Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(12), С. 14270 - 14290
Опубликована: Дек. 18, 2024
Dendrobium
devonianum
is
an
important
medicinal
plant,
rich
in
flavonoid,
with
various
pharmacological
activities
such
as
stomachic
and
antioxidant
properties.
In
this
study,
we
integrated
metabolome
transcriptome
analyses
to
reveal
metabolite
gene
expression
profiles
of
D.
devonianum,
both
green
(GDd)
purple-red
(RDd)
semi-annual
annual
stems.
A
total
244
flavonoid
metabolites,
mainly
flavones
flavonols,
were
identified
annotated.
Cyanidin
delphinidin
the
major
anthocyanidins,
cyanidin-3-O-(6″-O-p-Coumaroyl)
glucoside
delphinidin-3-O-(6″-O-p-coumaroyl)
being
highest
relative
content
RDd.
Differential
metabolites
significantly
enriched,
biosynthesis,
anthocyanin
flavone
flavonol
biosynthesis
pathways.
Transcriptomic
analysis
revealed
that
high
levels
structural
genes
for
main
reasons
color
changes
Based
on
correlation
weighted
co-expression
network
(WGCNA)
analysis,
CHS2
(chalcone
synthase)
UGT77B2
(anthocyanidin
3-O-glucosyltransferase)
candidate
involved
stem
pigmentation.
addition,
key
transcription
factors
(TFs),
including
three
bHLH
(bHLH3,
bHLH4,
bHLH5)
two
MYB
(MYB1,
MYB2),
which
may
be
regulation
identified.
This
study
provides
new
perspectives
efficacy
components
flavonoids
regulation.
Foods,
Год журнала:
2024,
Номер
13(18), С. 2959 - 2959
Опубликована: Сен. 18, 2024
The
main
and
most
commonly
known
biological
function
of
nucleobases,
nucleosides,
nucleotides
is
usually
associated
with
the
fact
that
they
are
building
blocks
nucleic
acids.
However,
these
compounds
also
belong
to
plant
secondary
metabolites,
although
in
role
have
attracted
less
attention
than
others,
e.g.,
terpenes,
phenolics,
or
alkaloids.
former
important
constituents
human
diet,
as
ingredients
tea
herbs,
endowing
them
specific
taste
qualities
pharmacological
activities.
Liquid
chromatography–mass
spectrometry
seems
be
analytical
method
permits
identification
determination
nucleotides,
along
other
metabolites.
goal
this
review
discuss
detail
aspects
mass
spectrometric
detection
selected
herbs.
An
conclusion
interest
should
performed
not
only
on
basis
[M
+
H]+/[M
−
H]−
ions
but
confirmed
by
respective
product
ions;
however,
discussed
review,
it
may
sometimes
problematic.
It
clear
all
difficulties
encountered
when
analyzing
material
caused
complexity
analyzed
samples
need
analyze
different
classes
compounds,
absolutely
does
debase
any
mentioned
papers.
Current Issues in Molecular Biology,
Год журнала:
2024,
Номер
46(12), С. 14270 - 14290
Опубликована: Дек. 18, 2024
Dendrobium
devonianum
is
an
important
medicinal
plant,
rich
in
flavonoid,
with
various
pharmacological
activities
such
as
stomachic
and
antioxidant
properties.
In
this
study,
we
integrated
metabolome
transcriptome
analyses
to
reveal
metabolite
gene
expression
profiles
of
D.
devonianum,
both
green
(GDd)
purple-red
(RDd)
semi-annual
annual
stems.
A
total
244
flavonoid
metabolites,
mainly
flavones
flavonols,
were
identified
annotated.
Cyanidin
delphinidin
the
major
anthocyanidins,
cyanidin-3-O-(6″-O-p-Coumaroyl)
glucoside
delphinidin-3-O-(6″-O-p-coumaroyl)
being
highest
relative
content
RDd.
Differential
metabolites
significantly
enriched,
biosynthesis,
anthocyanin
flavone
flavonol
biosynthesis
pathways.
Transcriptomic
analysis
revealed
that
high
levels
structural
genes
for
main
reasons
color
changes
Based
on
correlation
weighted
co-expression
network
(WGCNA)
analysis,
CHS2
(chalcone
synthase)
UGT77B2
(anthocyanidin
3-O-glucosyltransferase)
candidate
involved
stem
pigmentation.
addition,
key
transcription
factors
(TFs),
including
three
bHLH
(bHLH3,
bHLH4,
bHLH5)
two
MYB
(MYB1,
MYB2),
which
may
be
regulation
identified.
This
study
provides
new
perspectives
efficacy
components
flavonoids
regulation.
