Relationship between Protein Digestibility and the Proteolysis of Legume Proteins during Seed Germination

Molecules, Год журнала: 2023, Номер 28(7), С. 3204 - 3204

Опубликована: Апрель 4, 2023

Legume seed protein is an important source of nutrition, but generally it less digestible than animal protein. Poor digestibility in legume seeds and seedlings may partly reflect defenses against herbivores. Protein changes during germination typically increase proteolysis digestibility, by lowering the levels anti-nutrient protease inhibitors, activating proteases, breaking down storage proteins (including allergens). Germinating sprouts also show striking increases free amino acids (especially asparagine), their roles host defense or other processes are not known. While net effect to proteins, extent improvement species- strain-dependent. Further research needed highlight which contribute most improved sprouted seeds. Such knowledge could guide selection varieties that more development food preparations digestible, potentially combining with factors altering such as heating fermentation. Techniques characterize shifts make-up, activity degradation need draw on traditional analytical approaches, complemented proteomic peptidomic analysis mass spectrometry-identified peptide breakdown products.

Язык: Английский

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Mass Spectrometry Characterization of the SDS-PAGE Protein Profile of Legumins and Vicilins from Chickpea Seed

Foods, Год журнала: 2024, Номер 13(6), С. 887 - 887

Опубликована: Март 14, 2024

Chickpea (

Язык: Английский

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Fluidized bed drying of chickpeas: Developing a new drying schedule to reduce protein denaturation and remove trypsin inhibitors

Journal of Food Engineering, Год журнала: 2023, Номер 351, С. 111515 - 111515

Опубликована: Март 23, 2023

Язык: Английский

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Comprehensive Review of Plant Protein Digestibility: Challenges, Assessment Methods, and Improvement Strategies

Applied Sciences, Год журнала: 2025, Номер 15(7), С. 3538 - 3538

Опубликована: Март 24, 2025

Plant-based proteins are increasingly recognized for their environmental, ethical, and nutritional benefits. However, digestibility varies due to factors such as molecular structure, amino acid composition, processing methods. This review comprehensively analyzes methods used assess plant protein digestibility, including in vivo, vitro, ex vivo approaches. While studies, particularly those using pigs, considered the gold standard, vitro models offer cost-effective reproducible alternatives simulating digestion. Additionally, antinutritional present can hinder necessitating strategies fermentation, enzymatic hydrolysis, high-pressure treatments enhance bioavailability. Advances evaluation techniques, Digestible Indispensable Amino Acid Score (DIAAS) dynamic digestion models, more precise assessments of quality. By systematically comparing these methods, this aims guide food scientists manufacturers selecting appropriate improve quality plant-based products. Understanding mechanisms influencing is essential optimizing formulations supporting broader adoption sustainable sources human diets.

Язык: Английский

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Allergen Stability in Food Allergy: A Clinician’s Perspective

Current Allergy and Asthma Reports, Год журнала: 2023, Номер 23(10), С. 601 - 612

Опубликована: Сен. 4, 2023

Abstract Purpose of Review The globally rising food allergy prevalence is associated with the urgent need for new disease prevention methods, efficient treatment, and reliable risk assessment methods characterization allergens. Due to inter-individual variations in digestive system, allergens are degraded a different extent each person. Food processing also influences allergen digestion. Recent Findings In this review, we provide an overview system focus on relevance allergy. Main proteins causing allergic reactions evaluated, combined role digestion stability highlighted. Finally, clinical implications knowledge discussed. Summary literature shows that digestibility dependent processing, conditions, matrix. Digestion affects allergenicity. It currently not possible predict immunogenicity solely based protein stability.

Язык: Английский

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Effect of Germination on the Digestion of Legume Proteins

Foods, Год журнала: 2024, Номер 13(17), С. 2655 - 2655

Опубликована: Авг. 23, 2024

As one of the main sources plant protein, it is important to improve protein digestibility legumes. Faced with population growth and increasing environmental pressures, essential find a green approach. Germination meets this requirement, in process natural growth, some enzymes are activated make dynamic changes itself; at same time, other substances (especially anti-nutrient factors) can also be degraded by or their properties (water solubility, etc.), thereby reducing binding finally improving beans under combined influence these factors The whole low-carbon, environmentally friendly safe. Therefore, paper summarizes provide reference for subsequent development soybean functional food, especially germination food.

Язык: Английский

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In vitro digestion of milk proteins including intestinal brush border membrane peptidases. Transepithelial transport of resistant casein domains

Food Research International, Год журнала: 2022, Номер 157, С. 111238 - 111238

Опубликована: Апрель 9, 2022

The use of enzymes from the brush border membrane (BBM) in simulated gastrointestinal digestion milk proteins has been evaluated. With this purpose, resistant sequences casein and whey after INFOGEST vitro with without BBM have analyzed by tandem mass spectrometry. revealed additional cleavages to those found pancreatic enzymes, although number total identified peptides decreased due reduction peptide size. These new were mainly attributed activity amino- carboxy-peptidases, which was also reflected higher concentration free amino acids digests BBM. peptidome compared that previously obtained aspirated human jejunum oral administration same substrates. addition did not change significantly profile, it allowed identification digests. However, none models able reproduce large variety vivo. In addition, transepithelial transport six β-casein derived digestion, including opioid β-casomorphin-7, results point importance nature N- C-terminal end for rate through Caco-2 cell monolayer. Therefore, as a supplementary step can be considered bioavailability studies since final sequence determine absorption peptides.

Язык: Английский

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Allergenic potential of novel plant-derived ingredients

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 430 - 440

Опубликована: Янв. 1, 2024

Язык: Английский

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Bioactive peptides released from legumes during gastrointestinal digestion

Elsevier eBooks, Год журнала: 2024, Номер unknown, С. 261 - 303

Опубликована: Янв. 1, 2024

Язык: Английский

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Impact of porcine brush border membrane enzymes on INFOGEST in vitro digestion model: A step forward to mimic the small intestinal phase

Food Research International, Год журнала: 2024, Номер 197, С. 115300 - 115300

Опубликована: Ноя. 5, 2024

Brush border membrane (BBM) enzymes greatly affect the bioaccessibility and bioavailability of food nutrients. Despite their physiological importance, a step simulating final stage intestinal digestion has not yet been included in harmonized protocols for vitro digestion, primarily due to challenges replicating dynamics degradation. Herein, we propose an advancement toward more physiologically relevant method, complementing static gastric-duodenal INFOGEST model with missing small phase. BBM hydrolase activity, incubation time, at pH 7.2 were established reproduce conditions. Skim milk powder, as protein food, was subjected digestion. Immediately after duodenal phase, digesta supplemented vesicles purified from pig jejunum. To comply dynamic nature balance spontaneous inactivation hydrolases, supplements added every two hours throughout 6 h time. Peptide degradation monitored each by amino acid analysis, free α-amino group assay, HPLC, LC-MS/MS. Hydrolysis peptidases led significant increase acids, reflecting known level adsorption (>90 %) humans eating proteins. LC-MS/MS analysis demonstrated that hydrolases erode progressively peptides released gastro-duodenal processing up stable sequence motifs. The approach described is particularly when endpoint identifying peptide sequences cannot be further hydrolysed digestive or determine bio-accessibility.

Язык: Английский

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