIR
target
persistent
clinical
prevention
treatment,
nanovesicle‐encapsulated
nucleic
acids
promising
providing
insights
other
targets.
iScience,
Год журнала:
2024,
Номер
27(4), С. 109502 - 109502
Опубликована: Март 16, 2024
Constitutive
explorations
indicate
a
correlation
between
circular
RNAs
(circRNAs)
and
cardiovascular
diseases.
However,
the
involvement
of
circRNAs
in
endothelial
recuperation
in-stent
restenosis
(ISR)
remains
underexplored.
CircTMEM165
has
first
been
reported
to
be
highly
expressed
hypoxic
human
umbilical
vein
cells
(HUVECs).
Here,
we
identified
that
circTMEM165
was
downregulated
ISR
patients,
inversely
correlating
with
severity.
Functionally,
found
abundant
cells,
inhibiting
inflammation,
adhesion.
Particularly,
observed
could
alleviate
HUVECs
apoptosis
mitochondrial
fission
induced
by
lipopolysaccharide
(LPS).
Mechanistically,
circTMEM165,
as
miR-192-3p
sponge,
enhancing
SCP2
expression,
which
serves
critical
regulator
biological
functions.
Moreover,
Immunology,
Год журнала:
2024,
Номер
173(1), С. 53 - 75
Опубликована: Июнь 12, 2024
The
cross-regulation
of
immunity
and
metabolism
is
currently
a
research
hotspot
in
life
sciences
immunology.
Metabolic
immunology
plays
an
important
role
cutting-edge
fields
such
as
metabolic
regulatory
mechanisms
immune
cell
development
function,
targets
immune-related
disease
pathways.
Protein
post-translational
modification
(PTM)
key
epigenetic
mechanism
that
regulates
various
biological
processes
highlights
metabolite
functions.
Currently,
more
than
400
PTM
types
have
been
identified
to
affect
the
functions
several
proteins.
Among
these,
PTMs,
particularly
newly
histone
or
non-histone
acylation
modifications,
can
effectively
regulate
functions,
diseases
system,
well
diseases.
Thus,
drugs
aimed
at
targeted
substantial
therapeutic
potential
regulating
immunity,
indicating
new
direction
for
further
clinical
translational
research.
This
review
summarises
characteristics
seven
novel
lysine
including
succinylation,
S-palmitoylation,
lactylation,
crotonylation,
2-hydroxyisobutyrylation,
β-hydroxybutyrylation
malonylation,
their
association
with
thereby
providing
valuable
references
diagnosis
treatment
disorders
associated
modifications.
Clinical and Translational Medicine,
Год журнала:
2024,
Номер
14(8)
Опубликована: Июль 31, 2024
Abstract
Background
The
relationship
between
noncoding
RNAs
(ncRNAs)
and
human
diseases
has
been
a
hot
topic
of
research,
but
the
study
ncRNAs
in
cardiovascular
(CVDs)
is
still
its
infancy.
PIWI‐interacting
RNA
(piRNA),
small
ncRNA
that
binds
to
PIWI
protein
maintain
genome
stability
by
silencing
transposons,
was
widely
studied
germ
lines
stem
cells.
In
recent
years,
piRNA
shown
be
involved
key
events
multiple
CVDs
through
various
epigenetic
modifications,
revealing
potential
value
as
new
biomarker
or
therapeutic
target.
Conclusion
This
review
explores
origin,
degradation,
function,
mechanism
important
role
CVDs,
promising
targets
were
summarized.
provide
strategy
for
treatment
lay
theoretical
foundation
future
research.
Key
points
can
used
target
biomaker
CVDs.
influences
apoptosis,
inflammation
angiogenesis
regulating
modificaions.
Critical
knowledge
gaps
remain
unifying
nomenclature
PIWI‐independent
function.
Transfer
RNA-derived
small
RNAs,
a
recently
identified
class
of
noncoding
play
crucial
role
in
regulating
gene
expression
and
are
implicated
cerebrovascular
diseases.
However,
the
specific
biological
roles
mechanisms
transfer
RNAs
intracranial
aneurysms
(IAs)
remain
unclear.
In
this
study,
we
that
RNA-Asp-GTC
derived
fragment
(tRF-AspGTC)
is
highly
expressed
IA
tissues
both
humans
mice.
tRF-AspGTC
promotes
formation
by
facilitating
phenotypic
switching
vascular
smooth
muscle
cells,
increasing
matrix
metalloproteinase
9
expression,
inducing
oxidative
stress
inflammatory
responses.
Mechanistically,
binds
to
galectin-3,
inhibiting
tripartite
motif
29-mediated
ubiquitination
stabilizing
galectin-3.
This
stabilization
activates
toll-like
receptor
4/MyD88/nuclear
factor
kappa
B
pathway,
further
driving
inflammation.
Clinically,
circulating
exosomal
demonstrates
strong
diagnostic
efficacy
for
IAs
as
an
independent
risk
occurrence.
These
findings
highlight
potential
promising
biomarker
therapeutic
target
IAs.
